A method for cultivating Cordyceps militaris by inoculating 5th instar larvae of castor silkworm

A technology of castor silkworm and Cordyceps militaris, which is applied in the field of Cordyceps cultivation, can solve the problem of less Cordyceps militaris, and achieve the effects of preventing strain degradation, low contamination rate of miscellaneous bacteria, and rich content of effective active ingredients

Active Publication Date: 2021-07-13
湖南省蚕桑科学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Castor silkworm Cordyceps militaris is a kind of Cordyceps militaris, and there are few reports on successfully artificially cultivated Cordyceps militaris related to castor silkworm

Method used

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  • A method for cultivating Cordyceps militaris by inoculating 5th instar larvae of castor silkworm
  • A method for cultivating Cordyceps militaris by inoculating 5th instar larvae of castor silkworm
  • A method for cultivating Cordyceps militaris by inoculating 5th instar larvae of castor silkworm

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Effect test

Embodiment 1

[0033] This embodiment includes the following steps:

[0034] (1) Host selection of Cordyceps militaris: Select healthy silkworm bodies of the fifth instar 3d castor silkworm. After the 5th instar is fed, the castor leaves are continuously fed at a temperature of 25°C and a relative humidity of 60%, ensuring ventilation and timely detoxification. Sand, select robust castor silkworm larvae for subsequent use;

[0035] (2) Expanded cultivation of strain liquid preparation solution: select a first-generation hybrid variety with strong disease resistance, silkworm larvae with strong infectivity, stable herb production, high-quality and high-yield fruiting bodies of Cordyceps militaris slanted mother species, and 1 piece of soybean-sized pupa Cordyceps slanted mother species were inoculated on 1L of ready-made solid plate medium added with 5wt% silkworm chrysalis powder. After 10 days of activation, under aseptic operation, the strains were evenly divided into soybean-sized strains...

Embodiment 2

[0045] This embodiment includes the following steps:

[0046] (1) Host selection of Cordyceps militaris: Select the healthy castor-oil silkworm body of the fifth instar 4d. After the 5th instar is fed, the temperature is 28°C and the relative humidity is 65%, and the castor leaves are continuously fed to ensure ventilation and timely removal. Sand, select robust castor silkworm larvae for subsequent use;

[0047] (2) Expanded cultivation of strain liquid preparation solution: select a first-generation hybrid variety with strong disease resistance, silkworm larvae with strong infectivity, stable herb production, high-quality and high-yield fruiting bodies of Cordyceps militaris slanted mother species, and 1 piece of soybean-sized pupa Cordyceps slanted mother species were inoculated on 1L of ready-made solid plate medium added with 7wt% silkworm chrysalis powder. After 9 days of activation, under aseptic operation, the strains were evenly divided into soybean-sized strains, and...

Embodiment 3

[0057] This embodiment includes the following steps:

[0058] (1) Selection of the host of Cordyceps militaris; select healthy silkworm bodies of the fifth instar 5d castor silkworm. After the 5th instar is fed, the castor leaves are continuously fed at a temperature of 30°C and a relative humidity of 70% to ensure ventilation and timely sand removal. , select robust castor silkworm larvae for subsequent use;

[0059] (2) Expand the cultivation of the liquid preparation solution of the strain, select a first-generation hybrid variety with strong disease resistance, silkworm larvae with strong infectivity, stable weeding, high-quality and high-yield fruiting bodies of Cordyceps militaris slant female species, and 1 piece of soybean-sized pupa Cordyceps slanted mother species were inoculated on 1L of ready-made solid plate medium added with 8wt% silkworm chrysalis powder. After 7 days of activation, under aseptic operation, the strains were evenly divided into soybean-sized stra...

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Abstract

A method of using Cordyceps militaris to inoculate 5th instar larvae of ricinus militaris to cultivate Cordyceps militaris, comprising the following steps: (1) selecting a host of Cordyceps militaris; (2) expanding the culture of strain liquid preparation solution; (3) host ricinus fragilis Post-inoculation treatment; (4) Cordyceps militaris fruit body cultivation management: after inoculating the slanted mother species of Cordyceps militaris on the solid plate medium for activation, inoculating in the liquid medium, inoculating in the healthy castor silkworm larvae after expanding the culture and infecting rigidity , put the infected silkworm body that has not yet germinated in a sterile transparent plastic box for cultivation and management, that is, it is obtained. The method makes full use of the advantages of the larvae of the castor silkworm, such as large individual, soft body, rich nutrition, etc. to cultivate the fruiting body; , The content of effective active ingredients is rich, suitable for large-scale production, and the development prospect is broad.

Description

technical field [0001] The invention relates to a method for cultivating Cordyceps silkworm by using Cordyceps militaris to inoculate 5th instar larvae of castor silkworms, and belongs to the technical field of Cordyceps cultivation. Background technique [0002] Cordyceps is a kind of medicinal and edible fungus with remarkable nourishing effect, and has the reputation of "living gold". As the old saying goes: "It is better to have a handful of Cordyceps than a cart full of gold and jade". Cordyceps sinensis, Cordyceps militaris and Cicada are all the best of Chinese medicinal Cordyceps. Cordyceps militaris (Cordyceps militaris), also known as Cordyceps militaris, is a model species of the genus Cordyceps in the family Ergotaceae. A complex of fungi and insect shells that coexist. The medicinal value of Cordyceps militaris has been recorded in many medical bibliographies. Its medicinal properties are mild, and it has a wide range of medicinal and edible properties withou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/50A01G18/20A01G18/60A01G18/69A01G18/30
CPCA01G18/20A01G18/30A01G18/50A01G18/60A01G18/69
Inventor 张俊李一平颜新培廖模祥钱荷英张月华李刚张晓米雅兰徐瑛雷语
Owner 湖南省蚕桑科学研究所
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