A kind of anthurium hybrid breeding and rapid propagation method
A hybrid breeding and anthurium technology, applied in horticultural methods, botanical equipment and methods, plant genetic improvement, etc., can solve the problems of low heterozygous rate, low seed germination rate, long breeding cycle, etc. of anthurium, and achieve successful hybridization. The effect of high rate, good growth and good seedling quality
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Embodiment 1
[0065] Step 1: material selection
[0066] Choose Dakota and Cherry red, and take Dakota as the female parent and Cherry red as the male parent.
[0067] Step 2: Manual pollination
[0068] During the flowering period from December to February, collect pollen when the anther sac of the cherry red spadix is cracked for 1 to 2 days, and use a brush to impart it to the mucus secreted at the base of the spadix of the female parent. After pollination, bagging, labeling, indicating the parents, hybridization date and other information, and collecting as explants when the seeds mature to yellow;
[0069] After 4 months, the success rate of pollination was counted, reaching more than 90%.
[0070] Step 3: Aseptic sowing culture
[0071] Rinse the dirt on the surface of the yellow seeds with washing powder, then remove and clean the outer peel, soak in 75% alcohol for 35 seconds, then disinfect with 0.15% mercuric solution for 15 minutes, rinse with sterile water 6 times, filter p...
Embodiment 2
[0083] Step 1: material selection
[0084] Choose Baby pear and Princess Amalia Brilliant, and use Baby pear as the female parent and Dream as the male parent.
[0085] Step 2: Manual pollination
[0086] Since the flowering period of Polaroid and Dream is different, the parent material of the male parent Dream is placed in an artificial climate chamber, in which the temperature is set at 16°C at night, the cultivation temperature is 28°C-32°C during the day, the light intensity is 2000lx, and the light duration is 12h / d; one plant is treated Month; pollen was collected when the anther sac of the dreamy spadix cracked for 1 to 2 days, and was transferred to the mucus secreted by the base of the spadix of the female parent Polaroid with a brush. After pollination, bagging, labeling, indicating the parents, hybridization date and other information, and collecting as explants when the seeds mature to yellow;
[0087] After 4 months, the success rate of pollination was counted, ...
Embodiment 3
[0101] Step 1: material selection
[0102] Select Pandola (Bandola) and Yunzhi (Yunzhi), and use Pandora as the female parent and Yunzhi as the male parent.
[0103] Step Two: Pollination
[0104] Because Pandora and Versicolor versicolor have different flowering periods, the parent material of Versicolor versicolor was placed in an artificial climate chamber, where the temperature was set at 18°C at night, the cultivation temperature during the day was 28°C-32°C, the light intensity was 2000lx, and the light duration was 12h / d; the treatment was 45 days; The pollen was collected when the pollen sac of Versicolor versicolor red spadix cracked for 1 to 2 days, and was transferred to the mucus at the base of the spadix of the female parent Big Brother with a brush. After pollination, bagging, labeling, indicating the parents, hybridization date and other information, and collecting as explants when the seeds mature to yellow;
[0105] After 4 months, the success rate of poll...
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