Method for establishing easily aborting animal models

A technology of animal models and model groups, which is applied in animal husbandry and other fields, can solve problems such as unsatisfactory curative effects, achieve good application prospects, reduce modeling time, and achieve high success rates

Inactive Publication Date: 2019-08-09
HUNAN UNIV OF CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The etiology mainly includes chromosomal abnormalities, maternal reproductive tract abnormalities, maternal endocrine abnormalities, immune function abnormalities, reproductive tract infections, cervical insufficiency, and thrombosis tendency, etc. However, 50% of patients with recurrent spontaneous abortions cannot be diagnosed using current clinical diagnostic methods. The cause of repeated miscarriages was discovered, and the curative effect was unsatisfactory. It is called unexplained recurrent spontaneous abortion (URSA), which is a clinically difficult infertility disease.

Method used

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  • Method for establishing easily aborting animal models
  • Method for establishing easily aborting animal models
  • Method for establishing easily aborting animal models

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The present invention shows a method for setting up a miscarriage-prone animal model, the method following steps:

[0037] Step 1, selection of female mice: select healthy 4-week-old female CBA / J mice;

[0038] Step 2. Feeding of female mice: Keep the temperature and humidity constant within a certain range, irradiate with fluorescent lamps for 12 hours, avoid light for 12 hours, replace cages and bedding materials once a week, and feed female CBA / J mice after one week. Divide them into two groups at random; in the second step, the temperature kept constant at 22-25° C., and the humidity range is 40%-75%.

[0039] Step 3, grouping of models: one group of female CBA / J mice was mated with male BALB / c mice at a ratio of 2:1 to establish a normal pregnancy group; another group of female CBA / J mice was mated with male BALB / c mice DBA / 2 mice were mated in cages at a ratio of 2:1 to establish a recurrent abortion model group;

[0040] Step 4. Establishment of the model: fema...

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Abstract

The invention discloses a method for establishing easily aborting animal models. The method includes the following steps of firstly, selecting female mice; secondly, feeding the female mice; thirdly,conducting model grouping; fourthly, conducting model establishment, wherein female CBA / J mice are subjected to vaginal smearing in the next morning of cohabitation, the day when a large number of sperms appear on vaginal smearing slides and mice's vaginal plugs can be seen is regarded as the 0th day, female CBA / J mice of the recurrent abortion model group are subjected to back subcutaneous injection with epinephrine hydrochloride on the 14th day of gestation and immersed in water to swim, and back subcutaneous injection with epinephrine hydrochloride is repeated; fifthly, judging whether or not the establishment of the aborting animal models succeeds or not. The method for establishing the animal models is safe, reliable, simple and economical, the modeling time of the mouse models is shortened, the modeling success rate is increased, and the modeling efficiency is improved, so that the method has good application prospects.

Description

technical field [0001] The invention relates to the technical field of establishment of abortion animal models, in particular to a method for establishment of abortion-prone animal models. Background technique [0002] Recurrent spontaneous abortion (RSA) is one of the common pathological pregnancies in obstetrics and gynecology, which seriously threatens human reproductive health. The etiology mainly includes chromosomal abnormalities, maternal reproductive tract abnormalities, maternal endocrine abnormalities, immune function abnormalities, reproductive tract infections, cervical insufficiency, and thrombosis tendency, etc. However, 50% of patients with recurrent spontaneous abortions cannot be diagnosed using current clinical diagnostic methods. If the cause of recurrent miscarriage is discovered and the curative effect is not satisfactory, it is called unexplained recurrent spontaneous abortion (URSA), which is a clinically difficult infertility disease. Therefore, expl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 杨冬梅雷磊逯晶邹芳张小欢
Owner HUNAN UNIV OF CHINESE MEDICINE
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