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Method for extracting and isolating rhododendrin from leaves of Rhododendron przewalskii

A technology of rhododendron and rhododendron, which is applied in chemical instruments and methods, organic chemistry, sugar derivatives, etc., can solve the problems of complex separation methods, large amount of organic solvent, low content, etc., and achieves abundant resources, less resource consumption, and purity. high effect

Active Publication Date: 2019-10-25
NORTHWEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few literature reports on the extraction and isolation of rhododendron aureum. The only report is that rhododendron aureum was isolated from the leaves of Rhododendron aureum, with a content of 0.48%. The separation method is complicated. The amount of organic solvent is large, and the content is not high
So far, there is no report on the extraction and separation of rhododendron from Longshu Rhododendron

Method used

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  • Method for extracting and isolating rhododendrin from leaves of Rhododendron przewalskii
  • Method for extracting and isolating rhododendrin from leaves of Rhododendron przewalskii
  • Method for extracting and isolating rhododendrin from leaves of Rhododendron przewalskii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Crush the Rhododendron leaves of Longshu and pass through a 40-mesh sieve. Weigh 100g of Longshu Rhododendron leaf powder, add 500ml of petroleum ether, and reflux at 70°C for 2 hours; add 1000mL of absolute ethanol after the dregs are dried, heat reflux extraction at 80°C for 3 times, each time for 45min, and combine the extracts;

[0028] (2) Concentrate the extract until it has no alcohol smell, add 300ml of water to dissolve it, then add it to a 200g polyamide column, and then elute with 2000ml of distilled water;

[0029] (3) Collect the water eluate and concentrate it into a thick paste with a density of 1.05, then add 150ml of absolute ethanol and heat to dissolve; filter while it is hot, discard the insoluble matter, and let the filtrate cool to crystallize to obtain a light yellow powder;

[0030] (4) Add 45ml of absolute ethanol to the light yellow powder, heat to dissolve, filter while it is hot, discard the insoluble matter, let the filtrate cool and rec...

Embodiment 2

[0032] (1) Crush the Longshu Rhododendron leaves and pass through a 50-mesh sieve. Weigh 100g of Longshu Rhododendron leaf powder, add 700ml of petroleum ether and reflux at 80°C for 1 hour; add 800mL of absolute ethanol after the dregs are dried, heat reflux extraction at 75°C for 3 times, each time for 45min; combine the extracts

[0033] (2) Concentrate the extract until it has no alcohol smell, add 250ml of water to dissolve it, then add it to a 100g polyamide column, and then elute with 1500ml of distilled water;

[0034] (3) Collect the water eluate and concentrate it into a thick paste with a density of 1.08, then add 200ml of absolute ethanol and heat to dissolve; filter while it is hot, discard the insoluble matter, and let the filtrate cool to crystallize to obtain a light yellow powder;

[0035] (4) Add 50ml of absolute ethanol to the light yellow powder, heat to dissolve, filter while it is hot, discard the insoluble matter, let the filtrate cool and recrystallize,...

Embodiment 3

[0037] (1) Crush the Rhododendron leaves of Longshu and pass through a 60-mesh sieve. Weigh 100g of Longshu Rhododendron leaf powder, add 800ml of petroleum ether, and reflux at 65°C for 1.5h; add 1200mL of absolute ethanol after the dregs are dried, heat reflux extraction at 80°C for 3 times, each time for 45min; combine the extracts.

[0038] (2) After the extract is concentrated until it has no alcohol smell, add 200ml of water to dissolve it, then add it to a 300g polyamide column, and then elute with 2000ml of distilled water;

[0039] (3) Collect the water eluate, concentrate it into a thick paste with a density of 1.10, then add 200ml of absolute ethanol and heat to dissolve; filter while it is hot, discard the insoluble matter, and let the filtrate cool to crystallize to obtain a light yellow powder;

[0040] (4) Add 80ml of absolute ethanol to the light yellow powder, heat to dissolve, filter while it is hot, discard the insoluble matter, let the filtrate cool and rec...

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Abstract

The invention provides a method for extracting and isolating rhododendrin from leaves of Rhododendron przewalskii. The method comprises adding leaf powder of Rhododendron przewalskii into a petroleumether to carry out refluxing; airing the medicinal residue, adding the dried medicinal residue into anhydrous ethyl alcohol, carrying out reflux extraction three times, and combining the extracts; concentrating the extract combination until no alcohol odor occurs, adding water to dissolve the concentrate, adding the dissolution into a polyamide column, and eluting with distilled water; collectingwater eluent, concentrating the water eluent into a thick paste having a density of 1.05-1.15, adding anhydrous ethyl alcohol, and heating for dissolving; filtering when the dissolution is hot, removing insolubles, and allowing the filtrate to cool and crystallize to obtain light yellow powder; adding anhydrous ethyl alcohol into the light yellow powder, heating for dissolving, filtering the dissolution while it is hot, removing insolubles, and allowing the filtrate to cool and recrystallize to obtain colorless needle-like crystal. It is determined by identifying the extracted rhododendrin that the extract is rhododendrin. The yield of rhododendrin is 3.5-4.5%, and the purity is 95-98%. The method is simple, free of toxicity and environmentally friendly and is suitable for industrial production.

Description

technical field [0001] The invention relates to a method for extracting and isolating white rhododendron from rhododendron leaves in Longshu, and belongs to the technical field of extraction and separation of plant effective active components. Background technique [0002] Rhododendrin, English name: Rhododendrin; CAS number: 497-78-9; Molecular formula: C 16 h 24 o 7 ;Molecular weight: 328.35756. Its structural formula: [0003] [0004] It has been reported in the literature that Rhododendron albicans has analgesic and anti-inflammatory effects. At present, there are few literature reports on the extraction and separation of Rhododendron albicans, and the only report is that Rhododendron in cowhide ( Rhododendron aureum ) leaves white rhododendron, the content is 0.48%, the separation method is complicated, the amount of organic solvent is large, and the content is not high. [0005] Longshu Rhododendron ( Rhododendron przewalskii Maxim.) is a Rhododendronaceae ...

Claims

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Application Information

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IPC IPC(8): C07H15/18C07H1/08
CPCC07H15/18C07H1/08
Inventor 梁俊玉逯佩玉邵亚洲徐婕杨盈盈王俊龙孔维宝
Owner NORTHWEST NORMAL UNIVERSITY
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