Phaseolus vulgaris L. germplasm improvement method
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A technology of kidney bean and germplasm, applied in the direction of plant genetic improvement, botany equipment and methods, application, etc., can solve the problems of low success rate and high requirements for operators, and achieve simple operation, fast and accurate detection, and high hybridization success rate Effect
Pending Publication Date: 2020-04-10
JIANGHAN UNIVERSITY
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[0004] At present, there is no report on low-PHA kidney bean germplasm, and the success rate of common kidney bean hybridization methods is extremely low, and the requirements for operators are relatively high
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Embodiment 1
[0036] Select the bean varieties SJ-0009 and SJ-0206, arrange the sowing in a random block design, repeat 3 times, routinely fertilize the base soil, plan the planting area reasonably, arrange the sowing after mulching, and carry out cultivation management with local conventional techniques;
[0037] Take the flower buds of SJ-0009 that turn from green to white, use tweezers to gently push the flag petals and turn them over, tear off the upper part of the keel petals, remove the anthers around the stigma, squeeze the anthers of SJ-0206 with tweezers, and remove the pollen Gently wipe on the SJ-0009 stigma, restore the flipped flag petals to their original shape, gently wrap the flower buds with a small piece of toilet paper, try not to touch the flower buds directly, fix them with paper clips, remove the rest of the surrounding flowers and flower buds, and write Labels with the names of the parents and the date of hybridization are hung on the stalks of the inflorescences. Afte...
Embodiment 2
[0041] Select the bean varieties SJ-0021 and SJ-0119, arrange the sowing in a random block design, repeat 3 times, routinely fertilize the base soil, plan the planting area reasonably, arrange the sowing after mulching, and carry out cultivation management with local conventional techniques;
[0042]Take the flower buds of SJ-0021 that turn from green to white, use tweezers to gently push the flag petals and turn them over, tear off the upper part of the keel petals, remove the anthers around the stigma, squeeze the anthers of SJ-0119 with tweezers, and remove the pollen Gently wipe it on the SJ-0021 stigma, restore the flipped flag petals to their original shape, wrap the flower buds gently with a small section of toilet paper, try not to touch the flower buds directly, fix them with paper clips, remove the remaining flowers and flower buds around, and write Labels with the names of the parents and the date of hybridization are hung on the stalks of the inflorescences. After o...
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Abstract
The invention provides a phaseolus vulgaris L. germplasm improvement method. The method comprises the following steps: 1) sowing seeds of phaseolus vulgaris L. varieties with low PHA (phytohemagglutinin) contents or excellent economical characters, and performing planting management; 2) performing hybridization on a phaseolus vulgaris L. variety with the low PHA content as a female parent and a phaseolus vulgaris L. variety with excellent economical characters as a male parent in the step 1), and collecting seeds; 3) sowing the collected seeds in a single plant sowing manner, and performing planting management; 4) calculating the content of PHA in commercial mature pods of phaseolus vulgaris L. planted in the step 3); 5) collecting seeds obtained in the step 3), screening a strain with low PHA according to results of the step 4), sowing the phaseolus vulgaris L. seeds with low PHA in a single plant sowing manner, and performing planting management; and 6) repeating the step 4) and thestep 5) till a fifth to sixth strain, so as to obtain germplasm with the low PHA content. The method provided by the invention firstly achieves innovation of the germplasm of the phaseolus vulgaris L. with low PHA, and in addition, has the advantages of being high in hybridization success rate, simple and easy to operate, rapid and accurate in agglutinin content detection, applicable to large-scale testing, and the like.
Description
technical field [0001] The invention belongs to the field of plant germplasm innovation, and in particular relates to a method for improving kidney bean germplasm. Background technique [0002] Kidney bean (Phaseolus vulgaris L.) is an annual herbaceous plant belonging to Leguminosa and Papilionideae. Kidney beans have high nutritional value and rich protein content. Kidney beans are not only widely cultivated in various parts of my country, but are also planted as a major source of plant protein in many parts of Asia, Africa and Latin America. The planting area in the world is second only to soybeans. Edible beans are an important crop worldwide. [0003] Food poisoning incidents caused by eating undercooked kidney beans have occurred from time to time, casting a shadow over the development of the kidney bean industry. Studies have shown that the main toxin in kidney beans is phytohemagglutinin (PHA). How to reduce or remove PHA in kidney beans is the primary problem to s...
Claims
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