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Reagent for inducing peripheral blood mononuclear cells into CIK cells and composition used by reagent

A technology of nuclear cells and composition, applied in the field of biomedicine, can solve the problems of low cell purity and small number of effector cells, and achieve the effect of improving cell viability

Inactive Publication Date: 2019-04-23
北京弘润天源基因生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, IL-2 and anti-CD3 antibodies are the most commonly used factors in CIK expansion, but there are problems such as low cell purity and small number of effector cells.

Method used

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  • Reagent for inducing peripheral blood mononuclear cells into CIK cells and composition used by reagent
  • Reagent for inducing peripheral blood mononuclear cells into CIK cells and composition used by reagent
  • Reagent for inducing peripheral blood mononuclear cells into CIK cells and composition used by reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1. Inducing frozen human peripheral blood mononuclear cells into CIK

[0033] This embodiment provides a set of reagents for inducing CIK from frozen human peripheral blood mononuclear cells. The set of reagents includes reagent 1, reagent 2, reagent 3 and reagent 4, each packaged independently.

[0034] Reagent 1 is KBM581 medium.

[0035] Reagent 2 is a composition for resuscitating frozen human peripheral blood mononuclear cells according to the present invention, the composition is composed of independently packaged human serum albumin and low molecular weight heparin sodium. In reagent 2, human serum albumin and low molecular weight The ratio of heparin sodium is 8mg human serum albumin: 10U low molecular weight heparin sodium. When in use, the composition for resuscitating frozen human peripheral blood mononuclear cells of the present invention is added to KBM581 medium to obtain the resuscitative solution of frozen human peripheral blood mononuclear cells ...

Embodiment 2

[0065] Example 2. Induction of cryopreserved human peripheral blood mononuclear cells from different healthy volunteers into CIK

[0066] The human peripheral blood mononuclear cells of healthy volunteers numbered D1 in Example 1 who were frozen in liquid nitrogen for 3 years were replaced with those of healthy volunteers numbered D2, D3, D4 and D5. For 3-year-old human peripheral blood mononuclear cells, other operations were the same as in Example 1.

[0067]This embodiment provides a set of reagents for inducing CIK from frozen human peripheral blood mononuclear cells. The set of reagents includes reagent 1, reagent 2, reagent 3 and reagent 4, each packaged independently.

[0068] Reagent 1 is KBM581 medium.

[0069] Reagent 2 is a composition for resuscitating frozen human peripheral blood mononuclear cells according to the present invention, the composition is composed of independently packaged human serum albumin and low molecular weight heparin sodium. In reagent 2, hu...

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Abstract

The invention discloses a reagent for inducing peripheral blood mononuclear cells into CIK cells and a composition used by the reagent. The composition for inducing the peripheral blood mononuclear cells into the CIK cells is composed of anti-CD3 antibodies, anti-CD28 antibodies, IL-2, IL-15 and IL-12. The reagent for inducing the peripheral blood mononuclear cells into the CIK cells comprises thecomposition and a freezing peripheral blood mononuclear cell resuscitation solution. By comparing the composition for inducing the human peripheral blood mononuclear cells into the CIK cells with a control composition, it is found that the content of CD3+CD56+ cells in the CIK cells obtained from different healthy volunteers is increased by 72-76%, and the content of CD3+CD8+ cells is increased by 20-21%. By comparing the freezing peripheral blood mononuclear cell resuscitation solution with a conventional resuscitation solution, it is found that the cell viability of the human peripheral blood mononuclear cells can be significantly improved, and the content of CD3+CD56+ cells in the CIK cells obtained from the human peripheral blood mononuclear cells (PBMC) is increased by 47%.

Description

technical field [0001] The invention relates to a reagent for inducing peripheral blood mononuclear cells into CIK and a composition thereof in the field of biomedicine. Background technique [0002] Tumor immunotherapy is a treatment method that uses the body's own immune cells to fight against tumors. It is regarded as the fourth major treatment method in the field of anti-tumor after surgery, radiotherapy, and chemotherapy. In 1991, Schmidt et al. used a variety of cytokines (IFN-γ, IL-2 and anti-CD3 antibody) to co-induce and culture human peripheral blood mononuclear cells in vitro to obtain a group of heterogeneous cells, which have high-efficiency and broad-spectrum killing The function of tumor cells is named as Cytokine-induced killer (CIK). CIK is a group of heterogeneous cells, and its subsets include CD3+CD8+, CD3+CD56+ and other killer cells, among which CD3+CD56+ cells are the main effector cells in CIK, which have strong anti-tumor activity of T lymphocytes a...

Claims

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Application Information

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IPC IPC(8): C12N5/0783C12N5/078
CPCC12N5/0638C12N5/0634C12N2501/2302C12N2501/2312C12N2501/2315C12N2501/24C12N2501/51C12N2501/515C12N2501/91C12N2501/999
Inventor 曹延粉黄庆雷赵伟王培培占天焱沈丽邓钺
Owner 北京弘润天源基因生物技术有限公司
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