2results about How to "Accurate quantitative content" patented technology

This invention discloses a method for detecting L-alanine content in fermentation broth based on online derivatization using liquid chromatography: the fermentation broth to be tested is subjected to high-performance liquid chromatography (HPLC), with online automatic derivatization, detection by a UV detector, and quantification using the external standard method to obtain the L-alanine content; the chromatographic column is a C18 column, and the mobile phase is a mixture of acetonitrile and 0.01–0.06 mol / L phosphate buffer solution at pH 5–8; the detection wavelength is 260–350 nm; the derivatizing agent used in the online automatic derivatization is a solution of phthalaldehyde and β-mercaptoethanol dissolved in ethanol, or a solution of phthalaldehyde and 3-mercaptopropionic acid dissolved in ethanol. The method of this invention has good precision, with relative standard deviations (RSDs) all less than 2.0%. This invention uses a more versatile UV detector, which can eliminate fluorescence background interference, has high sensitivity, good repeatability, and high accuracy, and significantly reduces the amount of toxic reagents used, making it suitable for real-time monitoring and quality control in industrial fermentation processes.

This invention discloses a qualitative and quantitative analysis method for heparin-like components in the biopharmaceutical field. The method includes using a liquid chromatograph, an SAX strong anion exchange column, sodium dihydrogen phosphate solution as mobile phase A, and mobile phase B consisting of mobile phase A containing sodium perchlorate, with gradient elution for monitoring and analysis. This invention improves the separation of heparin-like components by screening chromatographic packing materials, optimizing the chromatographic method, and optimizing the detection wavelength. It allows for accurate qualitative analysis of up to five heparin components using a simple liquid chromatography method. Furthermore, this invention incorporates the simulated separation function of the Glycomapping glycosylation software to reconstruct the elution peaks of components that do not reach baseline separation, and then achieves quantitative analysis of each separated component based on the external standard method. This qualitative and quantitative analysis method provides a feasible solution for component identification and content determination in heparin-like samples, and offers an important means for heparin quality control.