Co-dominant marker primer set and identification method for identifying tobacco resistance spotted wilt disease locus RTSW allele types, and applications thereof

A technology of co-dominant markers and alleles, applied in the field of molecular biology, can solve the problems of inability to distinguish homozygous/heterozygous genotypes of resistance sites, false positives of SCAR markers, and limit the large-scale application of markers. Significant difference in belt type, high detection efficiency, and reduced detection cost

Active Publication Date: 2019-11-26
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, only H. Moon and J.S. Nicholson (2007) have developed the corresponding molecular markers in the world, but the genetic distance between the AFLP marker and the resistance gene is far away, and the linkage is not tight. The developed SCAR markers exist in practical applications. prone to false positives
Moreover, neither marker can distinguish homozygous / heterozygous genotypes at the resistance locus, limiting the large-scale application of these markers

Method used

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  • Co-dominant marker primer set and identification method for identifying tobacco resistance spotted wilt disease locus RTSW allele types, and applications thereof
  • Co-dominant marker primer set and identification method for identifying tobacco resistance spotted wilt disease locus RTSW allele types, and applications thereof
  • Co-dominant marker primer set and identification method for identifying tobacco resistance spotted wilt disease locus RTSW allele types, and applications thereof

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Embodiment Construction

[0036] The present invention will be further described in detail below in conjunction with the examples.

[0037] A co-dominant molecular marker primer set for identifying the RTSW allele type of tobacco spotted wilt locus, the molecular marker is the sequence shown in Seq ID No.5 or Seq ID No.6, and the molecular marker can be composed of primer 1 , Primer 2, Primer 3 and Primer 4 were amplified. The primer set is composed of four single-stranded DNAs of primer 1, primer 2, primer 3 and primer 4.

[0038] The primer 1 sequence is Seq ID No.1:

[0039] RTSW_Marker2_F 5'-GTTATAAATTGTGTGGAGGGCATG-3';

[0040] The primer 2 sequence is Seq ID No.2:

[0041] RTSW_Marker2_R 5'-TCAAATTCCCGACCAGCAGTA-3';

[0042] The primer 3 sequence is Seq ID No.3:

[0043] rtsw_Marker1_F 5'-CCGGAATAAGGGTAAGGTCTG-3';

[0044] The primer 4 sequence is Seq ID No.4:

[0045] rtsw_Marker1_R 5'-CAACAACAACAATCGGGTGA-3'.

[0046] Seq ID No.5 sequence:

[0047] GTTATAAATTGTGTGGAGGGCATGCCCTTCCAATTTT...

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Abstract

The present invention discloses a co-dominant marker primer set and an identification method for identifying tobacco resistance spotted wilt disease locus RTSW allele types, and applications thereof.The primer set is composed of four single-stranded DNAs of a primer 1, a primer 2, a primer 3 and a primer 4. The co-dominant marker primers can specifically mark and identify tobacco genomic DNA, detect the RTSW allele types in the tobacco material genome DNA, can also identify whether contains tobacco resistance spotted wilt disease locus RTSW, can also identify the tobacco resistance spotted wilt disease locus RTSW allele types, are reliable and easy to use, have advantages of tight linkage with the target gene RTSW, high accuracy, low cost, high detection efficiency, etc. compared with previous spotted wilt disease resistance markers, can greatly improve screening efficiency of tobacco resistance spotted wilt disease gene RTSW varieties, greatly shorten breeding cycles of disease resistance tobacco varieties, and improve breeding efficiency.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to molecular marker primers capable of identifying tobacco spot wilt resistance gene locus RTSW and its application in the positioning, cloning or breeding of tobacco spot wilt resistance gene loci . Background technique [0002] Tobacco spotted wilt is a disease of tobacco caused by the infection of Orthotospovirus. This genus is a plant virus with the widest range of hosts and the most serious occurrence. Its representative American species, Tomato Spotted Wilt Virus (TSWV), has posed a greater threat to tobacco leaf production in Yunnan tobacco-growing areas in my country. . According to the test results of samples collected from various places (states, cities) and counties in Yunnan in recent years, TSWV is distributed on tobacco in Yunnan province and tends to expand and intensify. What is even more worrying is that none of the main flue-cured tobacco va...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 黄昌军刘勇童治军
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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