33results about How to "Accurate quantitative content" patented technology

The invention discloses an application of dual application reaction of Exo (exonuclease) III-assisted cycle and DNAzyme cycle to Hg<2+> detection. When Hg<2+> exists, T-rich parts of H-DNA (deoxyribonucleic acid) and A-DNA form a T-Hg<2+>-T metal base pair, and H-DNA and A-DNA are hybridized to initiate a chain replacement reaction to form a Hg<2+>-mediated double-chain structure (referred as to Hg-Complex) containing an overhung protection part and a DNAzyme part. Under the action of Exo III, Hg<2+> and A-DNA are released and are hybridized with another H-DNA for cycle to obtain a large amount of DNAzyme sequences. The obtained DNAzyme sequences can cyclically cut molecular beacons to generate amplified signals. By means of effective combination of two cycles, Hg<2+> in an aqueous solution can be detected sensitively by means of this strategy, and high selectivity is realized. In the meantime, the method can be used for detecting Hg<2+> in sewage successfully and provides a potential tool for quantitative detection of Hg<2+> in an environment water sample.

The invention discloses a water-soluble fertilizer capable of absorbing cadmium in foliage resistance and control paddy rice. The water-soluble fertilizer comprises the following components in percentages by weight: 17% to 22% of amino acids, 5% to 6% of polyglutamic acids, 19% to 28% of a metal chelate compound, 0.5% to 0.8% of a complexing agent and 5% to 8% of a surface active agent. The invention also discloses a preparation method of the water-soluble fertilizer. The preparation method comprises the following steps: (1) adding amino acids and polyglutamic acids into water, heating and stirring to obtain a mother solution A; (2) in a stirring condition, adding the metal chelate compound and the complexing agent into the mother solution A, and obtaining a mother solution B after complete dissolution; (3) in the stirring condition, adding the surface active agent into the mother solution B, and obtaining the water-soluble fertilizer product after complete dissolution. By using the water-soluble fertilizer, the cadmium content of paddy rice can be reduced, the cadmium content of paddy rice accords with the standard of edible safety, the production increase and quality increase ofpaddy rice are promoted, and secondary pollution to the environment is avoided.

The invention discloses a test paper strip for detecting tetrahydrocannabinolate as well as a preparation method and an application method thereof. The test paper strip comprises a sample absorption pad, a conjugate release pad, a reaction membrane, a water absorbing pad and a substrate in sequence, wherein the conjugate release pad is coated with an antibody marked by time resolved fluorescence microspheres; the reaction membrane is coated with a detection line of hapten-carrier protein conjugate and a quality control line of a goat-anti-mouse second antibody. Due to adoption of the time resolved fluorescence marked antibody and an immunochromatography technique, rapid immunoassay of tetrahydrocannabinolate can be achieved. The invention further provides a method for detecting tetrahydrocannabinolate in a sample by using the test paper strip. The test paper strip has the advantages of being high in sensitivity, accurate in quantification, rapid in detection, convenient to operate, economical, and practical, and rapid detection and on-site detection on large scales of tetrahydrocannabinolate samples can be achieved.

The invention relates to a triple test strip for detecting methamphetamine, morphine and ketamine as well as a preparation method and an application method of the triple test strip. The test strip sequentially comprises a sample absorption pad, a conjugate release pad, a reaction membrane, a water absorption pad and a bottom plate, a methamphetamine antibody, a morphine antibody and a ketamine antibody marked by the time-resolved fluorescent microspheres are sprayed on the conjugate release pad, and the reaction membrane is coated with a detection line T1 of a methylamphetamine antigen, a detection line T2 of a morphine antigen, a detection line T3 of a ketamine antigen and a quality control line C of a goat anti-mouse secondary antibody or Biotin-BSA. According to the immunochromatographytechnology, the simultaneous and rapid immunoassay of three drugs including methamphetamine, morphine and ketamine is realized. The invention also provides a method for simultaneously detecting the methamphetamine, morphine and ketamine in a sample by using the above test strip. The triple test strip and the method have the advantages of being high in sensitivity and rapid in detection, being capable of achieving accurate quantification, being convenient to operate, and being capable of achieving the rapid detection and on-site detection on a large batch of samples.

