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Panda milk preservation method

A preservation method and technology of giant pandas, which are applied in the preservation, application, and animal husbandry of human or animal bodies, can solve problems such as short preservation time, low milk cell viability, and influence of cells in milk, so as to improve cell Viability, effects on healthy growth and development

Inactive Publication Date: 2020-04-21
CHENGDU RES BASE OF GIANT PANDA BREEDING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, conventional milk preservation methods include cold storage and cryopreservation, but these conventional preservation methods have some problems in the preservation of giant panda milk
For example, refrigerated storage at 4°C can better preserve the main nutrients of milk, but the storage time is short, generally only about 3 days, which is suitable for short-term storage; direct frozen storage, such as -20°C, -80°C or -196 ℃ freezing, it can be stored for a long time, but this kind of preservation can only preserve the nutrients in the milk, and the cell viability in the milk has no advantage
However, there is no report on the effect of breast milk cryopreservation on the cells in milk

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for preserving giant panda milk. Take 0.5ml of giant panda milk respectively, put them into 2ml cryopreservation tubes respectively, then place the cryopreservation tubes in a programmed cooling box at room temperature, and then store the giant panda milk at -80 ℃ for 24 hours, and put it into liquid nitrogen immediately after taking out the programmed cooling box.

[0024] Among them, the program cooling box used is For LX freezer, the cooling rate is -1°C / min.

Embodiment 2

[0026] A method for preserving giant panda milk. Take 1ml of giant panda milk respectively, put them into 2ml cryopreservation tubes, then place the cryopreservation tubes in a programmed cooling box at room temperature, and then store the giant panda milk at -80°C. Place it for 24 hours, take out the programmed cooling box and immediately put it into liquid nitrogen for storage. Among them, the program cooling box used is For LX freezer, the cooling rate is -1°C / min.

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PUM

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Abstract

The invention discloses a panda milk preservation method. The method comprises the following steps: storing panda milk at -80 DEG C for 24 h, and then putting and storing the panda milk in liquid nitrogen. The preservation method makes the panda milk preserved for a long time, and results of analysis show that the panda milk preserved by using the method can effectively improve the cell viabilityin the panda milk, and the preservation method is more beneficial to healthy growth and development of young pandas compared with a conventional storage mode.

Description

technical field [0001] The invention relates to a method for preserving animal milk, in particular to a method for preserving giant panda milk. Background technique [0002] The giant panda (Ailuropoda melanoleuca) is a rare animal unique to my country. Improving the success rate of giant panda breeding and raising young has always been the focus of captive giant panda conservation work, and it is also a difficult research point. Giant panda cubs are born prematurely, and their later development is completely dependent on breastfeeding. Breastfeeding of giant panda cubs plays a very important role in the survival rate and healthy growth of giant panda cubs. Because the composition of giant panda breast milk is complex and diverse, and has many biological functions, it not only provides nutrients for the growth and development of cubs, but also has immune protection functions. Some previous studies have analyzed some nutritional components in captive giant panda breast milk,...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0284
Inventor 王东辉侯蓉刘玉良蔡志刚安俊辉张志和兰景超张皓陈敏李媛何平张瑛
Owner CHENGDU RES BASE OF GIANT PANDA BREEDING
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