Esophageal squamous carcinoma diagnosis and treatment target and application

A technology for esophageal squamous cell carcinoma and reagents, applied in the field of biomedicine, can solve the problems of lack of quantitative standards, cannot exclude the influence of subjective factors, etc., to achieve the effect of improving treatment effect, avoiding insufficient treatment intensity, and meeting detection requirements

Active Publication Date: 2019-06-21
FOURTH HOSPITAL OF HEBEI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no quantitative standard, and the influence of subjective factors cannot be ruled out.

Method used

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  • Esophageal squamous carcinoma diagnosis and treatment target and application
  • Esophageal squamous carcinoma diagnosis and treatment target and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Screening for gene markers associated with esophageal squamous cell carcinoma

[0048] 1. Sample collection

[0049] 57 cases of esophageal squamous cell carcinoma tissues and adjacent tissues were collected, including 17 patients with histological grade I (G1), 21 patients with histological grade II (G2), and histological grade III (G3) 19 patients. Three samples were taken from each group for detection and analysis of gene expression profiles, screening of differentially expressed genes, and verification experiments were performed in all samples of each group.

[0050] 2. Preparation of RNA samples

[0051] Extraction of tissue total RNA using TRIZOL method

[0052] 1) Mince the tissue with scissors, add 1ml Trizol, shake on the shaker for 1min; place at room temperature for 10min to completely decompose the ribosomes.

[0053] 2) Add 200 μl of chloroform (chloroform), close the cap tightly, shake vigorously for 15 seconds, and let stand at room temperat...

Embodiment 2

[0069] Example 2 QPCR sequencing to verify the differential expression of the LINC01322 gene

[0070] 1. Large-scale QPCR verification of the differential expression of the LINC01322 gene.

[0071] 2. The RNA extraction steps are as described in Example 1.

[0072] 3. Reverse transcription:

[0073] The reverse transcription kit (Takara code: DRR047A) of TAKARA Company was used for operation.

[0074] 1) Removal of genomic DNA

[0075]Add 5×gDNA Eraser Bμffer 2.0μl, gDNA Eraser 1.0μl, total RNA 1μg, add RNase Free ddH in the test tube 2 O to bring the total volume to 10 μl and heat in a water bath at 42°C for 2min.

[0076] 2) Reverse transcription reaction

[0077] Convert 5×Prime Buffer 2 4.0 μl, Prime RT Enzyme Mix I 1.0 μl, RTPrimer Mix 1.0 μl, RNase Free ddH 2 Add 4.0 μl of O to the above test tube and mix together to make a total of 20 μl, place in a water bath at 37°C for 15 minutes, then at 85°C for 5s.

[0078] 4. QPCR amplification detection

[0079] 1) P...

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Abstract

The invention discloses esophageal squamous carcinoma diagnosis and treatment target and application. The target is LINC01322. The fact that the LINC01322 has evident differential expression in esophageal squamous carcinoma of different differentiation levels is discovered through the high-throughput sequencing technology and bioinformatics analysis, the fact is further verified through QPCR by increasing sample quantity, and the results indicate that the LINC01322 can be used as a molecular marker for judging esophageal squamous carcinoma hazard level to guide doctors to use different treatment strategies for patients with the esophageal squamous carcinoma at different hazard levels, and medical resources are saved.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a diagnosis and treatment target of esophageal squamous cell carcinoma and its application, and the specific target involved is LINC01322. Background technique [0002] Esophageal cancer is the eighth leading cause of cancer and the sixth leading cause of cancer death in the world (International Agency for Research on Cancer. GLOBOCAN 2012, Estimated Cancer Incidence Mortality and Prevalence Worldwide in 2012. Geneva: World Health Organization, 2012. [C].). Esophageal cancer is the fifth most common cancer and the fourth leading cause of cancer death in China (Zhou MG, Wang Xl, Hu JP, Li GL, Chen WQ, Zhang SW, et al. Geographical distribution of cancer mortality in China, 2004-2005. Chin J Prev Med. 2010;44:303-8.[Z].). The occurrence and development of esophageal cancer is a complex process of multi-factor, multi-stage mutation accumulation and interaction. The activation of oncogenes ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886
Inventor 曹峰朱永刚张璁
Owner FOURTH HOSPITAL OF HEBEI MEDICAL UNIV
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