The invention discloses a test strip for detecting ketamine and a preparation method and application method thereof. The test strip orderly comprises a sample absorption pad, a conjugate release pad,a reaction film, a water absorbent pad and a bottom plate. The conjugate release pad is spray-coated with a time-resolved fluorescent microsphere-labeled antibody. The reaction film is coated with a hapten-carrier protein conjugate detection line and a goat anti-mouse secondary antibody quality control line. Through the time-resolved fluorescent microsphere-labeled antibody and immunochromatography, ketamine can be used for fast immunoassay. The invention also provides a method for detecting ketamine in a sample through the test strip. The test strip has the advantages of high sensitivity, accurate quantification, rapid detection, convenient operation, economical and practical effects, and can realize rapid detection and on-site detection of a large amount of ketamine samples.

The invention discloses an application of dual application reaction of Exo (exonuclease) III-assisted cycle and DNAzyme cycle to Hg<2+> detection. When Hg<2+> exists, T-rich parts of H-DNA (deoxyribonucleic acid) and A-DNA form a T-Hg<2+>-T metal base pair, and H-DNA and A-DNA are hybridized to initiate a chain replacement reaction to form a Hg<2+>-mediated double-chain structure (referred as to Hg-Complex) containing an overhung protection part and a DNAzyme part. Under the action of Exo III, Hg<2+> and A-DNA are released and are hybridized with another H-DNA for cycle to obtain a large amount of DNAzyme sequences. The obtained DNAzyme sequences can cyclically cut molecular beacons to generate amplified signals. By means of effective combination of two cycles, Hg<2+> in an aqueous solution can be detected sensitively by means of this strategy, and high selectivity is realized. In the meantime, the method can be used for detecting Hg<2+> in sewage successfully and provides a potential tool for quantitative detection of Hg<2+> in an environment water sample.

A multifunctional rapid food detector is provided with a motor used for driving upconversion fluorescence test strips to move, an adjustable-wavelength laser device module used for irradiating the upconversion fluorescence test strips, a bar code scanning module used for acquiring the ID identifier information of the upconversion fluorescence test strips, and an optical detection unit used for collecting visible light emitted by the upconversion fluorescence test strips. A unique ID identifier is set and attached to each upconversion fluorescence test strip, the signal input end of the motor and the signal input end of the adjustable-wavelength laser device module are connected with the control signal output end of a control unit, the signal output end of the bar code scanning module and the signal output end of the optical detection unit are connected with the signal input end of the control unit, the control unit is further connected with a cloud database through an internet module, the information output end of the control unit is connected with an SD memory card, and the information output end of the control unit is further connected with an LCD touch screen. The multifunctional rapid food detector has the advantages of being low in power consumption, high in precision and stability and suitable for detection occasions with various upconversion fluorescence test strips.

The invention discloses a method for determining the galactooligosaccharide content in milk powder, rice powder or milk and rice powder and application thereof.The method adopts an XAmide amide column and adopts ethanol and isopropyl alcohol according to the ratio of 1 to 4 in the pretreatment process of a sample to be detected to efficiently reduce lactose interference in the sample to be detected by being mixed with an organic solvent, the detection conditions are optimized, glucose, galactose and their epimers in an enzyme hydrolysis product are effectively separated, a linear regression equation is established, and the galactooligosaccharide content in the sample to be detected is accurately determined.The method is simple and easy to operate, the pretreatment time and costs are greatly reduced, a linear range is wide, and the method has the advantages of being low in interference, low in detection limit, high in accuracy and the like.

The invention discloses a method for detecting cytochrome c in a living cell based on a Raman-fluorescent dual-mode probe. The detecting method realizes dual-signal detection based on on-off conversion of Raman scattering and fluorescence resonance energy transfer through a nano-sensor which is constructed through assembling an adapter of the cytochrome c(Cyt c) on a gold triangle sheet (AuNTs). The method of the invention can simultaneously detect the Cyt c in the living cell through a Raman signal and a fluorescent signal. Furthermore the method has advantages of high detection singularity,high sensitivity and high accuracy. Furthermore compared with ELISA, the error is lower than 5%.

