A preparation method and application of magnetic fluorescent coded microspheres

A fluorescent coding and magnetic coding technology, applied in the field of biomedical detection, can solve problems such as environmental hazards

Active Publication Date: 2021-09-21
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

The above polymer microsphere synthesis method is restricted by reaction conditions, including pH value, reaction products, and products that are harmful to the environment

Method used

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  • A preparation method and application of magnetic fluorescent coded microspheres
  • A preparation method and application of magnetic fluorescent coded microspheres
  • A preparation method and application of magnetic fluorescent coded microspheres

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preparation example Construction

[0037] refer to Figure 7 and Figure 8 , in one embodiment, a method for preparing magnetic fluorescently encoded microspheres, comprising the following steps:

[0038] S1. Preparation of unmodified fluorescent magnetically encoded polymer microspheres 4, such as Figure 8 shown;

[0039] S2. By oxidizing the fluorescent magnetically encoded polymer microspheres 4, increasing the carboxyl density on the surface of the polymer microspheres, and changing the surface properties of the polymer microspheres.

[0040] In a preferred embodiment, the polymer microspheres are polyethylene glycol diacrylate (PEGDA) microspheres.

[0041] In a preferred embodiment, ozone is used to oxidize the surface of the polymer microspheres in step S2.

[0042] In a more preferred embodiment, step S2 also includes detecting the change curve of O atoms and C atoms on the surface of the polymer microsphere by X-ray photoelectron spectroscopy analysis, and controlling the reaction time to monitor ...

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Abstract

A method for preparing magnetic fluorescently encoded microspheres and its application, the preparation method comprising the following steps: S1, preparing unmodified fluorescent magnetically encoded polymer microspheres; S2, by oxidizing the polymer microspheres, increasing the The carboxyl group density on the surface of the polymer microspheres is changed to change the surface characteristics of the polymer microspheres. By oxidizing fluorescent magnetically encoded polymer microspheres, the surface of polymer microspheres is modified. When oxidizing, the double bonds on the surface of polymer microspheres are used to generate acid through rearrangement of peroxy radicals, thereby generating carboxyl groups and increasing polymer The density of carboxyl groups on the surface of the microspheres, so that the surface-modified fluorescent magnetically encoded polymer microspheres can efficiently bind a large number of antibody protein molecules, and the antibodies are attached to the surface of the microspheres for in vitro detection. The protein molecule labeled with the marker can realize the corresponding detection function.

Description

technical field [0001] The invention relates to the field of biomedical detection, in particular to a preparation method and application of magnetic fluorescent coded microspheres. Background technique [0002] In the field of biomedical detection, the initially developed solid-phase biochip technology has an important impact on the analysis of high-concentration analytes, but it is limited in reaction speed, quantitative detection and flexibility. In order to solve these problems, a liquid-phase suspension assay based on encoded microspheres to detect analytes was developed, which has the advantages of fast binding kinetics, flexibility of target detection, and high-quality detection results. However, the analysis results of traditional liquid-phase biochips are subject to problems such as difficult operation and expensive later detection equipment. [0003] With the improvement of computer computing power, image detection technology has developed rapidly, and the cost has...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J13/02G01N33/532G01N33/543
CPCB01J13/02G01N33/532G01N33/54326G01N33/54346
Inventor 孙树清姜文博沙洲
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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