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A nerve substrate catheter for nerve repair and its preparation method

A nerve repair and nerve technology, applied in the field of nerve repair nerve matrix catheter and its preparation, can solve the problems of mismatched nerve size, non-degradable nerve repair catheter material, lack of autologous nerve transplantation sources, etc., to promote and induce nerve regeneration. , reducing immunogenicity, no immunogenicity excellent effect

Active Publication Date: 2021-08-24
CHONGQING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a nerve matrix conduit for long-distance nerve repair, which solves the problem of lack of sources for autologous nerve transplantation, size mismatch between donor and damaged nerve, and non-degradable nerve repair conduit Material needs secondary surgery and other issues

Method used

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  • A nerve substrate catheter for nerve repair and its preparation method
  • A nerve substrate catheter for nerve repair and its preparation method
  • A nerve substrate catheter for nerve repair and its preparation method

Examples

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Effect test

Embodiment 1

[0034] One, a kind of natural nerve substrate catheter, adopts following method to make:

[0035] 1) Take the sciatic nerve from a healthy rat, and then bathe it in a Triton X-100 solution with a concentration of 3% (m / v) (which needs to be sterilized with a 0.22 μm filter membrane) at 50 r / v min speed for 12 hours, and change the water every 4 hours; after 12 hours, wash the nerve with distilled water for 2-3 times, and then place it in 3% (m / v) Triton X-100 solution for 12 hours; The nerve tissue was degreased with methanol for 24 h (the methanol solution was replaced twice during the period), and then the nerve tissue was treated with 2 μL / mL (v / v) DNase diluent at 37 °C for 2 h. Finally, the decellularized nerve tissue was obtained by washing and drying with absolute ethanol and water in sequence. After decellularization, the nerve tissue became loose, white strips, and slightly reduced in size ( figure 1 ).

[0036] 2) After the decellularized nerve tissue was crushed, ...

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Abstract

The invention discloses a nerve matrix conduit for nerve repair and a preparation method thereof. The conduit is formed by compounding and solidifying decellularized nerve matrix, chitosan and sodium glycerophosphate. The natural gel matrix catheter prepared by the invention has a stable structure, is not suitable for deformation, and has the resilience of gel, and the inner diameter hole of the catheter is smooth and runs through, and the catheter has good temperature sensitivity on the basis of good biocompatibility And plasticity, when the temperature rises to the physiological body temperature of 37 ℃, gelation will occur immediately, and the gelled catheter can be stored at room temperature for a long time, which can meet the needs of different tissues in the human body; it has controllable degradation and is suitable for different Nerve defect repair; low immunity and inducing nerve regeneration, which is conducive to cell adhesion and accelerates the process of nerve regeneration. The preparation process of the invention is simple, easy to operate and control, and has broad application prospects.

Description

technical field [0001] The invention relates to the technical field of tissue engineering materials, in particular to a nerve substrate conduit for nerve repair and a preparation method thereof. Background technique [0002] The repair and reconstruction of peripheral nerve defect is a major problem in the field of peripheral nerve injury. For short-segment nerve injuries, the severed nerve can be directly anastomosed end-to-end, so that the proximal regenerated nerve fibers can grow into the distal end. However, for long-segment nerve defects, it is currently impossible to repair them clinically. With the development of tissue engineering technology, the use of nerve guides and seed cells to construct tissue-engineered peripheral nerves in vitro to replace autologous nerve transplantation has become a development direction. [0003] The nerve conduit is a carrier for repairing nerve defects. It has the advantages of adjustable diameter and length, tension-free suture at t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/20A61L27/56A61L27/58
CPCA61L27/20A61L27/3604A61L27/3675A61L27/3687A61L27/56A61L27/58A61L2430/32C08L5/08
Inventor 陈国宝黄湘付强熊建夏斌王富平陈忠敏
Owner CHONGQING UNIV OF TECH
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