A method for identifying the mesocotyl elongation ability of dry-seeded rice

An identification method, dry direct seeding technology, applied in rice cultivation, botany equipment and methods, agriculture, etc., can solve the problems of poor repeatability of experimental results, achieve the effects of shortening the test cycle, improving identification efficiency, and clear formula

Active Publication Date: 2021-04-13
AGRI GENOME INST OF SHENZHEN CHINESE ACADEMY OF AGRI SCI
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

However, many QTLs controlling mesocotyl elongation have been mapped in different studies so far, but due to the significant interaction between mesocotyl elongation and environmental conditions, the reproducibility of the experimental results is poor

Method used

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  • A method for identifying the mesocotyl elongation ability of dry-seeded rice
  • A method for identifying the mesocotyl elongation ability of dry-seeded rice
  • A method for identifying the mesocotyl elongation ability of dry-seeded rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The mesocotyls of the same rice population (208 rice germplasms introduced by International Rice) were identified by hydroponics, sand culture, soil culture (field loam) and nutrient soil (Germany K brand-TS1) culture methods elongation ability. Two independent repetitions were set up for each culture method. The specifications of the hole plates used are 54cm (length) × 28cm (width) × 11cm (height), 50 holes.

[0021] a. In the hydroponics method, spread gauze on the bottom of each hole, select 15 full seeds and spread them on the gauze, place the hole tray in the tray and fill the tray with water until the water surface is less than 1cm above the seeds Then use tinfoil paper to seal the plugs and trays and shield them from light. Finally, place the sealed plugs in an artificial climate chamber at 30°C. After culturing for 7 days, use a ruler to measure the mesocotyl length.

[0022] b. Sand culture method: Before sowing, fill each hole in the hole with sand to a dis...

Embodiment 2

[0030] The variation of mesocotyl length in 395 MAGIC rice populations was detected by the nutrient soil culture method, and the specific operation steps were the same as the above-mentioned nutrient soil culture method.

[0031] Analysis of test results:

[0032] The variation range of mesocotyl length in MAGIC population was 0–4.8 cm, and the distribution was uniform and continuous, which was basically consistent in the two experimental repetitions ( image 3 ). At the same time, the mesocotyl length was highly correlated in two experimental replicates (R 2 =0.8459)( Figure 4 ), basically consistent with the correlation results in Example 11.

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Abstract

The invention discloses a method for identifying the elongation ability of mesocotyl of dry-directed rice, which adopts a nutrient soil culture method under high temperature (35°C, RH=50%-60%) and low humidity conditions to identify the elongation of rice mesocotyl ability, observe the emergence of seedlings every day, record the sequence of emergence of different varieties, and measure the mesocotyl length on the third day after the emergence of the first variety; take the seedlings out of the plugs in order, rinse them, and place them on the On a flat plate with a scale, and take pictures; after all varieties are photographed in turn, use Image J software to set the scale according to the scale on the plate to measure the mesocotyl length of each variety. Through the application of the present invention in the identification of the mesocotyl elongation ability of dry direct-seeding rice, the test period can be significantly shortened, the efficiency of mesocotyl phenotype identification can be improved, and the test error caused by human factors can be reduced. At the same time, the reproducibility and stability of the identification results of mesocotyl elongation ability of dry-seeded rice are greatly improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for identifying the mesocotyl elongation ability of dry-directed rice. , Background technique [0002] Dry direct-seeding rice has a series of advantages such as water saving, labor saving, and suitable for mechanized cultivation. However, in the production of dry direct-seeding rice, due to insufficient rooting depth, lodging phenomenon often occurs in the later stage of rice growth. To solve this problem, in In production, it is often necessary to increase the sowing depth. Deep sowing is conducive to the rooting of rice, improving the water and nutrient absorption capacity of the root system, and improving the lodging resistance of rice. Insufficient seedlings and weed control problems. And under the condition of deep sowing, the seed germination lasts for a long time, during which the seedlings cannot carry out photosynthesis, which will easily cause the growth of the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G22/22A01G31/00
CPCA01G31/00A01G22/22
Inventor 刘宏岩詹俊辉叶国友
Owner AGRI GENOME INST OF SHENZHEN CHINESE ACADEMY OF AGRI SCI
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