The invention provides a group of escherichia coli specific primers and probe sequences so as to establish a fluorescent quantitation PCR detection method which can be applied to detection of the content of escherichia coli in heart blood a dead rat and which is quick, sensitive and good in specificity. According to one side of the invention, specific primers and probes for detecting the escherichia coli are provided, and the specific primers and the probes use conservative fragment sequences as a target goal. A gene clone technique is adopted, escherichia coli LacZ gene specificity fragmentsare inserted into a carrier pMD18-T, so that recombinant plasmids containing LacZ gene specificity fragments are obtained and are used as standard products. A group of specific primers and probes aredesigned and synthesized according to escherichia coli LacZ gene specificity fragment sequences, the PCR reaction condition is optimized, a detection method by using a real-time fluorescent quantitation polymerase chain reaction as a platform is established, and the established method is evaluated.

The invention provides a specific primer and probe for real-time fluorescent PCR detection of enterococcus faecalis. The specific primer and probe for real-time fluorescent PCR detection of the enterococcus faecalis are characterized in that the nucleotide sequences of the specific primer and probe is one of (1) or (2), for (1), an upstream primer is 5'-TTCGCACCAGGAGATAAA-3', a downstream primer is 5'-GCTGAATTGGCAGAGGAC-3', the probe is 5'-TTTTTCCCAGTTCACTGCCG-3', and the amplified target nucleotide sequence of the primer and probe are shown in SEQ ID No.3 in a sequence list; (2) is the sequence which is complementary to the sequence of (1). The specific primer and probe for real-time fluorescent PCR detection of the enterococcus faecalis can conduct qualitative and quantitative analysis on the clinically infected enterococcus faecalis content, has important significance on judging the clinically infected enterococcus faecalis content and plays an important role in the field of clinical tests.

The invention discloses a test strip for detecting 6-monoacetylmorphine as well as a preparation method and an application method of the test strip. The test strip sequentially comprises a sample absorption pad, a conjugate release pad, a reaction membrane, a water absorption pad and a bottom plate, an antibody marked by the time-resolved fluorescent microspheres is sprayed on the conjugate release pad, and the reaction membrane is coated with a detection line T of a hapten-carrier protein conjugate and a quality control line C of a goat-anti-mouse secondary antibody. According to the invention, the antibody is labeled by time-resolved fluorescent microspheres, and an immunochromatography technology is adopted to realize the rapid immunoassay of 6-monoacetylmorphine. The invention also provides a method for detecting the 6-monoacetylmorphine in a sample by using the above test strip. The test strip and the method have the advantages of being high in sensitivity and rapid in detection,being capable of achieving accurate quantification, being convenient to operate, being economical and practical, and being capable of achieving the rapid detection and on-site detection on a large batch of 6-monoacetylmorphine samples.

The invention discloses an ion chromatography method for simultaneously and quantitatively measuring the concentrations of 10 kinds of anions in rice. The 10 kinds of anions comprise F-, Ac-, Cl-, NO2, Br-, NO3-, SO4<2->, C2O4<2->, PO4<3->, and CrO4<2->. The concentrations and peak areas of F-, Ac-, Cl-, NO2, Br-, NO3-, SO4<2->, C2O4<2->, PO4<3->, and CrO4<2-> are quantified by an external standard method to show a strict linear relationship, the linearly dependent coefficient is more than 0.999, and the relative standard deviation is within 2.05%. The method is time-saving and efficient and can simultaneously quantify the 10 kinds of anions in rice, the peaks are attractive, the analysis results are accurate, the repeatability is high, derivation and gradient elution are not required, thepeak width and retention time are improved by column temperature, the 10 kinds of anions are well separated, the effect of temperature on the retention factor can be described by Van't Hoff equation,and an effective guidance can be provided for the analysis of rice ion composition and the nutrition and safety.

The invention provides a shrimp immunofluorescence detection test strip and application, and relates to the technical field of detection test strips. The immunofluorescence detection test strip comprises a sample pad, a conjugate pad, a nitrocellulose membrane and absorbent paper which are sequentially assembled and adhered to a PVC bottom plate, wherein the conjugate pad is coated with a fluorescent latex microsphere labeled mixed antibody, the nitrocellulose membrane is sequentially coated with an antigen myosin antibody (T1 line), an anti-arginine kinase antibody (T2 line), an anti-hemocyanin antibody (T3 line), an anti-troponin / myosin light chain antibody (T4 line) and a rabbit anti-mouse IgG antibody (C line), the T1 line, T2 line, T3 line and T4 line are quality control lines, and the line C is a quality control line. The test strip can accurately and quantitatively detect the content of tropomyosin, arginine kinase, hemocyanin and troponin / tropomyosin light chains in food, and is simple and convenient to operate, high in accuracy and high in sensitivity.

