Phaseolus vulgaris L. germplasm improvement method
A technology of kidney bean and germplasm, applied in the direction of plant genetic improvement, botany equipment and methods, application, etc., can solve the problems of low success rate and high requirements for operators, and achieve simple operation, fast and accurate detection, and high hybridization success rate Effect
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Embodiment 1
[0036] Select the bean varieties SJ-0009 and SJ-0206, arrange the sowing in a random block design, repeat 3 times, routinely fertilize the base soil, plan the planting area reasonably, arrange the sowing after mulching, and carry out cultivation management with local conventional techniques;
[0037] Take the flower buds of SJ-0009 that turn from green to white, use tweezers to gently push the flag petals and turn them over, tear off the upper part of the keel petals, remove the anthers around the stigma, squeeze the anthers of SJ-0206 with tweezers, and remove the pollen Gently wipe on the SJ-0009 stigma, restore the flipped flag petals to their original shape, gently wrap the flower buds with a small piece of toilet paper, try not to touch the flower buds directly, fix them with paper clips, remove the rest of the surrounding flowers and flower buds, and write Labels with the names of the parents and the date of hybridization are hung on the stalks of the inflorescences. Afte...
Embodiment 2
[0041] Select the bean varieties SJ-0021 and SJ-0119, arrange the sowing in a random block design, repeat 3 times, routinely fertilize the base soil, plan the planting area reasonably, arrange the sowing after mulching, and carry out cultivation management with local conventional techniques;
[0042]Take the flower buds of SJ-0021 that turn from green to white, use tweezers to gently push the flag petals and turn them over, tear off the upper part of the keel petals, remove the anthers around the stigma, squeeze the anthers of SJ-0119 with tweezers, and remove the pollen Gently wipe it on the SJ-0021 stigma, restore the flipped flag petals to their original shape, wrap the flower buds gently with a small section of toilet paper, try not to touch the flower buds directly, fix them with paper clips, remove the remaining flowers and flower buds around, and write Labels with the names of the parents and the date of hybridization are hung on the stalks of the inflorescences. After o...
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