Construction method of AFFT2 cells

A construction method and cell technology, applied in the field of AFFT2 cells and their preparation, can solve the problem of not considering the self-protection technology of T cells, and achieve the effects of unrestricted killing ability, strong target specificity and high safety.

Active Publication Date: 2019-08-06
BEIJING DCTY BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] None of the above schemes consider the self-protection technology of T cells, so that a small number of specific T cells directly face the powerful tumor microenvironment

Method used

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  • Construction method of AFFT2 cells
  • Construction method of AFFT2 cells
  • Construction method of AFFT2 cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] This example will take lung cancer patients as an example to provide targeted AFFT2 cells and their preparation methods:

[0057] 1. Whole Exome Sequencing

[0058] 1) Take peripheral blood from lung cancer patients for ctDNA sequencing and HLA typing;

[0059] 2) Use software to analyze the sequencing information: compare the ctDNA sequencing results with the genome of normal cells, and screen out the mutation sites;

[0060] 2. Epitope prediction

[0061] 1) Take the mutated amino acid site as the center, extend 10 amino acids to both sides, and use this 21-amino acid polypeptide as a "potential antigenic epitope";

[0062] 2) Use prediction software to analyze the IC50 of the potential antigenic epitope (recommended software: NetMHCpan 3.0, PickPocket, artificial neural networks (ANN)), if IC50<1000nM, the potential antigenic epitope is considered as "antigenic epitope";

[0063] 3. Synthetic peptides

[0064] Entrust a technical service company to synthesize a mut...

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Abstract

The invention belongs to the field of a biological technology, and particularly relates to a construction method of AFFT2 cells. According to the method, the cells are reformed by a TCR-T technique, and the reformed T cells are subjected to in vitro sealing by using inhibition signal molecule antibody medicines, so that the antitumor capacity of the T cells can be effectively improved. For cells reformed by an AFFT2 scheme, the proportion for distinguishing the specific T cells of tumor antigen is 70% or above.

Description

[0001] Technical field: [0002] The invention belongs to the field of biotechnology, and in particular relates to an AFFT2 cell and a preparation method thereof. [0003] Background technique: [0004] At present, in terms of tumor-specific immunotherapy, the existing LAK, DC, CIK, and DC-CIK cells and methods have basically been proven to be ineffective, while NK, CAR-NK, TIL, and other cell technologies have yet to mature. CAR - T cells have shortcomings in safety and solid tumor therapy. [0005] In the prior art, DCs are generally modified to present T cells to produce specific killing. Some laboratories are trying to use viruses as vectors to transfect and present T cells to induce specific killing of T cells. We have also used mutant hybrid peptides to directly stimulate PBMCs to induce T cells. There are also laboratories using TCR-T technology to target the presentation of MAGE A3 antigen. [0006] The above treatment methods are immature, especially the in vitro i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/90C12N15/867C12N5/10
CPCC07K14/7051C12N5/0636C12N15/86C12N15/907C12N2501/51C12N2510/00C12N2740/15043C12N2800/107C12N2810/10
Inventor 焦顺昌张嵘周子珊解佳森陈小彬彭刚陈红利
Owner BEIJING DCTY BIOTECH CO LTD
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