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Induction method for haliotis discus hanai triploid

A technique for triploid and triploid abalone is applied in the field of triploid induction of abalone, which can solve the problems of low induction rate and poor stability, and achieve the effect of optimizing the induction conditions.

Active Publication Date: 2019-08-30
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve the problems of low induction rate and poor stability in the existing hybridization technology, and provide a method for inducing an efficient and stable triploid of abalone wrinkle

Method used

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  • Induction method for haliotis discus hanai triploid

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1) Take the wrinkled plate abalone with good individual vitality and no external damage as the seed abalone. The average shell length of the wrinkled plate abalone is 76.09±9.57mm, the shell width is 50.66±4.19mm, and the wet weight is 55.50±13.88g. Place them in different parent abalone breeding ponds for breeding, and the sex characteristics of the parent abalones in each parent abalone cultivation pond must be single.

[0032] 2) After the abalone gonads mature, dry them in the shade for 60 minutes, then place the male and female individuals in different clearly marked containers, inject ultraviolet-treated seawater with an irradiation intensity of 1000mWh / L, the seawater temperature is 22°C, and replace the ultraviolet treatment every 60min Seawater, until the gametes are discharged, the operation process strictly prevents sperm and eggs from contacting.

[0033] 3) Use a 150 μm sieve to filter out the impurities in the sperm and eggs. Microscopically check the qual...

Embodiment 2

[0040] 1) Take the wrinkled plate abalone with good individual vitality and no external damage as the seed abalone. The average shell length of the wrinkled plate abalone is 72.94±3.31mm, the shell width is 48.73±3.50mm, and the wet weight is 50.93±9.10g. Place them in different parent abalone breeding ponds for breeding, and the sex characteristics of the parent abalones in each parent abalone cultivation pond must be single.

[0041] 2) After the abalone gonads mature, dry them in the shade for 60 minutes, then place the male and female individuals in different clearly marked containers, inject ultraviolet-treated seawater with an irradiation intensity of 900mWh / L, and the seawater temperature is 22°C, and replace the ultraviolet treatment every 60min Seawater, until the gametes are discharged, the operation process strictly prevents sperm and eggs from contacting.

[0042] 3) Use a 150 μm sieve to filter out the impurities in the sperm and eggs, and confirm the egg quality ...

Embodiment 3

[0049] 1) Take the wrinkled plate abalone with good individual vitality and no external damage as the seed abalone. The average shell length of the wrinkled plate abalone is 76.61±5.06mm, the shell width is 51.95±2.88mm, and the wet weight is 54.43±7.57g. Place them in different parent abalone breeding ponds for breeding, and the sex characteristics of the parent abalones in each parent abalone cultivation pond must be single.

[0050] 2) After the abalone gonads mature, dry them in the shade for 60 minutes, then place the male and female individuals in different clearly marked containers, inject ultraviolet-treated seawater with an irradiation intensity of 1000mWh / L, and the seawater temperature is 21°C, and replace the ultraviolet treatment every 60min Seawater, until the gametes are discharged, the operation process strictly prevents sperm and eggs from contacting.

[0051] 3) Use a 150 μm sieve to filter out the impurities in the sperm and eggs, and confirm the egg quality...

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Abstract

The invention discloses an induction method for a haliotis discus hanai triploid, and relates to a shellfish culture method. The method comprises the steps of selecting abalone seeds and promoting maturity; performing artificial spawning induction; selecting and processing gametes; performing synchronous fertilization; performing medicine induction; removing medicine; performing incubation. On thepremise of ensuring haliotis discus hanai seed gonad maturity and synchronous fertilization, the induction condition is further optimized, and therefore the haliotis discus hanai triploid is obtainedefficiently and stably. Synchronous fertilization is ensured, the phenomenon that gametes are in contact in advance in the discharge process is strictly avoided, microscopic examinations determine that no accident fertilization exists, and the consistency of synchronous fertilization and germ cell development is ensured. The induction conditions are optimized, wherein constant microscopic examination observation determines that 70-80% of contrast group germ cells PB1 are adopted as a processing time node, it is ensured that a first polar body is effectively released, and tetraploids and aneuploids are avoided. According to the stable and efficient induction rate, the induction rate of the triploid is 100% or is close to 100%.

Description

technical field [0001] The invention relates to a method for cultivating shellfish, in particular to a method for inducing triploid of abalone wrinkled. Background technique [0002] Abalone is a rare marine treasure, known as the first of the "Eight Treasures of Seafood". As the world's largest abalone-raising country, my country's total abalone production in 2017 was 148,500 tons, with an annual output value of over 20 billion yuan. At present, the main species of abalone in my country is Haliotis discus hannai, and the aquaculture range extends from the Yellow Sea and the Bohai Sea in the north of my country to the coast of Fujian. The aquaculture output in Fujian Province reached 123,400 tons (2017), accounting for the total abalone in the country. 83.1% of the yield. However, in the sea area of ​​Fujian, autumn is the peak breeding season of Abalone rugosa. At this time, the gonads of Abalone rugosa cultured in the sea area are fully developed, and sperm eggs are easil...

Claims

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Application Information

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IPC IPC(8): A01K67/033
CPCA01K67/0334
Inventor 王怡骆轩游伟伟柯才焕王星泽
Owner XIAMEN UNIV
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