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Label-free biosensing method based on refractive index modulation time response

A technology of refractive index modulation and time response, applied in the measurement of phase influence characteristics, instruments, measuring devices, etc., can solve the problems of small size, high personnel quality requirements, inability to achieve specific sensing, etc., to simplify the development difficulty, Simple experimental operation and high-precision effect

Active Publication Date: 2021-11-30
中物慧芯信息科技(苏州)有限公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, the functionalization of the surface of optical devices requires the operation of professionals, which requires high quality personnel
In addition, due to the extremely small size of these optical devices, after functionalization of the device surface and biosensing experiments, the optical devices are difficult to clean and reuse, making it difficult to ensure the reproducibility of biosensing
[0004] Although there is a literature "S.Lin, et.al., ACS Nano, 2013, 7(2):1725–1730" reported a label-free biosensor assisted by optical gradient force adsorption, which does not require functionalization of the surface of the optical device , but the optical gradient force is not selective for the adsorption of biomolecules, and cannot achieve specific sensing

Method used

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  • Label-free biosensing method based on refractive index modulation time response
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  • Label-free biosensing method based on refractive index modulation time response

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Embodiment 1

[0032] Such as Figure 1-2 As shown, the label-free biosensing method based on the refractive index modulation time response of the present invention comprises the following steps:

[0033] S1: Molecular probes are coupled to the surface of magnetic nanoparticles in magnetic fluid to prepare functionalized magnetic nanoparticles, and the molecular probes have specific recognition ability;

[0034] S2: Add the biomolecules to be tested into the functionalized magnetic fluid. If the biomolecules to be tested meet the specific detection conditions, the biomolecules to be tested will react with the molecular probes on the surface of the magnetic nanoparticles, and induce the self-assembly of the magnetic nanoparticles to form a volume Larger magnetic nanoparticle clusters, the magnetic fluid after self-assembly of functionalized magnetic nanoparticles induced by the biomolecules to be tested becomes a self-assembled magnetic fluid;

[0035] S3: Apply or remove a constant modulati...

Embodiment 2

[0043] Such as image 3 As shown, on the basis of Example 1, the refractive index modulation time response process of the self-assembled magnetic fluid under the action of a modulated magnetic field is measured by using a refractive index sensor, taking a refractive index sensor based on an interferometer as an example, but it is not limited to this principle All sensors capable of measuring the refractive index of liquids are applicable to the present invention.

[0044] Include the following steps:

[0045] S11: Add the self-assembled magnetic fluid to the sensing arm of the refractive index sensor, and record the interference fringe curve of the refractive index sensor;

[0046] S12: Apply a modulated magnetic field to the self-assembled magnetic fluid, and the refractive index of the self-assembled magnetic fluid will gradually change as the time of the magnetic field increases;

[0047] S13: Record the interference fringe curve of the refractive index sensor, and obtain...

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Abstract

The invention discloses a label-free biosensing method based on refractive index modulation time response, which belongs to the technical field of label-free biosensors. Biomolecules to be tested induce the self-assembly of functionalized magnetic nanoparticles in a magnetic fluid to produce a larger volume of magnetic Nanoparticle clusters that alter the temporal response of a ferrofluid's refractive index modulation when a modulating magnetic field is applied or removed. Under the action of the same modulating magnetic field, different concentrations of biomolecules make the magnetic fluid produce different time responses of refractive index modulation. The detection of biomolecules can be realized by using a refractive index sensor to measure the time response process of the self-assembled ferrofluid under the action of an external magnetic field. There is no need to functionalize the refractive index sensor, the detection process is greatly simplified, and the problems in the prior art are solved.

Description

technical field [0001] The invention relates to a label-free biosensing method based on refractive index modulation time response, and belongs to the technical field of label-free biosensors. Background technique [0002] According to the classification of whether to use markers, biosensors can be divided into two types: labeled type and label-free type. Labeled biosensors must use labeled indicator reagents and use sophisticated equipment to detect the markers. The sensing system is expensive and requires professionals to operate. The label-free biosensor does not need to label the biological information to be measured, and detects the biomass by directly detecting the physical and chemical changes formed by the biological reaction. The detection is carried out in the original form of the biological sample without changing the characteristics of the biological sample, which greatly simplifies the operation process. Therefore, label-free biosensors have attracted the atten...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/17G01N21/41
CPCG01N21/1717G01N21/41G01N2021/1727
Inventor 马任德李晓平曹洪忠满忠晓夏云杰
Owner 中物慧芯信息科技(苏州)有限公司
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