Duplex test strip for detecting morphine and ketamine as well as preparation method and application method of duplex test strip

Abstract

The invention relates to a duplex test strip for detecting morphine and ketamine as well as a preparation method and an application method of the duplex test strip. The test strip sequentially comprises a sample absorption pad, a conjugate release pad, a reaction membrane, a water absorption pad and a bottom plate, a morphine antibody and a ketamine antibody marked by the time-resolved fluorescentmicrospheres are sprayed on the conjugate release pad, and the reaction membrane is coated with a detection line T1 of a morphine hapten-carrier protein conjugate, a detection line T2 of a ketamine hapten-carrier protein conjugate and a quality control line C of a goat anti-mouse secondary antibody (or Biotin-BSA). According to the invention, the antibodies are labeled by the time-resolved fluorescent microspheres, and the simultaneous and rapid immunoassay of morphine and ketamine is realized by adopting an immunochromatography technology. The invention also provides a method for simultaneously detecting morphine and ketamine in a sample by using the above test strip. The duplex test strip and the method have the advantages of being high in sensitivity and rapid in detection, being capable of achieving accurate quantification, being convenient to operate, being economical and practical, and being capable of achieving the rapid detection and on-site detection on a large batch of samples.

Description

technical field [0001] The invention relates to the detection of morphine and ketamine, in particular to a dual test strip for simultaneous quantitative detection of morphine and ketamine by using time-resolved fluorescent microsphere immunochromatography technology, and a preparation method and application method thereof. Background technique [0002] Morphine is the main component of opium, heroin, etc. It is extracted from opium and is a pure opioid receptor agonist. It has analgesic and hypnotic effects. Therefore, in medicine, morphine is a narcotic analgesic. Morphine has the function of inhibiting the central nervous system, and it is easy for eaters to develop tolerance and dependence, and it is easy to become addicted and abused. [0003] Ketamine, a dissociative anesthetic, is a derivative of phencyclidine (PCP), commonly known as "K powder", and is generally used as an anesthetic in clinical practice. Ketamine can excite blood vessels, overdose can cause death, a...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the shortcomings of the existing methods for detecting morphine and ketamine, which are complex in operation, time-consuming, unable to realize rapid detection of large batches of samples, and unable to detect morphine and ketamine at the same time, and provide an operation A simple, high-sensitivity, fast-detecting, and low-cost double-linked test strip for drug detection and its preparation method and application method, so as to realize simultaneous rapid detection and on-site monitoring of morphine and ketamine in a large number of samples

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