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Flow cytometry data acquisition system

A data acquisition system and flow cytometer technology, which is applied to instruments, scientific instruments, measuring devices, etc., can solve problems such as low recovery accuracy, parameter extraction errors, and cannot be missing information recovery, and achieve high flexibility and fast operation speed. Effect

Pending Publication Date: 2019-04-05
GUANGXI NORMAL UNIV
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Problems solved by technology

However, the rectangular compensation method cannot completely restore the missing information, the restoration accuracy is low, and it is easy to cause errors in parameter extraction, resulting in an increase in the coefficient of variation of the flow cytometer

Method used

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Embodiment Construction

[0030] The specific implementation manner of the present invention will be described in more detail below with reference to schematic diagrams. Advantages and features of the present invention will be apparent from the following description and claims. It should be noted that all the drawings are in a very simplified form and use imprecise scales, and are only used to facilitate and clearly assist the purpose of illustrating the embodiments of the present invention.

[0031] Such as figure 1 As shown, the present invention provides a flow cytometer data acquisition system, comprising a sequentially connected data acquisition module 1, FPGA module 2 and MCU module 3, the FPGA module 2 controls the data acquisition module 1 to collect 8 channels Cell pulse electrical signals, and orderly read 8 channels of cell pulse electrical signals from the data acquisition module 1 to identify the peak value, width and area of ​​each of the cell pulse electrical signals, when the MCU modul...

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Abstract

The invention provides a flow cytometry data acquisition system. The flow cytometry data acquisition system comprises a data acquisition module, an FPGA module and an MCU module which are connected insequence, wherein the FPGA module controls the data acquisition module to acquire 8 cell pulse electrical signals, and reads the 8 cell pulse electrical signals in an ordered mode from the data acquisition module to identify the peak value, width and area of each of the cell pulse electrical signals. When the MCU module receives a control signal sent by the FPGA module, a recognition result is read from the FPGA module and sent to a host computer. The flow cytometry data acquisition system effectively combines an FPGA with an STM32, fully exerts the processing advantages of the FPGA for high-speed parallel data, and can well realize the high-speed parallel acquisition and simultaneous remote transmission storage of the cell pulse electrical signals, so that a high operation speed is ensured and high flexibility is achieved.

Description

technical field [0001] The invention relates to the technical field of flow cytometer digital signal processing, in particular to a flow cytometer data acquisition system. Background technique [0002] Flow cytometer is a new high-tech instrument integrating laser technology, fluid mechanics technology, photoelectric measurement technology, electronic computer technology, cell fluorescence chemistry technology and cell immunity technology. It also has the function of analyzing and sorting cells. Collect and measure the scattered light and labeled fluorescence generated by single cells or other particles in high-speed, straight-line flow in the suspension when they pass through the detection area, so as to realize high-speed one-by-one multi-parameter quantitative analysis. It has a wide range of applications in the fields of cell biology, cell dynamics, immunology, hematology and oncology. [0003] The flow cytometer completes the reception of the forward scattered light, s...

Claims

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Application Information

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IPC IPC(8): G01N15/14
CPCG01N15/1456
Inventor 宋树祥于丽华
Owner GUANGXI NORMAL UNIV
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