Photothermal therapy and chemical therapy combined targeted medicine delivery system and synthesis method

A chemotherapy and drug delivery system technology, applied in the medical field, can solve the problems of limited curative effect, poor targeting of head and neck squamous cell carcinoma, and the combination of photothermal therapy and chemotherapy, so as to reduce damage, improve therapeutic effect, and be well received The effect of cellular uptake

Inactive Publication Date: 2019-09-24
SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0002] Traditional photothermal therapy is applied to deep tumor tissue, because the tumor tissue is deep, and the curative effect is limited. Oral head and neck tumors, because the tumor tissue is superficial, can have a better effect, but most drug delivery systems target head and neck squamous cell carcinoma. Poor, not combining photothermal therapy with chemotherapy

Method used

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  • Photothermal therapy and chemical therapy combined targeted medicine delivery system and synthesis method
  • Photothermal therapy and chemical therapy combined targeted medicine delivery system and synthesis method
  • Photothermal therapy and chemical therapy combined targeted medicine delivery system and synthesis method

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preparation example Construction

[0036] Such as figure 1 As shown, according to an embodiment of the present invention, a synthesis method for targeted drug delivery combining photothermal and chemotherapy is also provided, including the following steps:

[0037] S101, combined with doxorubicin:

[0038] Take 1 mL of PEGylated AuNPs (2.46 mg / mL), centrifuge at 15,000 rpm for 10 min, remove the supernatant, add EDC / NHS solution and shake for 2 h, after washing, add doxorubicin (0.1 mg / mL) in PBS solution, shake overnight, PEG end The carboxyl group of doxorubicin reacts with the amino group of doxorubicin to form a DOX-AuNPs complex;

[0039] S103, Conjugate Podoplanin Antibody:

[0040] Add DOX-AuNPs complex to EDC / NHS solution and shake at 37°C for 2 hours. After washing, add 40 μL Podoplanin antibody, ultrasonically shake for 10 seconds, shake for 7 hours, wash with PBS, add 5% BSA+0.05% Tween in PBS solution and shake overnight to synthesize Podoplanin- AuNPs-DOX complexes.

[0041] Further, both the E...

specific Embodiment approach ： Embodiment 1

[0043] Specific embodiments: Example 1: drug release detection

[0044] The synthetic material Podoplanin-AuNPs-DOX was dissolved in 1 mL of LPBS and sealed in a dialysis bag. The dialysis bag was placed in a 10 mL medium, shaken at 37°C and 120 rpm, and the release of DOX (doxorubicin) was recorded at different times.

Embodiment 2

[0045] Example 2: 293T cytotoxicity detection

[0046] The non-tumor cell 293T was selected to detect the toxicity of PEGylated gold nanoparticles (hereinafter referred to as AuNPs). The 293T cells were seeded in a 96-well plate, and 5×103 cells were added to each well. Cultured in DMEM+10% fetal bovine serum+1% penicillin-streptomycin medium for 24 hours, then sucked off the medium, washed 3 times with PBS, added / mL, 200 μL / well) culture medium of AuNPs for 48h, and then use the MTT kit to test the cell activity (cell proliferation and cytotoxicity detection kit). After AuNPs were treated for 48 hours, the cell viability was still high, which proved that AuNPs had low toxicity.

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Abstract

The invention belongs to the technical field of medicines, and particularly relates to a photothermal therapy and chemical therapy combined targeted medicine delivery system which comprises a targeted functional molecule, a chemotherapy medicine and a nano-carrier. The targeted functional molecule is an active targeted antibody. The chemotherapy medicine is doxorubicin. The nano-carrier is a pegylated nanometer golden ball with a photothermal effect. The chemotherapy medicine is connected to the pegylated nanometer golden ball through a chemical bound and the antibody. The targeted medicine delivery system has the advantages that the medicine-carrying system has the targeting property, low toxicity and good cell intake, the high-efficiency anti-tumor property can be realized through the combination of photothermal therapy and chemical therapy, the gathering on the oral cavity, head and neck tumor tissue is increased, the damage of chemotherapy medicines to other normal tissue is reduced, and the tumor treatment effect is remarkably improved.

Description

technical field [0001] The invention belongs to the field of medical technology, and in particular relates to a targeted drug delivery system and a synthesis method combining photothermal and chemical therapy. Background technique [0002] Traditional photothermal therapy is applied to deep tumor tissue, because the tumor tissue is deep, and the curative effect is limited. Oral head and neck tumors, because the tumor tissue is superficial, can have a better effect, but most drug delivery systems target head and neck squamous cell carcinoma. Poor sex, did not combine photothermal therapy with chemotherapy. Contents of the invention [0003] Aiming at the above problems, the present invention provides a targeted drug delivery system and synthesis method combining photothermal and chemotherapy. [0004] According to one aspect of the present invention, a targeted drug delivery system combining photothermal and chemotherapy is provided, including targeting functional molecule...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/68A61K47/60A61K41/00A61K31/704A61P35/00
CPCA61K47/6851A61K47/6809A61K47/60A61K41/0052A61K31/704A61P35/00A61K2300/00
Inventor 徐骎刘增赢石剑波鲍欣陈万涛
Owner SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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