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Label-free biosensing method based on refractive index modulation time response

A technology of refractive index modulation and refractive index sensor, which is applied in phase influence characteristic measurement, instruments, measuring devices, etc., can solve the problems of small size, high quality requirements of personnel, and inability to realize specific sensing, etc., and simplify the difficulty of development , The experimental operation is simple, and the effect of high precision is guaranteed

Active Publication Date: 2019-08-13
中物慧芯信息科技(苏州)有限公司
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, the functionalization of the surface of optical devices requires the operation of professionals, which requires high quality personnel
In addition, due to the extremely small size of these optical devices, after functionalization of the device surface and biosensing experiments, the optical devices are difficult to clean and reuse, making it difficult to ensure the reproducibility of biosensing
[0004] Although there is a literature "S.Lin, et.al., ACS Nano, 2013, 7(2):1725–1730" reported a label-free biosensor assisted by optical gradient force adsorption, which does not require functionalization of the surface of the optical device , but the optical gradient force is not selective for the adsorption of biomolecules, and cannot achieve specific sensing

Method used

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  • Label-free biosensing method based on refractive index modulation time response
  • Label-free biosensing method based on refractive index modulation time response
  • Label-free biosensing method based on refractive index modulation time response

Examples

Experimental program
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Embodiment 1

[0032] like Figure 1-2 As shown, the label-free biosensing method based on the refractive index modulation time response of the present invention comprises the following steps:

[0033] S1: Molecular probes are coupled to the surface of magnetic nanoparticles in magnetic fluid to prepare functionalized magnetic nanoparticles, and the molecular probes have specific recognition ability;

[0034] S2: Add the biomolecules to be tested into the functionalized magnetic fluid. If the biomolecules to be tested meet the specific detection conditions, the biomolecules to be tested will react with the molecular probes on the surface of the magnetic nanoparticles, and induce the self-assembly of the magnetic nanoparticles to form a volume Larger magnetic nanoparticle clusters, the magnetic fluid after self-assembly of functionalized magnetic nanoparticles induced by the biomolecules to be tested becomes a self-assembled magnetic fluid;

[0035] S3: Apply or remove a constant modulation ...

Embodiment 2

[0043] like image 3 As shown, on the basis of Example 1, the refractive index modulation time response process of the self-assembled magnetic fluid under the action of a modulated magnetic field is measured by using a refractive index sensor, taking a refractive index sensor based on an interferometer as an example, but it is not limited to this principle All sensors capable of measuring the refractive index of liquids are applicable to the present invention.

[0044] Include the following steps:

[0045] S11: Add the self-assembled magnetic fluid to the sensing arm of the refractive index sensor, and record the interference fringe curve of the refractive index sensor;

[0046] S12: Apply a modulated magnetic field to the self-assembled magnetic fluid, and the refractive index of the self-assembled magnetic fluid will gradually change as the time of the magnetic field increases;

[0047] S13: Record the interference fringe curve of the refractive index sensor, and obtain th...

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Abstract

The invention discloses a label-free biosensing method based on a refractive index modulation time response, and belongs to the technical field of label-free biosensors. According to the method, functional magnetic nanoparticles in biomolecule induced magnetic fluid to be detected are self-assembled, a magnetic nanoparticle cluster with a large volume is generated, and the refractive index modulation time response of the magnetic fluid is changed when the modulation magnetic field is applied or removed. Under the action of the same modulation magnetic field, the biomolecules with different concentrations enable the magnetic fluid to generate different refractive index modulation time responses. The refractive index sensor is used for measuring a refractive index modulation time response process of the self-assembled magnetic fluid under the action of an external magnetic field, so that the detection of biomolecules can be realized. The refractive index sensor does not need to be functionalized, the detection process is greatly simplified, and the problems in the prior art are solved.

Description

technical field [0001] The invention relates to a label-free biosensing method based on refractive index modulation time response, and belongs to the technical field of label-free biosensors. Background technique [0002] According to the classification of whether to use markers, biosensors can be divided into two types: labeled type and label-free type. Labeled biosensors must use labeled indicator reagents and use sophisticated equipment to detect the markers. The sensing system is expensive and requires professionals to operate. The label-free biosensor does not need to label the biological information to be measured, and detects the biomass by directly detecting the physical and chemical changes formed by the biological reaction. The detection is carried out in the original form of the biological sample without changing the characteristics of the biological sample, which greatly simplifies the operation process. Therefore, label-free biosensors have attracted the atten...

Claims

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Application Information

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IPC IPC(8): G01N21/17G01N21/41
CPCG01N21/1717G01N21/41G01N2021/1727
Inventor 马任德李晓平曹洪忠满忠晓夏云杰
Owner 中物慧芯信息科技(苏州)有限公司
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