The invention relates to the technical field of analytical chemistry, in particular to an analysis method for separating and accurately determining rivaroxaban impurities by adopting high performance liquid chromatography. On the basis of high performance liquid chromatography, the method adopts proper chromatographic conditions and ensures the separation degree of all impurities; and for the impurity N and the impurity K which are difficult to separate under the conditions, the two impurities difficult to separate are accurately quantified through a double-slope-length mode and by combining peak areas of the two impurities under two specific wavelengths and a correction factor relative to a main peak.

The invention discloses a test strip for detecting benzodiazepine and a preparation method and application method thereof. The test strip orderly comprises a sample absorption pad, a conjugate releasepad, a reaction film, a water absorbent pad and a bottom plate. The conjugate release pad is spray-coated with a time-resolved fluorescent microsphere-labeled antibody. The reaction film is coated with a hapten-carrier protein conjugate detection line and a goat anti-mouse secondary antibody quality control line. Through the time-resolved fluorescent microsphere-labeled antibody and immunochromatography, benzodiazepine can be fast analyzed. The invention also provides a method for detecting benzodiazepine in a sample through the test strip. The test strip has the advantages of high sensitivity, accurate quantification, rapid detection, convenient operation, economical and practical effects, and can realize rapid detection and on-site detection of a large amount of benzodiazepine samples.

The invention relates to a method for determining content of long-chain fatty alcohol in an organic phosphonic acid solution, and relates to the field of wet type metallurgy, which solves the technical problems of failure to simply and efficiently distinguish alcoholic hydroxyl group and phosphonate group and failure to monitor content of long-chain fatty alcohol in the additive in the existing chromatographic analysis method. The method has the advantages that by extracting the organic phase of an organic phosphate extracting agent, and quantitatively adding the long-chain fatty alcohol, the extracting property of the organic phosphonic acid extracting agent under different contents can be determined, the relationship among the extracting capacity, alcohol adding amount and organic phosphonic acid concentration is obtained, and the standard curve and equation are obtained; for the alcohol in the unknown system, the corresponding content of alcohol can be quantitatively obtained according to the extracting property by referring the standard curve and equation; the stability of the content of the long-chain fatty alcohol in the additive in the organic phase in the whole rare earth separation process can be monitored, the method is flexible and reliable, the accuracy is high, and the simple and rapid online monitoring, regulation and control can be truly realized.

The invention discloses a test strip for detecting amphetamine and a preparation method and application of the test strip. The test strip comprises a sample absorption pad, a conjugate release pad, areaction membrane, a water absorption pad and a substrate in sequence, wherein an antibody which is marked by time resolution fluorescent microspheres is sprayed to the conjugate release pad; a detection line of hapten-carrier protein conjugate and a quality control line of a sheep anti-rat secondary antibody are coated on the reaction membrane. By adopting the test strip, the antibody is marked by the time resolution fluorescent microspheres, immunochromatography assay is adopted, and rapid immunoassay of amphetamine can be achieved. The invention further provides a method for detecting amphetamine in a sample by using the test strip. The test strip has the advantages of being high in sensitivity, accurate in quantification, rapid in detection, convenient to operate and economic and practical, and rapid detection and on-site detection on large-scale amphetamine samples can be achieved.

The invention discloses an FITC test paper strip for detecting morphine as well as a preparation method and an application method thereof. The test paper strip comprises a sample absorption pad, a conjugate release pad, a reaction membrane, a water absorbing pad and a substrate in sequence, wherein the conjugate release pad is coated with an antibody marked by FITC; the reaction membrane is coatedwith a detection line of hapten-carrier protein conjugate and a quality control line of a goat-anti-mouse second antibody. Due to adoption of the FITC marked antibody and an immunochromatography technique, rapid immunoassay of morphine can be achieved. The invention further provides a method for detecting morphine in a sample by using the test paper strip. The test paper strip has the advantagesof being high in sensitivity, accurate in quantification, rapid in detection, convenient to operate, economical, and practical, and rapid detection and on-site detection on large scales of morphine samples can be achieved.

