33results about How to "Accurate quantitative content" patented technology

The invention discloses a test paper strip for detecting tetrahydrocannabinolate as well as a preparation method and an application method thereof. The test paper strip comprises a sample absorption pad, a conjugate release pad, a reaction membrane, a water absorbing pad and a substrate in sequence, wherein the conjugate release pad is coated with an antibody marked by time resolved fluorescence microspheres; the reaction membrane is coated with a detection line of hapten-carrier protein conjugate and a quality control line of a goat-anti-mouse second antibody. Due to adoption of the time resolved fluorescence marked antibody and an immunochromatography technique, rapid immunoassay of tetrahydrocannabinolate can be achieved. The invention further provides a method for detecting tetrahydrocannabinolate in a sample by using the test paper strip. The test paper strip has the advantages of being high in sensitivity, accurate in quantification, rapid in detection, convenient to operate, economical, and practical, and rapid detection and on-site detection on large scales of tetrahydrocannabinolate samples can be achieved.

The invention relates to a triple test strip for detecting methamphetamine, morphine and ketamine as well as a preparation method and an application method of the triple test strip. The test strip sequentially comprises a sample absorption pad, a conjugate release pad, a reaction membrane, a water absorption pad and a bottom plate, a methamphetamine antibody, a morphine antibody and a ketamine antibody marked by the time-resolved fluorescent microspheres are sprayed on the conjugate release pad, and the reaction membrane is coated with a detection line T1 of a methylamphetamine antigen, a detection line T2 of a morphine antigen, a detection line T3 of a ketamine antigen and a quality control line C of a goat anti-mouse secondary antibody or Biotin-BSA. According to the immunochromatographytechnology, the simultaneous and rapid immunoassay of three drugs including methamphetamine, morphine and ketamine is realized. The invention also provides a method for simultaneously detecting the methamphetamine, morphine and ketamine in a sample by using the above test strip. The triple test strip and the method have the advantages of being high in sensitivity and rapid in detection, being capable of achieving accurate quantification, being convenient to operate, and being capable of achieving the rapid detection and on-site detection on a large batch of samples.

The invention discloses a test strip for detecting ketamine and a preparation method and application method thereof. The test strip orderly comprises a sample absorption pad, a conjugate release pad,a reaction film, a water absorbent pad and a bottom plate. The conjugate release pad is spray-coated with a time-resolved fluorescent microsphere-labeled antibody. The reaction film is coated with a hapten-carrier protein conjugate detection line and a goat anti-mouse secondary antibody quality control line. Through the time-resolved fluorescent microsphere-labeled antibody and immunochromatography, ketamine can be used for fast immunoassay. The invention also provides a method for detecting ketamine in a sample through the test strip. The test strip has the advantages of high sensitivity, accurate quantification, rapid detection, convenient operation, economical and practical effects, and can realize rapid detection and on-site detection of a large amount of ketamine samples.

A multifunctional rapid food detector is provided with a motor used for driving upconversion fluorescence test strips to move, an adjustable-wavelength laser device module used for irradiating the upconversion fluorescence test strips, a bar code scanning module used for acquiring the ID identifier information of the upconversion fluorescence test strips, and an optical detection unit used for collecting visible light emitted by the upconversion fluorescence test strips. A unique ID identifier is set and attached to each upconversion fluorescence test strip, the signal input end of the motor and the signal input end of the adjustable-wavelength laser device module are connected with the control signal output end of a control unit, the signal output end of the bar code scanning module and the signal output end of the optical detection unit are connected with the signal input end of the control unit, the control unit is further connected with a cloud database through an internet module, the information output end of the control unit is connected with an SD memory card, and the information output end of the control unit is further connected with an LCD touch screen. The multifunctional rapid food detector has the advantages of being low in power consumption, high in precision and stability and suitable for detection occasions with various upconversion fluorescence test strips.

The invention discloses a method for determining the galactooligosaccharide content in milk powder, rice powder or milk and rice powder and application thereof.The method adopts an XAmide amide column and adopts ethanol and isopropyl alcohol according to the ratio of 1 to 4 in the pretreatment process of a sample to be detected to efficiently reduce lactose interference in the sample to be detected by being mixed with an organic solvent, the detection conditions are optimized, glucose, galactose and their epimers in an enzyme hydrolysis product are effectively separated, a linear regression equation is established, and the galactooligosaccharide content in the sample to be detected is accurately determined.The method is simple and easy to operate, the pretreatment time and costs are greatly reduced, a linear range is wide, and the method has the advantages of being low in interference, low in detection limit, high in accuracy and the like.

