Method for hybridizing cymbidium goeringii, aseptically sowing seeds and raising seedlings

Abstract

The invention provides a method for hybridizing cymbidium goeringii, aseptically sowing seeds and raising seedlings, which comprises the following steps of: performing artificial pollination to obtain hybrid seeds; picking off the capsule when the capsule has not reached full maturity; disinfecting the surface of the capsule and aseptically sowing the capsule; culturing the capsule in the dark and sprouting to obtain the rhizome; inoculating the rhizome into a bud induction culture medium; inducing bud through the rhizome, rooting and culturing the sprout; and transplanting test-tube plantletand the like. The method ensures high germination of the cymbidium goeringii hybrid seeds, short period for raising the seedlings and good quality of the seedlings by using three culture media optimized by sifting. The complete set of method of the invention has high operability, well solves the problems of extremely difficult germination of the cymbidium goeringii hybrid seeds under natural conditions due to the incomplete embryonic development and has high application value and popularization value.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, in particular to a method for hybrid breeding and seed propagation of Chunlan. Background technique [0002] Orchid is a traditional characteristic flower in China. Chinese orchid usually refers to a part of the ground species in the Orchidaceae (Orchidaceae) of the genus Cymbidum, such as Chunlan (C.goeringii), Cymbidium (C.faberi), Jianlan (C. ensifolium), Molan (C.sinense) and Hanlan (C.kanran), etc. Orchids are elegant and fragrant, highly praised in poems and paintings, and known as "a gentleman among flowers". Confucian culture advocates "a gentleman is better than virtue", and flowers are often used as metaphors, making orchids very popular in Southeast Asian countries. The quantity of fine orchid varieties is small and the price is high, and new varieties are mainly obtained by introduction and domestication. Since the 1980s, various places have taken advantage of local resources to ca...

AI Technical Summary

Problems solved by technology

In the past ten years, researches on cross-breeding of local orchid resources have been carried out in China, such as Molan in Guangdong, Jianlan and Hanlan in Fujian, Chunjian in Sichuan, and Lianpetalan in Yunnan. There are thousands of seeds in Lishuo fruit, but the seeds have no endosperm and cotyledons, and there is only an immature tadpole-shaped embryo in the transparent seed coat. It is difficult to germinate in conventional sowing, so it must be carried out by tissue culture aseptic sowing. sprout

The research on crossbreeding of national orchids in various places has obvious regional characteristics, and some of them are mainly based on scientific research. Most of the parents selected are natural hybrids or self-crosses of common grass orchids, which are not conducive to the acquisition of excellent offspring.

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