Esophageal squamous carcinoma diagnosis and treatment target and application
A technology for esophageal squamous cell carcinoma and reagents, applied in the field of biomedicine, can solve the problems of lack of quantitative standards, cannot exclude the influence of subjective factors, etc., to achieve the effect of improving treatment effect, avoiding insufficient treatment intensity, and meeting detection requirements
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0047] Example 1 Screening for gene markers associated with esophageal squamous cell carcinoma
[0048] 1. Sample collection
[0049] 57 cases of esophageal squamous cell carcinoma tissues and adjacent tissues were collected, including 17 patients with histological grade I (G1), 21 patients with histological grade II (G2), and histological grade III (G3) 19 patients. Three samples were taken from each group for detection and analysis of gene expression profiles, screening of differentially expressed genes, and verification experiments were performed in all samples of each group.
[0050] 2. Preparation of RNA samples
[0051] Extraction of tissue total RNA using TRIZOL method
[0052] 1) Mince the tissue with scissors, add 1ml Trizol, shake on the shaker for 1min; place at room temperature for 10min to completely decompose the ribosomes.
[0053] 2) Add 200 μl of chloroform (chloroform), close the cap tightly, shake vigorously for 15 seconds, and let stand at room temperat...
Embodiment 2
[0069] Example 2 QPCR sequencing to verify the differential expression of the LINC01322 gene
[0070] 1. Large-scale QPCR verification of the differential expression of the LINC01322 gene.
[0071] 2. The RNA extraction steps are as described in Example 1.
[0072] 3. Reverse transcription:
[0073] The reverse transcription kit (Takara code: DRR047A) of TAKARA Company was used for operation.
[0074] 1) Removal of genomic DNA
[0075]Add 5×gDNA Eraser Bμffer 2.0μl, gDNA Eraser 1.0μl, total RNA 1μg, add RNase Free ddH in the test tube 2 O to bring the total volume to 10 μl and heat in a water bath at 42°C for 2min.
[0076] 2) Reverse transcription reaction
[0077] Convert 5×Prime Buffer 2 4.0 μl, Prime RT Enzyme Mix I 1.0 μl, RTPrimer Mix 1.0 μl, RNase Free ddH 2 Add 4.0 μl of O to the above test tube and mix together to make a total of 20 μl, place in a water bath at 37°C for 15 minutes, then at 85°C for 5s.
[0078] 4. QPCR amplification detection
[0079] 1) P...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com