A kind of mesoporous silica nanoparticles loaded with norcantharidinic acid and preparation method thereof
A technology of mesoporous and mesoporous silicon dioxide of cantharidinic acid is applied in the directions of pharmaceutical formulations, active ingredients of heterocyclic compounds, and medical preparations of non-active ingredients, etc., and can solve the problems such as the inability of norcantharidin to maintain the drug concentration, and the like, To achieve the effect of improving bioavailability, improving synthetic yield and prolonging half-life in vivo
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0023] The preparation method of mesoporous silica nanoparticles containing norcantharidic acid includes:
[0024] (1)NH 2 -Preparation of MSNs: 0.5g CTAB is dissolved in 40g ultrapure water, and 2mol / L NaOH solution is added to adjust the pH to 11; after magnetic stirring at 80℃ for 1h, the mixture containing 1.5mL TEOS and 0.5mL APTES is added dropwise Add to CTAB aqueous solution, react for 2h, stand for 4h, centrifuge at 15000r / min for 45min, wash with acidic ethanol to remove the template CTAB, the volume ratio of absolute ethanol to hydrochloric acid in the acidic ethanol is 10:1; wash with water, centrifuge, and freeze Dry to get NH 2 -MSNs.
[0025] (2)DM-NCTD@NH 2 -Preparation of MSN: Weigh NH 2 -MSNs freeze-dried powder 50mg, add to 10mL of 1mg / mL DM-NCTD aqueous solution, stir for 24h; 15000r / min high-speed centrifugation for 60min, remove free DM-NCTD, wash with water, centrifuge and freeze-dry to obtain DM-NCTD @NH 2 -MSN.
[0026] Observe the prepared NH with a high-p...
Embodiment 2
[0042] H22 in vitro cytotoxicity test:
[0043] Take H22 in the logarithmic growth phase, collect the suspension cell liquid, adjust the cell concentration, and seed it in a 96-well plate with a seeding density of 1×10 4 / Well; after the 96-well plate is incubated in a constant temperature incubator for 24 hours, the original drug (DM-NCTD), DM-NCTD@NH 2 -MSN group was added at different concentrations (0.8, 4, 20, 100, 500μg / mL), and 3 multiple wells were set for each concentration to calculate the average value; sterilized PBS was added to the outermost wells of the 96-well plate Buffer 0.2mL to resist the edge effect; 96-well plates were incubated for 24 and 48 hours respectively. Take the 96-well plates according to the time point, add 20μL / well of 5mg / ml MTT solution, and incubate at 37°C for 4 hours; In addition to the liquid in the well, add DMSO, 100μL / well; detect the absorbance at 490nm by the microplate reader; calculate and count the tumor inhibition rate, among which,...
PUM
Property | Measurement | Unit |
---|---|---|
particle diameter | aaaaa | aaaaa |
concentration | aaaaa | aaaaa |
encapsulation rate | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com