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Diluent and preservation method of sheep semen

A preservation method and diluent technology, which are applied in the field of diluent and preservation of sheep semen, can solve the problems of restricting the promotion of diluent of sheep semen, high requirements for refrigerated storage conditions, and few types of diluent, so as to protect the survival of sperm and reduce the Cell freezing point, the effect of improving sperm motility

Active Publication Date: 2021-07-27
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few types of diluents for sheep semen, and the requirements for cold storage conditions are high, and the cost is high, which limits the wide promotion of diluents for sheep semen

Method used

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  • Diluent and preservation method of sheep semen
  • Diluent and preservation method of sheep semen
  • Diluent and preservation method of sheep semen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A dilution of goat semen, the dilution includes the following components per 100mL: Y-27632 2HCl, Vitamin E 4mL, fresh egg yolk 20mL, glycerin 4mL, fructose 1.26g, citric acid monohydrate 1.72g, trishydroxymethylaminomethane 3.53g, vitamin C 0.5g, bovine serum albumin 0.5g, penicillin sodium 15000IU, streptomycin sulfate 15000IU, the balance is double distilled water. Prepare 5 groups of dilutions, group 1: blank control, Y-276322HCl 0mg; group 2: Y-276322HCl 0.1601mg / 100mL (corresponding molar concentration is 5mM); group 3: Y-276322HCl 0.6405mg / 100mL (corresponding molar concentration is 20mM); Group 4: Y-276322HCl 1.6013mg / 100mL (corresponding to a molar concentration of 50mM); Group 5: Y-276322HCl 3.2026mg / 100mL (corresponding to a molar concentration of 100mM).

Embodiment 2

[0031] The preservation method of embodiment 2 sheep semen

[0032] 1. Semen collection

[0033] The semen collection site is fixed on the breeding sheep farm affiliated to the experimental station. Before the semen collection, the rams are put into the shed, and the safety frame for the ewes is placed in the shed. Before the semen collection, the sanitation in the shed is cleaned. Find a healthy, disease-free, strong, and docile ewe in estrus in the ewe house, and fix it on the safety frame to ensure that the ewe is within the sight of the male animal, so as to arouse the sexual desire of the ram. Clean the false vagina, semen collection cup and glass rod, install the prepared semen collection cup at one end of the false vagina, then pour 50-60°C hot water into the interlayer of the false vagina, and install the air nozzle with piston; Use a glass rod to take a small amount of Vaseline, and evenly apply a layer of foam on the inner tube. After blowing and pressurizing, it is...

Embodiment 3

[0068] A dilution of sheep semen, the dilution includes the following components per 100 mL: Y-27632 2HCl 0.6405 mg, vitamin E 3.5 mL, fresh egg yolk 19 mL, glycerin 3.5 mL, fructose 1.25 g, citric acid monohydrate 1.7 g, Tris 3.5g, vitamin C 0.4g, bovine serum albumin 0.4g, penicillin sodium 10000IU, streptomycin sulfate 10000IU, the balance is double distilled water.

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PUM

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Abstract

The invention relates to a diluent of goat semen and a preservation method. The diluent contains the following components per 100mL: Y‑27632 2HCl 0.16~3.20mg, vitamin E 3.5~4.5mL, fresh egg yolk 19~21mL, fructose 1.2~1.3g, citric acid monohydrate 1.7~1.8g, three Hydroxymethylaminomethane 3.5-3.6g, vitamin C 0.4-0.6g, bovine serum albumin 0.4-0.6g, penicillin sodium 10000-15000IU, streptomycin sulfate 10000-15000IU, glycerin 3.5-4.5mL, the balance is double distilled water. The invention effectively prolongs the storage time of sheep semen under low temperature conditions, and improves the quality of preserved sperm.

Description

technical field [0001] The invention relates to a diluent of semen, in particular to a diluent of goat semen and a preservation method. Background technique [0002] my country has a long history of sheep breeding, and the germplasm resources of sheep and goats are abundant, among which the number of breeding accounts for the first in the world. The sheep raising industry is of great significance to the modernization of the country. The sheep raising industry has great development prospects in the vast grasslands and rural areas. The rapid development of the sheep raising industry can not only contribute to the country's reform and opening up, but also improve people's living standards. With the continuous advancement of science and technology, my country's sheep breeding industry has also made great progress, but there are still many problems. Compared with developed countries, there is still a big gap, especially in terms of large-scale breeding and intensive breeding. In...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0215A01N1/0221A01N1/0226
Inventor 曹鸿国李彩霞唐云书孙寒雪高晋凤涂丹凤卢振涛张苍龙孟祝张子军任春环陈家宏刘亚周建军陈宏权
Owner ANHUI AGRICULTURAL UNIVERSITY
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