Application of arctigenin in preparation of drug for specifically killing adriamycin-resistant liver cancer cells

A technology of arctigenin and doxorubicin resistance, which is applied in the application field of medicine, can solve the problems of poor treatment effect of liver cancer cells, and achieve the effect of clear composition structure, easy research and strong activity

Inactive Publication Date: 2019-09-20
GUANGDONG MEDICAL UNIV
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Problems solved by technology

[0005] In order to overcome the defect of poor therapeutic effect of the existing drug-resistant liver cancer cells, the present invention provides the application of arctigenin in the preparation of drugs that specifically kill doxorubicin-resistant liver cancer cells. Arctigenin can specifically kill drug-resistant liver cancer cells. Doxorubicin-resistant liver cancer cells are friendly to normal liver cancer cells and are ideal drugs for the treatment of doxorubicin-resistant liver cancer cells

Method used

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  • Application of arctigenin in preparation of drug for specifically killing adriamycin-resistant liver cancer cells
  • Application of arctigenin in preparation of drug for specifically killing adriamycin-resistant liver cancer cells
  • Application of arctigenin in preparation of drug for specifically killing adriamycin-resistant liver cancer cells

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Experimental program
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Embodiment 1

[0026] In this experiment, the CCK-8 method (Cell Counting Kit-8) was used to detect the effect of arctigenin on cell proliferation and toxicity. The principle is as follows:

[0027] Such as figure 1 As shown, the reagent contains WST-8 [chemical name: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-di Sulfonic acid phenyl)-2H-tetrazole monosodium salt], it is dehydrogenase in the cell under the effect of electron carrier 1-methoxy-5-methylphenazinium sulfate (1-MethoxyPMS) It is reduced to a highly water-soluble yellow formazan product (Formazan dye). The amount of formazan produced is directly proportional to the number of viable cells and inversely proportional to cytotoxicity. Use a microplate reader to detect the OD value at a wavelength of 450nm, and indirectly reflect the number of living cells according to the OD value. This property can therefore be exploited for cell proliferation and toxicity assays.

[0028] The specific operation of this experiment is as...

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Abstract

The invention provides application of arctigenin in preparation of drug for specifically killing adriamycin-resistant liver cancer cells, and relates to the technical field of cancer medicines. Experimental results show that compared with other 74 traditional Chinese medicine monomers, the arctigenin can specifically kill adriamycin-resistant liver cancer cells HepG2 (R+ HepG2), and does not have obvious killing effect on HepG2 cells and other types of tumor cells. Moreover, the arctigenin has no effect on immortalized human normal hepatocytes LO-2 at the same drug concentration. The results show that the arctigenin is a kind of liver-friendly anticancer traditional Chinese medicine monomer.

Description

technical field [0001] The invention relates to the technical field of cancer medicines, in particular to the application of arctigenin in the preparation of medicines for specifically killing doxorubicin-resistant liver cancer cells. Background technique [0002] Primary liver cancer (hepatocellular carcinoma (HCC)) is the sixth most common cancer and the second most common cause of cancer mortality worldwide. There are currently a variety of therapies for the treatment of HCC clinically, but they are not universally applicable to the treatment of all patients. Surgical resection is currently the main measure for the clinical treatment of HCC. Although the overall survival rate of patients treated with liver resection increases, most patients relapse HCC within the first 2 years after resection. And because of various factors such as tumor size, location, or poor liver function, many HCCs are not suitable for surgical resection clinically, so drug treatment occupies a very...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/365A61P35/00
CPCA61K31/365A61P35/00
Inventor 何志巍
Owner GUANGDONG MEDICAL UNIV
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