Bacillus tequilensis CAS-MEI-2-33 and application thereof

A technology of bacillus tequila and bacillus, which is applied in the field of tobacco waste recycling and reuse, and achieves the effects of good temperature stability and stable enzyme activity

Active Publication Date: 2019-07-02
中国烟草总公司海南省公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to expand the existing sources of alkaline pectinase and improve the utilization rate of tobacco waste, the present invention provides a strain of Bacillus tequila CAS-MEI-2-33 and its application, which can use tobacco waste as Fermentation of raw materials to produce pectinase solves the recycling of tobacco waste to a certain extent and expands the source of alkaline pectinase. The technical scheme adopted is as follows:

Method used

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  • Bacillus tequilensis CAS-MEI-2-33 and application thereof
  • Bacillus tequilensis CAS-MEI-2-33 and application thereof
  • Bacillus tequilensis CAS-MEI-2-33 and application thereof

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Embodiment 1

[0023] 1. Materials and methods

[0024] 1. The formula of the culture medium involved in the following examples is as follows:

[0025] (1) LB liquid medium: Yeast extract 4.5-5.5g / L, Trypone 9.5-10.5g / L, NaCl9.5-10.5g / L. Sterilize at 115°C-121°C for 20-30min.

[0026] (2) Solid screening medium: 0.5% pectin, K 2 HPO 4 0.1%, anhydrous magnesium sulfate 0.05%, ammonium sulfate 0.3%, ferrous sulfate 0.001%, agar 1.8%, pH 7.0, sterilized at 121°C for 20min.

[0027] (3) Pectinase fermentation medium:

[0028] Fermentation enzyme production time curve determination medium: tobacco stem 1.5%-2.5%, K 2 HPO 4 0.05%-0.15%, anhydrous magnesium sulfate 0.04-0.06%, ammonium sulfate 0.25%-0.35%, ferrous sulfate 0.001%, natural pH, sterilized at 115°C-121°C for 20-30min.

[0029] Fermentation medium initial pH optimization medium: Tobacco stem 1.5%-2.5%, K 2 HPO 4 0.05%-0.15%, anhydrous magnesium sulfate 0.04-0.06%, ammonium sulfate 0.25%-0.35%, ferrous sulfate 0.001%, using HC...

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Abstract

The invention discloses a strain of Bacillus tequilensis CAS-MEI-2-33 and application thereof, and belongs to the technical field of tobacco waste recycling. The strain is deposited on October 17, 2017 at China General Microbiological Culture Collection Center with a preservation number of CGMCC NO. 14826. The strain has the properties of rapid growth and pectin degradation, and the strain can beused to produce pectinase and obtain a crude pectinase liquid by taking tobacco waste as a raw material for fermentation. The strain can make tobacco waste turn waste into treasure, thereby not only solving the utilization problem of tobacco waste to a certain extent, but also expanding the source of alkaline pectinase. The strain of the invention is suitable for producing pectinase through fermentation.

Description

technical field [0001] The invention relates to a strain of Bacillus tequila CAS-MEI-2-33 and application thereof, and belongs to the technical field of tobacco waste recovery and reuse. Background technique [0002] my country is a big tobacco producing country. In addition to producing a large amount of tobacco leaves every year, a large amount of tobacco waste is produced, including tobacco stems, fireworks, tobacco roots, tobacco branches, axillary buds, top leaves and foot leaves, etc. Discarding it in the field or storing it to rot is not only a waste of resources, but also a kind of damage to the environment. At present, although the utilization and development of tobacco waste is gradually carried out, such as the production of organic fertilizer, the preparation of activated carbon, the cultivation of oyster mushrooms, the preparation of industrial ethanol, and the production of pesticides, etc., due to the limitations of various methods, the degree of development an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/18C12N9/26C12N9/88C12R1/07
CPCC12N1/20C12N9/18C12N9/2408C12N9/88C12Y301/01011C12Y302/01015C12Y402/02002C12N1/205C12R2001/07
Inventor 张海波咸漠张鸽王帆
Owner 中国烟草总公司海南省公司
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