A liquid chromatography detection device for detection of monosaccharide components in tea and its use method

Abstract

The invention discloses a liquid chromatographic detection device for detecting monosaccharide components in tea and a method for using the same, which belong to the field of tea detection. The inside of the tube is filled with filler, and the outer end of the column tube near the outer nut is provided with an external thread, and the end of the outer nut close to the column tube is dug with an internal thread groove matching the external thread, and the outer nut is far away from the column tube. There is a liquid inlet at one end, and the liquid inlet is connected with the internal thread groove. A sealing ring and a main sieve plate are respectively arranged between the column tube and the outer nut. The sealing ring is sleeved on the outside of the column tube, which can realize the A liquid cavity is set inside the ring to self-fill the cracked sealing ring to prolong the service life of the sealing ring. By setting an air cavity inside the sealing ring, the user is reminded that the sealing ring is severely cracked and needs to be replaced, thereby reducing the probability of leakage. , to reduce the detection error.

Description

technical field [0001] The invention relates to the field of tea detection, and more specifically relates to a liquid chromatography detection device for detection of monosaccharide components in tea and a method for using the same. Background technique [0002] Sugars are a large class of organic compounds with broad-spectrum chemical structures and physiological functions that exist in nature. They are mainly formed by photosynthesis of green plants. They are not only energy sources (energy storage materials such as glycogen) and fuels for animals and plants, but also Intermediate products of metabolic processes play a very important role in biological processes and are another important class of biomacromolecules besides proteins and nucleic acids. The carbohydrates in tea account for 20%-25% of the dry matter. In addition to the cellulose, lignin and pectin that make up the cell wall, there are also some free sugars, combined glycolipids and protein sugars, which are the...

AI Technical Summary

Problems solved by technology

[0004] When using liquid chromatography to detect tea leaves, a chromatographic column is generally required. In order to ensure the tightness of the connection of the chromatographic column, a layer of sealing ring is generally installed between the nut and the column tube to prevent the liquid from leaking out of the column tube. However, after long-term use, the sealing ring is prone to aging and cracking. Generally, the aging and cracking sealing ring cannot be used continuously, resulting in waste of materials and increasing the detection cost. If the cracked sealing ring is not replaced in time, it will lead to liquid chromatography. Leakage occurs during the detection process, which in turn affects the progress of the entire detection process, resulting in errors in the detection results

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