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Application of Fibroblast Growth Factor Receptor 1 in the Preparation of Drugs for Prevention and Treatment of Enterovirus 71 Infection

A growth factor receptor, fibroblast technology, applied in the field of biomedicine

Active Publication Date: 2021-10-15
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] However, there are no reports on the role of IL4R and FGFR1 molecules in enteroviruses, especially EV71-infected host cells. In-depth research on these two molecules will not only improve the understanding of EV71 infection and pathogenic mechanisms, but also provide Prevention and treatment of EV71 infection provides new ideas and targets

Method used

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  • Application of Fibroblast Growth Factor Receptor 1 in the Preparation of Drugs for Prevention and Treatment of Enterovirus 71 Infection
  • Application of Fibroblast Growth Factor Receptor 1 in the Preparation of Drugs for Prevention and Treatment of Enterovirus 71 Infection
  • Application of Fibroblast Growth Factor Receptor 1 in the Preparation of Drugs for Prevention and Treatment of Enterovirus 71 Infection

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Embodiment 1

[0062] 1 Design, synthesize specific siRNA sequences of each transport-related membrane protein.

[0063] 1.1 For each target gene, the NCBI GeneBank is retrieved to obtain a full sequence and mRNA sequence, and the existing network resources and common software are biological analysis, and the coding region is selected as a target sequence for siRNA design. Refer to the siRNA design principle, and compared to the human genome sequence via the GeneBank database, it is ensured that there is no creature; the 5 'end of the AITISENSE chain is eliminated with the potential siRNA with other genes paired; exclude any section of continuous 14 Potential siRNA paired with other genes. And using design software for pre-evaluation, three optimal dynamics parameter targets enter the subsequent experimental process, each genetically synthesize three interference sequences, see Table 1.

[0064] 1.2 Synthesis and purification of single-stranded siRNA was completed by Invitrogen.

[0065] Table 1...

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Abstract

The invention relates to the technical field of biomedicine, in particular to a new target for resisting enterovirus 71 infection and its application. In the present invention, human colon cancer cells (Caco-2) are used as target cells, and RNA interference technology is used to down-regulate the expression of target cell membrane receptors to find host factors that can effectively inhibit EV71 from infecting human colon cancer cells. The purpose of blocking EV71 infection. The present invention finds through experiments that fibroblast growth factor receptor 1 (FGFR1) plays an important role in the infection of Caco-2 cells by EV71, and downregulating the expression of FGFR1 can obviously inhibit the infection of EV71. The present invention provides the application of FGFR1 in the preparation of drugs for preventing or treating enterovirus 71 infection, and provides clinical prevention and treatment for diseases such as hand, foot and mouth disease, meningitis, brainstem encephalitis or polio caused by EV71 infection. new targets and treatment options.

Description

Technical field [0001] The present invention relates to the field of biomedical technology, in particular, a new target and application of an intestinal virus 71 infection. Background technique [0002] The intestinal virus 71 (ENTEROVIRUS 71, EV71) belongs to the small core-foot-mouth dissease, HFMD, which leads to the main pathogen of Hand-Foot-mouth Disease (HFMD). At present, the hand and foot and mouth disease have entered and popular in multiple regions of the world, especially in the Asia-Pacific region. The main cases of hand and foot and mouth disease are infants under the age of 5. The clinical manifestations are symptoms such as herpes, hands, foot, buttocks, and oral mucosa. There are symptoms such as herpes; minority children can develop into severe patients, the central nervous system (Central Nervous System) , CNS) lesions, including sterile meningitis, brain, cerebrosphenesis, and neuronal pulmonary edema, serious threatening of infant life health [Solomon T, et a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K45/00A61P31/14
CPCA61K45/00A61P31/14
Inventor 朱勇喆陈洋张展榕刘延刚赵平戚中田
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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