Resurrection plant selaginella pulvinata maxim tissue culture propagation method

A technology for resuscitation of Selaginella mats, applied in the field of plant reproduction, can solve the problems of insufficiency, easy browning of Selaginella mats, difficulty in tissue culture propagation, etc., and achieves the effect of high reproduction efficiency

Active Publication Date: 2020-02-18
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, Selaginella cuspidatum has both ornamental and medicinal value, and wild resources have been extensively exploited, resulting in a sharp decline in the wild population of Selaginella cuspidatum, and wild resources can no longer meet the current demand
[0004] In the Selaginella genus, some species with creeping main stems, such as: Xiaocuiyun, have adventitious roots at the stem nodes, which can be propagated by cuttings, but the main stems of Selaginella cushions are not creeping, so it is extremely difficult to propagate by cuttings
In addition, it was found in the previous research that browning of Selaginella cuspidatum is very easy to occur during the tissue culture process, and the tissue culture propagation is difficult
At present, there is no report on the related propagation technology of the revived plant Selaginella cuspidatum, nor the tissue culture propagation method of the revived plant Selaginella cuspidatum

Method used

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  • Resurrection plant selaginella pulvinata maxim tissue culture propagation method
  • Resurrection plant selaginella pulvinata maxim tissue culture propagation method
  • Resurrection plant selaginella pulvinata maxim tissue culture propagation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 The inventive method

[0028] (1) Acquisition of sterile explants

[0029] Select 0.8-1.2 cm young shoot tips of wild Selaginella cushion-like plants as explants, clean the surface of the explants with a soft brush dipped in 1% v / v detergent water, and then place them in a beaker. Cover with gauze and rinse with running tap water for 2 hours. Under aseptic conditions, the explants were sterilized with a disinfectant for 12 minutes, and then rinsed with sterile water for 5 times for 3 minutes each time to obtain sterile explants. The disinfectant is HgCl at a concentration of 0.1% w / v 2 solution. The young shoots are the 2nd to 3rd layers of shoots from the center growth point of the Selaginella cushion-like plant.

[0030] (2) Induction of primary buds

[0031] The sterile explants obtained in step (1) were inoculated on the primary bud induction medium, placed in a 22°C culture room for dark cultivation, the dark culture period was 42 days, and the pr...

Embodiment 2

[0039] Embodiment 2 and embodiment 3 are the method of the present invention

[0040] Embodiment 2 and embodiment 3 are different except that the measures listed in table 1 are different, and all the other measures are the same as embodiment 1, and will not be repeated.

[0041]Table 1 embodiment 2 and embodiment 3 are different from the measures of embodiment

[0042]

[0043] The reproductive effect of table 2 embodiment 1-3

[0044]

[0045] control

[0046] The control is the reproduction of wild plants of Selaginella cuspidatum in the wild habitat.

[0047] (1) Observation objects: 30 2- to 3-year-old wild plants of Selaginella cuspidatum with consistent growth were selected as the observation group in the same growth area in the wild.

[0048] (2) Field habitat: bare limestone surface with extremely thin soil layer; dry season and rainy season are distinct, and the annual average precipitation is about 600-800 mm, of which, the dry season precipitation accounts ...

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Abstract

The invention discloses a resurrection plant selaginella pulvinata maxim tissue culture propagation method which includes the steps of selaginella pulvinata maxim sterile explant acquisition, primarybud inducing, adventitious bud proliferation, tissue culture seedling strengthening and rooting culture. Resurrection plant selaginella pulvinata maxim immature branch tips successfully serve as explants, a selaginella pulvinata maxim tissue culture propagation system is built, the proliferation rate of adventitious buds reaches 8 times or more, the re-proliferation rate of single adventitious buds in the tissue culture seedling strengthening and rooting culture process reaches 11 times or more, one selaginella pulvinata maxim immature branch tip can be subjected to expanding propagation within 170-175 days to obtain 88 or more tissue culture seedlings, higher propagation efficiency is acquired, a good foundation is laid for large-scale expanding propagation of selaginella pulvinata maxim,and the method is of great significance for wild population conservation and resource development and utilization of the resurrection plant selaginella pulvinata maxim.

Description

technical field [0001] The invention belongs to the field of plant propagation, and in particular relates to a tissue culture propagation method for revived plant Selaginella cuspidatum. Background technique [0002] Selaginella pulvinata (Hook.&Grev) Maxim.], belonging to Selaginellaceae (Selaginellaceae) Selaginella genus, is cushion-shaped, the main stem is not creeping, and mainly grows on exposed limestone surfaces or stone crevices. The growing environment is relatively dry. Selaginella cuspidatum, commonly known as "Nine Dead Resurrection Grass", is a typical recovery plant with strong dehydration resistance. It can maintain long-term survival through low metabolism under extreme dehydration conditions. When water conditions are suitable, it can quickly return to normal. grow. Selaginella cuspidatum has become a special class of ornamental plant resources because of its magical recovery properties. At the same time, Selaginella cuspidatum contains flavonoids, bifla...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 余蓉培汪国鲜浦艳飞李帆卢珍红莫锡君
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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