Material culture method for preparing sugarcane wild species chromosome sample
A technology of sample preparation and culture method, which is applied in the field of material culture for the preparation of wild sugarcane chromosome samples, can solve the problems of difficult collection of root tips, old root tips, and small number, and achieve variety improvement, development promotion, and root growth. Effect
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Embodiment 1
[0034] Such as Figure 1-2 Shown:
[0035] A material culture method for preparing wild sugarcane chromosome samples, comprising the following steps:
[0036] 1) dig out the sugarcane wild resource clone material from the resource garden, the material has tiller bud points and 3-5 old roots, and cut off all the leaves of the material;
[0037] 2) Fill the liner 2 of the special potted culture pot 1 with impurity-free soil to 1 / 3 of the total volume, plant the sample in the liner 2 and cover with soil to 1 / 2 of the total volume, water thoroughly and cover with transparent Place the plastic film in a greenhouse at about 30°C for cultivation to allow the seedlings to germinate;
[0038] 3) When the material grows 8-12 tillers and the height reaches 75-85cm, lift the liner 2 together with the soil and the material plant and take it out from the potted culture pot 1;
[0039] 4) Fill the potted culture pot 1 with 1:3 mixed perlite and sun-dried lawn grass to 1 / 3-1 / 2 of the volum...
Embodiment 2
[0046] Such as Figure 1-2 Shown: a potted culture pot for preparing wild sugarcane chromosome sample materials, including a potted culture pot 1 and an inner tank 2, the potted culture pot 1 is a cylindrical pot body, the lower end is equipped with a base 6 and has a through hole, and is placed side by side Place the liner 2 in the potted culture pot 1 on the pot mat 3, the inner pot 2 is a hollow cylinder with a diameter 1-2 cm smaller than the potted culture pot 1, a cross-shaped support 4 and a grid cloth 5 are installed at the bottom, and the upper end is open And install a handle that is symmetrical to the center;
[0047] The potted culture pot 1 and the inner wall of the liner 2 are all designed with a scale line 7;
[0048] The potted culture pot 1 is a pottery pot, and the liner 2 is made of plastic with a thickness of 0.3-0.5 cm.
Embodiment 3
[0050] Such as Figure 1-2 Shown: the application of a special pot culture pot for preparing chromosome sample material of wild sugarcane species in the cultivation of somatic cell chromosome material of wild sugarcane root tip, including:
[0051] After placing the liner 2 in the potted culture pot 1, fill 1 / 3 of the impurity-free soil in the liner 2, plant the prepared sample on the soil and cover the soil to 1 / 2 of the total volume, water thoroughly and cover After the transparent plastic film is placed in a greenhouse at about 30°C, the seedlings will germinate;
[0052] When the material grows 8-12 tillers and the height reaches 75-85cm, the inner container is taken out from the potted culture pot 1 through the handle on the inner container 2, and the soil and material plants in the inner container 2 are completely preserved;
[0053] Fill the potted culture pot 1 with 1:3 mixed perlite and dried lawn grass to 1 / 3-1 / 2 of the volume of the pot, and replant the liner 2 of ...
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