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Application of fluoxetine in anti-mycobacterium tuberculosis drug

A technology of Mycobacterium tuberculosis and Mycobacterium tuberculosis, which is applied in the field of bioengineering, can solve the problems of reducing the affinity of acetylcholine and norepinephrine receptors, etc., and achieve the effect of enhancing cell killing of Mycobacterium tuberculosis and improving the development value.

Inactive Publication Date: 2019-10-18
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

SSRIs have significantly lower affinity for histamine, acetylcholine, and norepinephrine receptors than tricyclic antidepressants

Method used

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  • Application of fluoxetine in anti-mycobacterium tuberculosis drug
  • Application of fluoxetine in anti-mycobacterium tuberculosis drug
  • Application of fluoxetine in anti-mycobacterium tuberculosis drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Differentiation of U937 macrophages (No. BNCC100967): Suspended U937 macrophages were cultured in RPMI-1640 medium containing 10% FBS, and placed at a temperature of 37°C, containing 5% CO 2 Cultured in a cell incubator, added PMA with a final concentration of 20ng / ml, cultured overnight, differentiated into macrophages, washed twice with PBS, digested with trypsin, resuspended, added to a 96-well cell culture plate , so that the number of cells per well is 1x104;

[0022] 2. Determination of cell survival rate: After the above-mentioned U937 macrophages were cultured for 24 hours, different concentrations of drugs were added to make the final drug concentrations 10uM, 1uM, and 0.1uM. The experiment was repeated three times for each concentration, and DMSO was used as a parallel control In the experiment, DMSO with the same volume as the drug was added to the control group, and the experiment was repeated three times. After 48 hours, 10ul WST-1 solution was added to ...

Embodiment 2

[0028] 1. Differentiation of U937 macrophages: Suspended U937 macrophages were cultured in RPMI-1640 medium containing 10% FBS, and placed at a temperature of 37°C, containing 5% CO 2 Cultured in a cell incubator, added PMA with a final concentration of 20ng / ml, cultured overnight, differentiated into macrophages, washed twice with PBS, digested with trypsin, resuspended, added to a 96-well cell culture plate , so that the number of cells per well is 1x104;

[0029] 2. Determination of intracellular bactericidal activity: After the above-mentioned U937 macrophages were cultured for 24 hours, fluoxetine was added to make the final concentration 1uM, and the experiment was repeated 3 times for each concentration. DMSO was used as a parallel control experiment. In the control group, fluoxetine was added with DMSO with the same drug volume was repeated three times. After 4 hours, the medium was sucked off, washed twice with PBS, fresh medium was added for 72 hours, and the lysate ...

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Abstract

The invention relates to an application of fluoxetine in an anti-mycobacterium tuberculosis drug. The fluoxetine hydrochloride is a first antidepressant known as a selective 5-hydroxytryptamine reuptake inhibitor (SSRIs), and the fluoxetine is a racemic mixture of R- and S- enantiomers, which have the same pharmacological activity; and the fluoxetine has anti-tuberculosis effect and has good development value. The use of fluoxetine in the anti-mycobacterium tuberculosis drug and the significant anti-tuberculosis effect of fluoxetine are disclosed for the first time.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to the application of fluoxetine in anti-tuberculosis mycobacterium drugs. Background technique [0002] Host anti-tuberculosis immunity and inflammatory response are closely related to the outcome of tuberculosis infection. Therefore, by interfering with host functions to increase host anti-TB immunity, the purpose of eradicating M. tuberculosis can be achieved. This strategy is called host-based gene-directed treatment of tuberculosis (HDT). HDT drugs achieve the purpose of treating tuberculosis by improving host anti-tuberculosis immunity, thus providing a new vision for improving the efficacy of tuberculosis treatment and overcoming the problem of drug-resistant tuberculosis treatment . [0003] Fluoxetine hydrochloride was the first antidepressant known as selective serotonin reuptake inhibitors (SSRIs). Fluoxetine is a racemic mixture of R- and S-enantiomers with ide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/137A61P31/06
CPCA61K31/137A61P31/06
Inventor 蔡毅陈心春欧阳琪
Owner SHENZHEN UNIV
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