miRNA analog modified by locked nucleic acid and thiophosphoric acid and application of miRNA analog in preparation of antitumor drugs
An anti-tumor drug, phosphorothioate technology, applied in the direction of anti-tumor drugs, DNA / RNA fragments, drug combinations, etc., can solve problems such as unsatisfactory results, achieve great potential and application value, promote apoptosis, and enhance anti-bone marrow tumor effect
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[0021] The present invention is further described below in conjunction with embodiment.
[0022] 1. Materials and methods
[0023] 1.1. Cell culture
[0024] Human myeloma cell lines ARP1 and OCI-My5 and human breast cancer cell line MCF7 (MCF7 cell line: purchased from ATCC; ARP1 cell line: University of Iowa, USA; OCI-MY5 cell line: University of Iowa, USA). ARP1 and OCI-My5 use RPMI-1640 complete medium (HyClone TM , Utah, USA), MCF7 was cultured in DMEM (high glucose) complete medium (HyClone TM , Utah, USA) cultured. The complete medium contained 10% fetal bovine serum ( LifeTechnologies) and 1% double antibody ( Life Technologies). All cell lines were cultured at 37°C with 5% carbon dioxide.
[0025] 1.2. Modification of oligodeoxynucleotide and locked nucleic acid
[0026] The MiR-15a gene is located on human chromosome 13. The ODN of miR-15a was named OMM-15a, and it was improved by LNA modification and named LNA-15a. OMM-15a and LNA-15a are both synthesize...
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