Blood cell analysis method and blood cell analyzer

Abstract

The invention relates to a blood cell analysis method and a blood cell analyzer. In the method and the instrument, information representing white blood cell fragments is obtained according to side scattered light information and fluorescence information of blood cells, information representing platelets is obtained according to forward scattered light information and fluorescence information, and then the information representing platelets and the information representing white blood cell fragments are obtained. Obtaining the platelet count value can avoid the influence of white blood cell fragments on platelet count, and ensure the accuracy of platelet count without increasing the cost.

Description

technical field

[0001] The invention relates to the field of medical equipment, in particular to a blood cell analysis method and a blood cell analyzer. Background technique

[0002] In recent years, blood cell analyzers that use flow cytometry to count and classify blood cells have come out one after another. Traditional blood cell analyzers use Coulter's principle (also known as resistance method) to count platelets, but are susceptible to the influence of small red blood cells and red blood cell fragments. In recent years, the method of counting platelets using fluorescent dyeing and laser scattering, also known as platelet optical counting method, has emerged. The principle is to generate photoelectric signals through laser irradiation on cells treated with fluorescent reagents. Different photoelectric signals can reflect the physical and chemical characteristics of cells, such as cell size, granularity, and expression of antigen molecules, so that different types of ce...

Examples

Embodiment 1

[0042] Provide the first blood sample containing white blood cell fragments, process the first blood sample to spheroidize the red blood cells, and basically maintain the integrity of the red blood cells, let the cells pass through the detection area of ​​the blood cell analyzer one by one, and detect the precursor of each cell There are three signals: forward scattered light, side scattered light and fluorescence. in figure 2 showing the forward scattered light-fluorescence scattergram (first scattergram) of the first blood sample, image 3 A side scattered light-fluorescence scattergram (second scattergram) of the first blood sample is shown. figure 2 In , the distinction between platelets and white blood cell fragments is poor, so platelet counts cannot be obtained accurately. exist image 3 Among them, platelets and white blood cell fragments can be distinguished, but platelets and red blood cells are not well distinguished, so you can use image 3 Calculate the whit...

Embodiment 2

[0046] Provide a second blood sample containing white blood cell fragments, process the second blood sample to spheroidize red blood cells and maintain the integrity of red blood cells, let the cells pass through the detection area of ​​the blood cell analyzer one by one, and detect the forward direction of each cell Scattered light, side scattered light and fluorescence three signals. in Figure 5 shows the forward scattered light-fluorescence scatter diagram of the second blood sample, Image 6 A side scatter-fluorescence scatter plot of the second blood sample is shown. Figure 5 In , the distinction between platelets and white blood cell fragments is poor, so platelet counts cannot be obtained accurately. exist Image 6 In , platelets and white blood cell fragments can be distinguished, but platelets and red blood cells are not distinguished, so they can be distinguished in Image 6 Determine the preset feature area in the preset feature area, and identify the scattere...

technical field

[0001] The invention relates to the field of medical equipment, in particular to a blood cell analysis method and a blood cell analyzer. Background technique

[0002] In recent years, blood cell analyzers that use flow cytometry to count and classify blood cells have come out one after another. Traditional blood cell analyzers use Coulter's principle (also known as resistance method) to count platelets, but are susceptible to the influence of small red blood cells and red blood cell fragments. In recent years, the method of counting platelets using fluorescent dyeing and laser scattering, also known as platelet optical counting method, has emerged. The principle is to generate photoelectric signals through laser irradiation on cells treated with fluorescent reagents. Different photoelectric signals can reflect the physical and chemical characteristics of cells, such as cell size, granularity, and expression of antigen molecules, so that different types of ce...

Examples

Embodiment 1

[0042] Provide the first blood sample containing white blood cell fragments, process the first blood sample to spheroidize the red blood cells, and basically maintain the integrity of the red blood cells, let the cells pass through the detection area of ​​the blood cell analyzer one by one, and detect the precursor of each cell There are three signals: forward scattered light, side scattered light and fluorescence. in figure 2 showing the forward scattered light-fluorescence scattergram (first scattergram) of the first blood sample, image 3 A side scattered light-fluorescence scattergram (second scattergram) of the first blood sample is shown. figure 2 In , the distinction between platelets and white blood cell fragments is poor, so platelet counts cannot be obtained accurately. exist image 3 Among them, platelets and white blood cell fragments can be distinguished, but platelets and red blood cells are not well distinguished, so you can use image 3 Calculate the whit...

Embodiment 2

[0046] Provide a second blood sample containing white blood cell fragments, process the second blood sample to spheroidize red blood cells and maintain the integrity of red blood cells, let the cells pass through the detection area of ​​the blood cell analyzer one by one, and detect the forward direction of each cell Scattered light, side scattered light and fluorescence three signals. in Figure 5 shows the forward scattered light-fluorescence scatter diagram of the second blood sample, Image 6 A side scatter-fluorescence scatter plot of the second blood sample is shown. Figure 5 In , the distinction between platelets and white blood cell fragments is poor, so platelet counts cannot be obtained accurately. exist Image 6 In , platelets and white blood cell fragments can be distinguished, but platelets and red blood cells are not distinguished, so they can be distinguished in Image 6 Determine the preset feature area in the preset feature area, and identify the scattere...

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