Bacillus amyloliquefaciens 327 and application thereof

A technology of amylolytic spores and bacillus, which is applied in the direction of application, bacteria, and fungicides, and can solve problems such as increased dosage of pesticides in the field, impact on farmers' income, and losses

Pending Publication Date: 2019-06-07
GUIZHOU TOBACCO CORP QIANXINAN CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In addition, capsicum anthracnose is one of the main diseases in capsicum production, which can cause damage throughout the growth period of capsicum, causing great losses
Guizhou Province is one of the main cultivation areas of pepper in my country. The long-term continuous cropping of pepper has caused the continuous expansion of the incidence of ant

Method used

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  • Bacillus amyloliquefaciens 327 and application thereof
  • Bacillus amyloliquefaciens 327 and application thereof
  • Bacillus amyloliquefaciens 327 and application thereof

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Embodiment Construction

[0016] Below in conjunction with accompanying drawing and specific embodiment the invention is further introduced:

[0017] 1. Isolation and screening of Bacillus amyloliquefaciens 327

[0018] 1. Materials and methods

[0019] 1.1 Materials

[0020] Soil samples were collected from the plots with severe tobacco black shank disease in Anlong County, Qianxinan Prefecture, Guizhou Province from 2017 to 2018. The 5-point sampling method was used to combine soil samples from the same plot to calculate 1 A soil sample. Use paper bags to collect soil samples, and disinfect soil collection tools in advance to avoid mixing soil microorganisms.

[0021] 1.2 Medium

[0022] LB medium is used for the isolation and storage of Bacillus and plate confrontation experiments; PDA medium is mainly used for plate confrontation experiments; protease detection medium, cellulase detection medium, siderophilic detection medium, phosphatase detection medium The medium is used to detect whether B...

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Abstract

The invention discloses bacillus amyloliquefaciens 327. The bacillus amyloliquefaciens 327 is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M2019045. The invention also discloses an application of the bacillus amyloliquefaciens 327 to prevention and treatment of tobacco black shank. The bacillus amyloliquefaciens 327 disclosed by the inventionhas obvious capacity for antagonizing the tobacco black shank bacteria, and the capacity for antagonizing pepper anthracnose.

Description

technical field [0001] The invention relates to a bacillus amyloliquefaciens 327 and an application thereof, belonging to the technical field of biological control. Background technique [0002] Tobacco black shank (Phytophthoranicotianae) is one of the most destructive soil-borne diseases in tobacco production in Guizhou. The economic loss caused by this disease is nearly ten million yuan every year, seriously affecting the economic income of tobacco farmers. At present, chemical control is mostly used in production, but the main chemical has a long service life, the resistance of pathogenic bacteria is increasing year by year, and the negative effects of pesticide residues and environmental pollution are also gradually increasing. Therefore, the use of biological control to control tobacco diseases is a hot research field at present. [0003] Bacillus (Bacillus spp.) is currently the most commonly used microbial species in biological control research. Bacillus has the pro...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P1/00A01P3/00C12R1/07
Inventor 张恒朱迪夏木李金星马骏彭丽娟丁海霞何现梅康若谷
Owner GUIZHOU TOBACCO CORP QIANXINAN CORP
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