A kind of I group 11d type poultry adenovirus strain, inactivated vaccine and preparation method thereof

A technology of avian adenovirus and inactivated vaccine, which is applied in the field of inactivated vaccine and its preparation, group I 11d avian adenovirus strain, virus strain and inactivated vaccine, and can solve the problem that the strain cannot provide good protection and no cross protection. Insufficient prevention and control of vaccines, etc., to achieve the effects of rapid immune response, high safety, and increased virus titer titers

Active Publication Date: 2020-02-14
洛阳职业技术学院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In terms of disease prevention and control, the use of chicken embryos and poultry-derived cells to culture avian adenoviruses can provide certain materials for the research of avian adenovirus vaccines, but because there is no cross-protection between different serotypes of avian adenoviruses, the Serotypes cannot provide good protection against the strains that are prevalent in the market. Therefore, the current market urgently needs to develop a new serotype of avian adenovirus vaccine to make up for the incomplete prevention and control of avian adenovirus vaccines in the market.

Method used

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  • A kind of I group 11d type poultry adenovirus strain, inactivated vaccine and preparation method thereof
  • A kind of I group 11d type poultry adenovirus strain, inactivated vaccine and preparation method thereof
  • A kind of I group 11d type poultry adenovirus strain, inactivated vaccine and preparation method thereof

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Experimental program
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Embodiment 1

[0037] Example 1 Isolation and identification of avian adenovirus RC18 strain

[0038] 1. Epidemiological investigation

[0039] Since 2017, the inventor has discovered a disease characterized by hepatomegaly and hemorrhage from some white-feathered broiler chickens and laying hens in Hebei and other areas of Shandong Province. After clinical investigation and laboratory testing, the initial diagnosis is inclusion bodies caused by group I adenovirus Hepatitis, so the liver, spleen, lung and other diseased tissues of chickens with typical symptoms were collected and stored in a -70°C refrigerator for later use.

[0040] 2. Virus isolation

[0041] After taking the liver of a dead chicken and grinding it up, add sterilized saline at a ratio of 1:5 to make a suspension; after repeated freezing and thawing three times, centrifuge at 12000r / min for 10min, and take the supernatant; add penicillin and streptomycin 10000IU / ml, filtered through a 0.22μm microporous filter, and stored...

Embodiment 2

[0056] Embodiment 2 uses SPF chicken embryo to prepare poultry adenovirus inactivated vaccine

[0057] The method for preparing poultry adenovirus inactivated vaccine according to the present invention covers the following steps: preparation of antigen for making vaccines, inspection of semi-finished products, preparation of vaccine, safety inspection and efficacy inspection, the specific steps are as follows:

[0058] 1. Preparation of antigen solution

[0059] 1.1 Incubation of eggs:

[0060] SPF chicken embryos (purchased from Beijing Meria Weitong Experimental Animal Technology Co., Ltd.) were selected and placed on 42 egg trays, and the air chamber must be placed upwards in the incubator for incubation.

[0061] 1.2 Virus inoculation and harvest:

[0062] The isolated virus species (avian adenovirus RC18) is properly diluted with sterilized physiological saline, press 10 3.0 ~10 4.0 ELD 50 / 0.1ml to inoculate 7-day-old chicken embryos via the chorioallantoic membrane...

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Abstract

The invention discloses an I group 11d serotype fowl adenovirus strain, an inactivated vaccine and a preparation method of the inactivated vaccine. The I group 11d serotype fowl adenovirus strain is named as fowl adenovirus RC18, and the virus strain is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 17404. Experiments prove that the I group 11d serotype fowl adenovirus RC18 strain isolated by the invention has a high titer, strong proliferation ability, good immunogenicity, and matches with epidemic strains. an inactivated vaccine prepared from the I group 11d serotype fowl adenovirus RC18 strain is used to immunize experimental chickens, the experimental chickens can resist infection of the chicken flock caused by clinical epidemic I group fowl adenovirus (11d serotype), the immune response is generated fast, the attack protection rate is high, and no local or systemic adverse reactions caused by the vaccine do not occur, and the safety and stability are good. The invention provides an effective technical means for the prevention and treatment of chicken inclusion body hepatitis.

Description

technical field [0001] The invention relates to a virus strain and an inactivated vaccine, in particular to a group I 11d type poultry adenovirus strain, an inactivated vaccine and a preparation method thereof, belonging to the technical field of veterinary biological products. Background technique [0002] Inclusion body hepatitis (IBH) is an infectious disease caused by group I avian adenovirus (FAdV). The pathogen is fowl adenovirus (Fowl adenovirus, FAdV) serotype 8b, which belongs to Adenoviridae in virus classification, subgroup I fowl adenovirus in the genus of avian adenovirus. [0003] FAdV belongs to the non-enveloped double-stranded DNA virus of the family Adenoviridae (Family Adenoviridae), and can be divided into 12 serotypes, which exhibit very different pathogenicity. Worldwide, avian adenovirus serotypes 2, 3, 8a, 8b, 9 and 11 cause inclusion body hepatitis and, in the presence of serotype 4, pericardial effusion hepatitis syndrome. At present, the main ser...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00A61K39/235A61P31/20C12R1/93
CPCA61K39/12A61K2039/5252A61K2039/552A61P31/20C12N7/00C12N2710/10221C12N2710/10234
Inventor 张贺伟程相朝张春杰田文静宋敏杰张淼丹
Owner 洛阳职业技术学院
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