A method for cultivating Cordyceps militaris by inoculating 5th instar larvae of castor silkworm
A technology of castor silkworm and Cordyceps militaris, which is applied in the field of Cordyceps cultivation, can solve the problem of less Cordyceps militaris, and achieve the effects of preventing strain degradation, low contamination rate of miscellaneous bacteria, and rich content of effective active ingredients
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Embodiment 1
[0033] This embodiment includes the following steps:
[0034] (1) Host selection of Cordyceps militaris: Select healthy silkworm bodies of the fifth instar 3d castor silkworm. After the 5th instar is fed, the castor leaves are continuously fed at a temperature of 25°C and a relative humidity of 60%, ensuring ventilation and timely detoxification. Sand, select robust castor silkworm larvae for subsequent use;
[0035] (2) Expanded cultivation of strain liquid preparation solution: select a first-generation hybrid variety with strong disease resistance, silkworm larvae with strong infectivity, stable herb production, high-quality and high-yield fruiting bodies of Cordyceps militaris slanted mother species, and 1 piece of soybean-sized pupa Cordyceps slanted mother species were inoculated on 1L of ready-made solid plate medium added with 5wt% silkworm chrysalis powder. After 10 days of activation, under aseptic operation, the strains were evenly divided into soybean-sized strains...
Embodiment 2
[0045] This embodiment includes the following steps:
[0046] (1) Host selection of Cordyceps militaris: Select the healthy castor-oil silkworm body of the fifth instar 4d. After the 5th instar is fed, the temperature is 28°C and the relative humidity is 65%, and the castor leaves are continuously fed to ensure ventilation and timely removal. Sand, select robust castor silkworm larvae for subsequent use;
[0047] (2) Expanded cultivation of strain liquid preparation solution: select a first-generation hybrid variety with strong disease resistance, silkworm larvae with strong infectivity, stable herb production, high-quality and high-yield fruiting bodies of Cordyceps militaris slanted mother species, and 1 piece of soybean-sized pupa Cordyceps slanted mother species were inoculated on 1L of ready-made solid plate medium added with 7wt% silkworm chrysalis powder. After 9 days of activation, under aseptic operation, the strains were evenly divided into soybean-sized strains, and...
Embodiment 3
[0057] This embodiment includes the following steps:
[0058] (1) Selection of the host of Cordyceps militaris; select healthy silkworm bodies of the fifth instar 5d castor silkworm. After the 5th instar is fed, the castor leaves are continuously fed at a temperature of 30°C and a relative humidity of 70% to ensure ventilation and timely sand removal. , select robust castor silkworm larvae for subsequent use;
[0059] (2) Expand the cultivation of the liquid preparation solution of the strain, select a first-generation hybrid variety with strong disease resistance, silkworm larvae with strong infectivity, stable weeding, high-quality and high-yield fruiting bodies of Cordyceps militaris slant female species, and 1 piece of soybean-sized pupa Cordyceps slanted mother species were inoculated on 1L of ready-made solid plate medium added with 8wt% silkworm chrysalis powder. After 7 days of activation, under aseptic operation, the strains were evenly divided into soybean-sized stra...
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