Optimized model for rapid identification of transgenic soybeans based on morphological analysis

A technology for genetically modified soybeans and morphological analysis, applied in the field of near-infrared spectroscopy detection, can solve the problems of inapplicability to actual conditions and time-consuming
CN110632017AInactive Publication Date: 2019-12-31NAT INST FOR NUTRITION & HEALTH CHINESE CENT FOR DISEASE CONTROL & PREVENTION
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NAT INST FOR NUTRITION & HEALTH CHINESE CENT FOR DISEASE CONTROL & PREVENTION
Publication Date
2019-12-31
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides an optimized model for rapid identification of transgenic soybeans based on morphological analysis. According to the invention, firstly, an establishment method of the optimizedmodel for rapid identification of the transgenic soybean based on morphological analysis is provided; for whole soybeans, spectral information under a characteristic wave band of 9403-5438cm<-1> is selected, the spectrum is preprocessed by adopting a second derivative, and a PLS-DA model is established by adopting a partial least squares-discrimination method; and for powdery soybeans, spectral information under a characteristic waveband of 7505-4597cm<-1> is selected, the spectrum is preprocessed by adopting vector normalization and a first derivative, and a PLS-DA model is established by adopting a partial least squares-discrimination method. According to the invention, the transgenic soybeans are identified by combining the near-infrared spectrum with a discriminant analysis method, and the identification accuracy of the discrimination model can be improved by selecting the sample form, the wavelength range and the spectrum pretreatment method, so that the optimal model is selectedto be applied to actual production.
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Description

technical field

[0001] The invention belongs to the technical field of near-infrared spectrum detection, and in particular relates to a method for quickly identifying transgenic soybeans by using near-infrared spectrum. Background technique

[0002] Soy plays an important role in the traditional diets of many countries, mainly because it is low in fat and an excellent source of protein, dietary fiber and various micronutrients and phytochemicals. It is hoped that genetic technology can be used to increase the yield of soybeans or to change the characteristics of soybeans, such as improving the nutritional content of seeds (including protein, oil and some active ingredients), improving the resistance of seeds to diseases and insect pests, or planting in new geographical areas Develop new varieties, etc. Based on the purpose of implementing quality control on genetically modified soybeans, it is necessary to develop some reliable and effective detection methods for geneticall...

Claims

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