Duplex test strip for detecting morphine and ketamine as well as preparation method and application method of duplex test strip

A technology of ketamine and test strips, applied in the field of double test strips and its preparation, can solve the problems of inability to realize rapid detection of large batches of samples, simultaneous detection of morphine and ketamine, complicated operation, etc., and achieve the elimination of non-specific fluorescence No interference, not limited by detection equipment, simple operation

Inactive Publication Date: 2020-03-27
郑州左安检测科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to overcome the shortcomings of the existing methods for detecting morphine and ketamine, which are complex in operation, time-consuming, unable to realize rapid detection of large batches of samples, and unable to detect morphine and ketamine at the sam...

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  • Duplex test strip for detecting morphine and ketamine as well as preparation method and application method of duplex test strip
  • Duplex test strip for detecting morphine and ketamine as well as preparation method and application method of duplex test strip
  • Duplex test strip for detecting morphine and ketamine as well as preparation method and application method of duplex test strip

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preparation example Construction

[0045] The preparation method of the double test strip for detecting morphine and ketamine mainly comprises the following steps:

[0046] 1) labeling morphine antibody and ketamine antibody with fluorescent microspheres, and spraying it on the conjugate release pad to prepare a conjugate release pad sprayed with fluorescent microsphere-labeled morphine antibody and ketamine antibody;

[0047] 2) Spray the morphine hapten-carrier protein conjugate, ketamine hapten-carrier protein conjugate and goat anti-mouse secondary antibody (or Biotin-BSA) on the reaction membrane respectively as detection lines T1, T2 and quality control lines C, to prepare detection line T1 coated with morphine hapten-carrier protein conjugate, detection line T2 coated with ketamine hapten-carrier protein conjugate and coated with goat anti-mouse secondary antibody (or Biotin-BSA) The reaction membrane of quality control line C;

[0048] 3) Assemble the conjugate release pad prepared in 1) and 2), the re...

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Abstract

The invention relates to a duplex test strip for detecting morphine and ketamine as well as a preparation method and an application method of the duplex test strip. The test strip sequentially comprises a sample absorption pad, a conjugate release pad, a reaction membrane, a water absorption pad and a bottom plate, a morphine antibody and a ketamine antibody marked by the time-resolved fluorescentmicrospheres are sprayed on the conjugate release pad, and the reaction membrane is coated with a detection line T1 of a morphine hapten-carrier protein conjugate, a detection line T2 of a ketamine hapten-carrier protein conjugate and a quality control line C of a goat anti-mouse secondary antibody (or Biotin-BSA). According to the invention, the antibodies are labeled by the time-resolved fluorescent microspheres, and the simultaneous and rapid immunoassay of morphine and ketamine is realized by adopting an immunochromatography technology. The invention also provides a method for simultaneously detecting morphine and ketamine in a sample by using the above test strip. The duplex test strip and the method have the advantages of being high in sensitivity and rapid in detection, being capable of achieving accurate quantification, being convenient to operate, being economical and practical, and being capable of achieving the rapid detection and on-site detection on a large batch of samples.

Description

technical field [0001] The invention relates to the detection of morphine and ketamine, in particular to a dual test strip for simultaneous quantitative detection of morphine and ketamine by using time-resolved fluorescent microsphere immunochromatography technology, and a preparation method and application method thereof. Background technique [0002] Morphine is the main component of opium, heroin, etc. It is extracted from opium and is a pure opioid receptor agonist. It has analgesic and hypnotic effects. Therefore, in medicine, morphine is a narcotic analgesic. Morphine has the function of inhibiting the central nervous system, and it is easy for eaters to develop tolerance and dependence, and it is easy to become addicted and abused. [0003] Ketamine, a dissociative anesthetic, is a derivative of phencyclidine (PCP), commonly known as "K powder", and is generally used as an anesthetic in clinical practice. Ketamine can excite blood vessels, overdose can cause death, a...

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Application Information

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IPC IPC(8): G01N33/577G01N33/558G01N33/543G01N21/64
CPCG01N21/6428G01N33/54313G01N33/558G01N33/577
Inventor 贾嘉胡思敏陈丽丽蔡秋念孟二娟吕小翠
Owner 郑州左安检测科技有限公司
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