A rapid, efficient and non-destructive method for identifying high-lysine waxy corn kernels
A high-lysine waxy and waxy corn technology, which is applied in botany equipment and methods, plant genetic improvement, application, etc., can solve the problems of waste, high cost, and more land resources, and achieve time saving and accuracy High, save the effect of testing manpower, material resources and capital investment
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Embodiment 1
[0033] 1. Hybridization
[0034] Jingnuo 6, the backbone inbred line of waxy maize in my country, was selected and provided by the Maize Research Center of Beijing Academy of Agriculture and Forestry Sciences as the female parent, with a lysine content of 0.33%. The maize germplasm material A containing opaque-2 was used as the male parent, whose genotype was homozygous recessive (o2o2), and the lysine content in the grain was 0.48% (the lysine content in the corn grain ≥ 0.4% means high lysine Acid corn), crossed to obtain the F1 generation.
[0035] 2. Acquisition of waxy corn seeds
[0036] (1) Selfing
[0037] The F1 generation seeds in the above 1 were planted and strictly selfed, and the pollination date was recorded. Harvest at the full maturity stage of the grains to obtain F2 generation seeds.
[0038] (2) Backcross
[0039] The seeds of the F1 generation in the above 1 were planted, the F1 plants were used as the female parent, and Jingnuo 6 was used as the male...
Embodiment 2
[0053] 1. Hybridization
[0054] Jingnuo 6, the backbone inbred line of waxy maize in my country, was selected and provided by the Maize Research Center of Beijing Academy of Agriculture and Forestry Sciences as the female parent, with a lysine content of 0.33%. The maize germplasm material A containing opaque-2 was used as the male parent, whose genotype was homozygous recessive (o2o2), and the lysine content in the grain was 0.48% (the lysine content in the corn grain ≥ 0.4% means high lysine Acid corn), crossed to obtain the F1 generation.
[0055] 2. Acquisition of waxy corn seeds
[0056] The seeds of the F1 generation in the above 1 were planted, the F1 plants were used as the female parent, and Jingnuo 6 was used as the male parent for backcrossing, and the pollination date was recorded. Harvested at the full maturity stage of the grains to obtain BC1F1 generation seeds. According to the phenotype, the opaque seeds are selected for next generation planting.
[0057]...
Embodiment 3
[0059] Example 3 Comparison of germplasm characteristics of high lysine waxy maize at different developmental stages
[0060] 1. Hybridization
[0061] Jingnuo 6, the backbone inbred line of waxy maize in my country, was selected and provided by the Maize Research Center of Beijing Academy of Agriculture and Forestry Sciences as the female parent, with a lysine content of 0.33%. The maize germplasm material A containing opaque-2 was used as the male parent, whose genotype was homozygous recessive (o2o2), and the lysine content in the grain was 0.48% (the lysine content in the corn grain ≥ 0.4% means high lysine Acid corn), crossed to obtain the F1 generation.
[0062] 2. Acquisition of waxy corn seeds
[0063] The seeds of the F1 generation in the above 1 were planted, the F1 plants were used as the female parent, and Jingnuo 6 was used as the male parent for backcrossing, and the pollination date was recorded. Harvested at the full maturity stage of the grains to obtain BC...
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