The invention provides a milk immunofluorescence detection test strip and application, and relates to the technical field of detection test strips. The immunofluorescence detection test strip comprises a sample pad, a conjugate pad, a nitrocellulose membrane and absorbent paper which are sequentially assembled and adhered to a PVC bottom plate; the conjugate pad is coated with a fluorescent latexmicrosphere labeled mixed antibody, the nitrocellulose membrane is sequentially coated with an anti-casein antibody (T1 line), an anti-beta lactoglobulin antibody (T2 line), an anti-alpha whey proteinantibody (T3 line), an anti-lactoferrin / bovine serum albumin antibody (T4 line) and a rabbit anti-mouse IgG antibody (C line), and the T1 line, T2 line, T3 line and T4 line are quality control lines.The method can be used for accurately and quantitatively detecting the contents of casein, anti-beta lactoglobulin, anti-alpha whey protein and anti-lactoferrin / bovine serum albumin in food, and is simple and convenient to operate, high in accuracy and high in sensitivity.

The invention belongs to the field of detection and analysis, and relates to a method for analyzing volatile aroma components contained in a blast bead filter stick. The method comprises the following steps of (1) crushing and / or puncturing blast beads in a blast bead filter stick sample, and then mixing with absolute ethyl alcohol; (2) detecting the mixture by adopting a headspace-gas chromatograph-mass spectrometer to obtain a detection spectrogram; (3) determining the volatile components contained in the blast bead filter stick through a spectrogram database and according to the detection spectrogram; (4) comparing the smell evaluation result of the volatile components with the smell evaluation result of the blast bead filter stick sample, and determining the volatile aroma components contained in the blast bead filter stick sample according to the comparison result; and (5) analyzing and quantifying the volatile aroma components in the blast bead filter stick through an internal standard and according to the detection spectrogram. According to the method, the volatile aroma components contained in the blast bead filter stick can be comprehensively analyzed, and the content of the volatile aroma components in the blast bead filter stick can be accurately quantified.

The invention relates to a duplex test strip for detecting methamphetamine and morphine as well as a preparation method and an application method of the duplex test strip. The test strip sequentiallycomprises a sample absorption pad, a conjugate release pad, a reaction membrane, a water absorption pad and a bottom plate, a methamphetamine antibody and a morphine antibody which are marked by the time-resolved fluorescent microspheres are sprayed on the conjugate release pad, and the reaction membrane is coated with a detection line T1 of a methylamphetamine hapten-carrier protein conjugate, adetection line T2 of a morphine hapten-carrier protein conjugate and a quality control line C of a goat anti-mouse secondary antibody (or Biotin-BSA). According to the invention, the antibodies are labeled by the time-resolved fluorescent microspheres, and the simultaneous and rapid immunoassay of methamphetamine and morphine is realized by adopting an immunochromatography technology. The invention also provides a method for simultaneously detecting the methamphetamine and morphine in a sample by using the above test strip. The duplex test strip and the method have the advantages of being highin sensitivity and rapid in detection, being capable of achieving accurate quantification, being convenient to operate, and being capable of achieving the rapid detection and on-site detection on a large batch of samples.

The invention relates to a method for determining content of long-chain fatty alcohol in an organic phosphonic acid solution, and relates to the field of wet type metallurgy, which solves the technical problems of failure to simply and efficiently distinguish alcoholic hydroxyl group and phosphonate group and failure to monitor content of long-chain fatty alcohol in the additive in the existing chromatographic analysis method. The method has the advantages that by extracting the organic phase of an organic phosphate extracting agent, and quantitatively adding the long-chain fatty alcohol, the extracting property of the organic phosphonic acid extracting agent under different contents can be determined, the relationship among the extracting capacity, alcohol adding amount and organic phosphonic acid concentration is obtained, and the standard curve and equation are obtained; for the alcohol in the unknown system, the corresponding content of alcohol can be quantitatively obtained according to the extracting property by referring the standard curve and equation; the stability of the content of the long-chain fatty alcohol in the additive in the organic phase in the whole rare earth separation process can be monitored, the method is flexible and reliable, the accuracy is high, and the simple and rapid online monitoring, regulation and control can be truly realized.

