Co-dominant marker primer capable of distinguishing homozygous genotype and heterozygous genotype of tobacco locus RTSW with resistance to spotted wilt, distinguishing method and application of co-dominant marker primer capable of distinguishing homozygous genotype and heterozygous genotype of tobacco locus RTSW with resistance to spotted wilt

Abstract

The invention discloses a co-dominant marker primer capable of distinguishing homozygous genotype and heterozygous genotype of a tobacco locus RTSW with resistance to spotted wilt, a distinguishing method and application of the co-dominant marker primer capable of distinguishing the homozygous genotype and the heterozygous genotype of the tobacco locus RTSW with resistance to spotted wilt. The primer is composed of two strands of single-stranded DNA of a primer 1 and a primer 2. The co-dominant marker primer can specifically mark and identify tobacco genome DNA to detect the type of an alleleof the RTSW in the tobacco material genome DNA, and not only can whether or not the locus RTSW with resistance to spotted wilt is contained be identified, but also the homozygous genotype and the heterozygous genotype of the tobacco locus RTSW with resistance to spotted wilt can be distinguished, so that the co-dominant marker primer is reliable and convenient to use, compared with prior markers with resistance to spotted wilt, the co-dominant marker primer has the advantages that the co-dominant marker primer is in close linkage with the target gene RTSW, the accuracy is high, the cost is low, the detection efficiency is high, and the like, the efficiency of screening a variety with the gene RTSW with resistance to spotted wilt of tobacco can be greatly improved, a breeding cycle of the disease-resistant tobacco variety is greatly shortened, and the breeding efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, in particular to co-dominant marker primers capable of distinguishing RTSW homozygous heterozygous genotypes of RTSW resistance sites in tobacco and its location, cloning or selection of RTSW resistance genes in tobacco Application in spotted wilt varieties. Background technique [0002] Tobacco spotted wilt is a disease of tobacco caused by the infection of Orthotospovirus. This genus is a plant virus with the widest range of hosts and the most serious occurrence. Its representative American species, Tomato Spotted Wilt Virus (TSWV), has posed a greater threat to tobacco leaf production in Yunnan tobacco-growing areas in my country. . According to the test results of samples collected from various places (states, cities) and counties in Yunnan in recent years, TSWV is distributed on tobacco in Yunnan province and tends to expand and intensify. What is even more worrying is that none ...

AI Technical Summary

Problems solved by technology

So far, only H. Moon and J.S. Nicholson (2007) have developed the corresponding molecular markers in the world, but the genetic distance between the AFLP marker and the resistance gene is far away, and the linkage is not tight. The developed SCAR markers exist in practical applications. prone to false positives

Moreover, neither marker can distinguish homozygous / heterozygous genotypes at the resistance locus, limiting the large-scale application of these markers

Images