The invention provides a group of escherichia coli specific primers and probe sequences so as to establish a fluorescent quantitation PCR detection method which can be applied to detection of the content of escherichia coli in heart blood a dead rat and which is quick, sensitive and good in specificity. According to one side of the invention, specific primers and probes for detecting the escherichia coli are provided, and the specific primers and the probes use conservative fragment sequences as a target goal. A gene clone technique is adopted, escherichia coli LacZ gene specificity fragmentsare inserted into a carrier pMD18-T, so that recombinant plasmids containing LacZ gene specificity fragments are obtained and are used as standard products. A group of specific primers and probes aredesigned and synthesized according to escherichia coli LacZ gene specificity fragment sequences, the PCR reaction condition is optimized, a detection method by using a real-time fluorescent quantitation polymerase chain reaction as a platform is established, and the established method is evaluated.

The invention provides a specific primer and probe for real-time fluorescent PCR detection of enterococcus faecalis. The specific primer and probe for real-time fluorescent PCR detection of the enterococcus faecalis are characterized in that the nucleotide sequences of the specific primer and probe is one of (1) or (2), for (1), an upstream primer is 5'-TTCGCACCAGGAGATAAA-3', a downstream primer is 5'-GCTGAATTGGCAGAGGAC-3', the probe is 5'-TTTTTCCCAGTTCACTGCCG-3', and the amplified target nucleotide sequence of the primer and probe are shown in SEQ ID No.3 in a sequence list; (2) is the sequence which is complementary to the sequence of (1). The specific primer and probe for real-time fluorescent PCR detection of the enterococcus faecalis can conduct qualitative and quantitative analysis on the clinically infected enterococcus faecalis content, has important significance on judging the clinically infected enterococcus faecalis content and plays an important role in the field of clinical tests.

The invention discloses a test strip for detecting 6-monoacetylmorphine as well as a preparation method and an application method of the test strip. The test strip sequentially comprises a sample absorption pad, a conjugate release pad, a reaction membrane, a water absorption pad and a bottom plate, an antibody marked by the time-resolved fluorescent microspheres is sprayed on the conjugate release pad, and the reaction membrane is coated with a detection line T of a hapten-carrier protein conjugate and a quality control line C of a goat-anti-mouse secondary antibody. According to the invention, the antibody is labeled by time-resolved fluorescent microspheres, and an immunochromatography technology is adopted to realize the rapid immunoassay of 6-monoacetylmorphine. The invention also provides a method for detecting the 6-monoacetylmorphine in a sample by using the above test strip. The test strip and the method have the advantages of being high in sensitivity and rapid in detection,being capable of achieving accurate quantification, being convenient to operate, being economical and practical, and being capable of achieving the rapid detection and on-site detection on a large batch of 6-monoacetylmorphine samples.

The invention discloses an ion chromatography method for simultaneously and quantitatively measuring the concentrations of 10 kinds of anions in rice. The 10 kinds of anions comprise F-, Ac-, Cl-, NO2, Br-, NO3-, SO4<2->, C2O4<2->, PO4<3->, and CrO4<2->. The concentrations and peak areas of F-, Ac-, Cl-, NO2, Br-, NO3-, SO4<2->, C2O4<2->, PO4<3->, and CrO4<2-> are quantified by an external standard method to show a strict linear relationship, the linearly dependent coefficient is more than 0.999, and the relative standard deviation is within 2.05%. The method is time-saving and efficient and can simultaneously quantify the 10 kinds of anions in rice, the peaks are attractive, the analysis results are accurate, the repeatability is high, derivation and gradient elution are not required, thepeak width and retention time are improved by column temperature, the 10 kinds of anions are well separated, the effect of temperature on the retention factor can be described by Van't Hoff equation,and an effective guidance can be provided for the analysis of rice ion composition and the nutrition and safety.

The invention discloses a test strip for detecting benzodiazepine and a preparation method and application method thereof. The test strip orderly comprises a sample absorption pad, a conjugate releasepad, a reaction film, a water absorbent pad and a bottom plate. The conjugate release pad is spray-coated with a time-resolved fluorescent microsphere-labeled antibody. The reaction film is coated with a hapten-carrier protein conjugate detection line and a goat anti-mouse secondary antibody quality control line. Through the time-resolved fluorescent microsphere-labeled antibody and immunochromatography, benzodiazepine can be fast analyzed. The invention also provides a method for detecting benzodiazepine in a sample through the test strip. The test strip has the advantages of high sensitivity, accurate quantification, rapid detection, convenient operation, economical and practical effects, and can realize rapid detection and on-site detection of a large amount of benzodiazepine samples.

The invention relates to a method for determining content of long-chain fatty alcohol in an organic phosphonic acid solution, and relates to the field of wet type metallurgy, which solves the technical problems of failure to simply and efficiently distinguish alcoholic hydroxyl group and phosphonate group and failure to monitor content of long-chain fatty alcohol in the additive in the existing chromatographic analysis method. The method has the advantages that by extracting the organic phase of an organic phosphate extracting agent, and quantitatively adding the long-chain fatty alcohol, the extracting property of the organic phosphonic acid extracting agent under different contents can be determined, the relationship among the extracting capacity, alcohol adding amount and organic phosphonic acid concentration is obtained, and the standard curve and equation are obtained; for the alcohol in the unknown system, the corresponding content of alcohol can be quantitatively obtained according to the extracting property by referring the standard curve and equation; the stability of the content of the long-chain fatty alcohol in the additive in the organic phase in the whole rare earth separation process can be monitored, the method is flexible and reliable, the accuracy is high, and the simple and rapid online monitoring, regulation and control can be truly realized.