The invention provides a joint detection kit for procalcitonin, interleukin 6 and heparin binding protein, and belongs to the technical field of in-vitro diagnosis. The joint detection kit comprises a test strip, and the test strip comprises a substrate, and a sample pad, a CP pad, an NC membrane and an absorption pad which are sequentially pasted on the substrate. The CP pad is coated with a fluorescein labeled conjugate of a PCT antibody, an IL-6 antibody, an HBP antibody and chicken IgY; a detection line coated with a PCT monoclonal antibody, an IL-6 monoclonal antibody and an HBP monoclonal antibody and a quality control line coated with a rabbit anti-chicken IgY monoclonal antibody are respectively arranged on the NC membrane. According to the kit disclosed by the invention, the procalcitonin, the interleukin 6 and the heparin binding protein can be accurately and quantitatively detected at high sensitivity through one-time sampling.

The present invention provides an extractant and a method for measuring the content of free amine in spandex spinning stock solution. The extractant provided by the present invention includes a main reagent and a secondary reagent, and the main reagent includes the compound shown in the following formula (I): At least one, the secondary reagent is a compound with a hydrophilic end and a hydrophobic end in the molecular structure: wherein, R in the formula (I) is, n in R is an integer of 0-10, and n is further preferably 0-5 an integer of . Utilizing the extractant of the invention to extract the free amine in the spandex spinning dope is beneficial to accurately quantify the content of the free amine therein.

The invention relates to a real-time fluorescence quantitative PCR kit for detecting Enterobacter aerogenes, which comprises a PCR reaction liquid, an enzyme mixed liquid, a Cad gene standard substance of Enterobacter aerogenes, a negative control substance and a positive control substance. The present invention can achieve the purpose of accurately quantifying the content of Enterobacter aerogenes in the specimen to be tested by extracting the genomic DNA of Enterobacter aerogenes and combining the real-time fluorescent quantitative PCR detection technology, which has the characteristics of fast, sensitive and specificity, and is very useful for judging gas production. The content of Enterobacteriaceae active bacteria and the follow-up study of urinary tract infection are of great significance.

The present invention provides an extracting agent and a method for determining the free amine content in a spandex spinning raw solution. The extracting agent comprises a main reagent and a secondaryreagent, wherein the main reagent at least comprises a compound represented by the following formula (I), the secondary reagent is a compound having a hydrophilic end and a hydrophobic end in a molecular structure, the R in the formula (I) is defined in the specification, and the n in the R is an integer of 0-10, further preferably 0-5. According to the present invention, with the application ofthe extracting agent to extract the free amine in a spandex spinning raw solution, the free amine content can be easily and accurately quantified.

The invention discloses an FITC test paper strip for detecting amphetamine as well as a preparation method and an application method thereof. The test paper strip comprises a sample absorption pad, aconjugate release pad, a reaction membrane, a water absorbing pad and a substrate in sequence, wherein the conjugate release pad is coated with an antibody marked by FITC; the reaction membrane is coated with a detection line of hapten-carrier protein conjugate and a quality control line of a goat-anti-mouse second antibody. Due to adoption of the FITC marked antibody and an immunochromatography technique, rapid immunoassay of amphetamine can be achieved. The invention further provides a method for detecting amphetamine in a sample by using the test paper strip. The test paper strip has the advantages of being high in sensitivity, accurate in quantification, rapid in detection, convenient to operate, economical, and practical, and rapid detection and on-site detection on large scales of amphetamine samples can be achieved.

The invention discloses a method for determining the galactooligosaccharide content in milk powder, rice powder or milk and rice powder and application thereof.The method adopts an XAmide amide column and adopts ethanol and isopropyl alcohol according to the ratio of 1 to 4 in the pretreatment process of a sample to be detected to efficiently reduce lactose interference in the sample to be detected by being mixed with an organic solvent, the detection conditions are optimized, glucose, galactose and their epimers in an enzyme hydrolysis product are effectively separated, a linear regression equation is established, and the galactooligosaccharide content in the sample to be detected is accurately determined.The method is simple and easy to operate, the pretreatment time and costs are greatly reduced, a linear range is wide, and the method has the advantages of being low in interference, low in detection limit, high in accuracy and the like.