A kind of antiallergic peptide and preparation method thereof

Abstract

The invention discloses an antiallergic peptide and a preparation method thereof, comprising the following steps: S1. Preparation of Atlantic salmon offal enzymatic hydrolyzate: mix 12% substrate in distilled water (w / v) and pepsin, and then use 1M Adjust the pH to 2.0 with HCl, incubate at 37°C for 8 hours to release anti-allergic peptides, heat at 100°C for 15 minutes to inactivate pepsin, and after cooling, use a 0.45 μm filter membrane for suction filtration to remove unhydrolyzed protein. The invention adopts enzymolysis technology to enzymatically treat Atlantic salmon by-products, and adopts different in vitro evaluation methods and continuous chromatographic techniques to prepare, purify and identify active peptides with anti-allergic effects. The organic combination of industrial fish by-products and active peptides has been realized, and a new high-value and full utilization path has been opened up for the processing and treatment of industrial fish waste while planning to obtain anti-allergic active peptides, so that it can play a role in the field of anti-allergic.

Description

technical field [0001] The invention relates to the technical field related to biological research, in particular to an antiallergic peptide and a preparation method thereof. Background technique [0002] Allergic diseases include a wide variety of diseases, such as food allergies, atopic dermatitis, asthma and allergic rhinoconjunctivitis, etc. Although allergic diseases are not a serious disease like many complex diseases, their harm It should not be underestimated that for society as a whole, patients with allergies are inefficient at work, causing huge economic losses to countries, and need to be treated with corresponding antiallergic drugs, which are currently on the market in order to minimize their defects For the purpose of continuous updating and ensuring its safety, how to obtain a more natural and safe anti-allergic matrix for the development of anti-allergic drugs is the top priority. At present, there are related researches at home and abroad. The reported anti...

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Problems solved by technology

[0004] The object of the present invention is to provide an anti-allergic peptide and its preparation method to solve the problem of how to extract the immunomodulatory peptide with anti-allergic activity from the by-products produced during the processing of Atlantic frogfish mentioned in the above-mentioned background technology question

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