The invention discloses a test strip for detecting amphetamine and a preparation method and application of the test strip. The test strip comprises a sample absorption pad, a conjugate release pad, areaction membrane, a water absorption pad and a substrate in sequence, wherein an antibody which is marked by time resolution fluorescent microspheres is sprayed to the conjugate release pad; a detection line of hapten-carrier protein conjugate and a quality control line of a sheep anti-rat secondary antibody are coated on the reaction membrane. By adopting the test strip, the antibody is marked by the time resolution fluorescent microspheres, immunochromatography assay is adopted, and rapid immunoassay of amphetamine can be achieved. The invention further provides a method for detecting amphetamine in a sample by using the test strip. The test strip has the advantages of being high in sensitivity, accurate in quantification, rapid in detection, convenient to operate and economic and practical, and rapid detection and on-site detection on large-scale amphetamine samples can be achieved.

The invention discloses an FITC test paper strip for detecting morphine as well as a preparation method and an application method thereof. The test paper strip comprises a sample absorption pad, a conjugate release pad, a reaction membrane, a water absorbing pad and a substrate in sequence, wherein the conjugate release pad is coated with an antibody marked by FITC; the reaction membrane is coatedwith a detection line of hapten-carrier protein conjugate and a quality control line of a goat-anti-mouse second antibody. Due to adoption of the FITC marked antibody and an immunochromatography technique, rapid immunoassay of morphine can be achieved. The invention further provides a method for detecting morphine in a sample by using the test paper strip. The test paper strip has the advantagesof being high in sensitivity, accurate in quantification, rapid in detection, convenient to operate, economical, and practical, and rapid detection and on-site detection on large scales of morphine samples can be achieved.

The invention belongs to the field of detection and analysis, and relates to a method for analyzing volatile aroma components contained in a blast bead filter stick. The method comprises the following steps of (1) crushing and/or puncturing blast beads in a blast bead filter stick sample, and then mixing with absolute ethyl alcohol; (2) detecting the mixture by adopting a headspace-gas chromatograph-mass spectrometer to obtain a detection spectrogram; (3) determining the volatile components contained in the blast bead filter stick through a spectrogram database and according to the detection spectrogram; (4) comparing the smell evaluation result of the volatile components with the smell evaluation result of the blast bead filter stick sample, and determining the volatile aroma components contained in the blast bead filter stick sample according to the comparison result; and (5) analyzing and quantifying the volatile aroma components in the blast bead filter stick through an internal standard and according to the detection spectrogram. According to the method, the volatile aroma components contained in the blast bead filter stick can be comprehensively analyzed, and the content of the volatile aroma components in the blast bead filter stick can be accurately quantified.

The invention relates to a duplex test strip for detecting methamphetamine and morphine as well as a preparation method and an application method of the duplex test strip. The test strip sequentiallycomprises a sample absorption pad, a conjugate release pad, a reaction membrane, a water absorption pad and a bottom plate, a methamphetamine antibody and a morphine antibody which are marked by the time-resolved fluorescent microspheres are sprayed on the conjugate release pad, and the reaction membrane is coated with a detection line T1 of a methylamphetamine hapten-carrier protein conjugate, adetection line T2 of a morphine hapten-carrier protein conjugate and a quality control line C of a goat anti-mouse secondary antibody (or Biotin-BSA). According to the invention, the antibodies are labeled by the time-resolved fluorescent microspheres, and the simultaneous and rapid immunoassay of methamphetamine and morphine is realized by adopting an immunochromatography technology. The invention also provides a method for simultaneously detecting the methamphetamine and morphine in a sample by using the above test strip. The duplex test strip and the method have the advantages of being highin sensitivity and rapid in detection, being capable of achieving accurate quantification, being convenient to operate, and being capable of achieving the rapid detection and on-site detection on a large batch of samples.

The present invention provides an extracting agent and a method for determining the free amine content in a spandex spinning raw solution. The extracting agent comprises a main reagent and a secondaryreagent, wherein the main reagent at least comprises a compound represented by the following formula (I), the secondary reagent is a compound having a hydrophilic end and a hydrophobic end in a molecular structure, the R in the formula (I) is defined in the specification, and the n in the R is an integer of 0-10, further preferably 0-5. According to the present invention, with the application ofthe extracting agent to extract the free amine in a spandex spinning raw solution, the free amine content can be easily and accurately quantified.