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Bifidobacterium longum subsp. longum and application thereof

A technology of bifidobacterium longum and subspecies, applied in the direction of bifidobacteria, applications, bacteria, etc., can solve problems such as neuronal function damage, achieve the effect of alleviating social barriers, improving constipation, and improving cognitive ability

Active Publication Date: 2020-04-28
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Continuous activity and proliferation of glial cells (astrocytes and microglia), and morphological changes, the release of various pro-inflammatory mediators (cytokines and chemokines) to coordinate immune responses and regulate neuronal function and connectivity, while this response can be neuroprotective (i.e., help repair damaged tissue), chronic dysregulation of CNS cytokines can lead to disruption of neuronal function through their roles in cognition and synaptic regulation, These cytokines include IL-6 and TNF-α, etc.

Method used

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  • Bifidobacterium longum subsp. longum and application thereof
  • Bifidobacterium longum subsp. longum and application thereof
  • Bifidobacterium longum subsp. longum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1: Bifidobacterium longum subspecies CCFM1077 has good tolerance to simulated gastrointestinal fluid

[0069] The cryopreserved Bifidobacterium longum subsp. longum CCFM1077 was inoculated in mMRS medium (MRS medium + 0.05% cysteine ​​hydrochloride), cultured anaerobically at 37°C for 48 hours, and then subcultured in mMRS medium After 2 to 3 times, take 1 mL of the culture solution of Bifidobacterium longum subsp. longum CCFM1077, mix it with 9.0 mL of pH 2.5 artificial simulated gastric juice (mMRS medium containing 1% pepsin, pH=2.5), and incubate at 37°C. For anaerobic culture, samples were taken at 0h, 0.5h, 1h and 2h respectively, and the mMRS agar medium was used to pour culture to count plate colonies, determine the number of viable bacteria and calculate their survival rate.

[0070] The survival rate is the ratio of the logarithmic value of the number of viable bacteria at the time of sampling to the logarithmic value of the number of viable bacteria a...

Embodiment 2

[0076] Example 2: Bifidobacterium longum subspecies CCFM1077 has no toxic side effects on wistar rats

[0077] Bifidobacterium longum subsp. longum CCFM1077 was resuspended in 10% skim milk to make a concentration of 3.0×10 8 CFU / mL bacterial suspension. Get 6 male wistar autistic rats with a body weight of about 200g. After one week of acclimatization, the bacterial suspension of this concentration was given by intragastric gavage once a day, observed for one week, and recorded death and body weight.

[0078] The results of these tests are listed in Table 3. These results indicate that feeding concentrations of 3.0×10 8 The Bifidobacterium longum subspecies CCFM1077 of CFU / mL did not cause significant impact on rats, body weight had no significant change, and no death occurred. The appearance of the rats showed no obvious pathological symptoms.

[0079] Table 3 Changes in body weight and death of mice

[0080]

[0081] Note: -: Rats did not die

Embodiment 3

[0082] Example 3: Bifidobacterium longum subspecies CCFM1077 can significantly improve the autonomous exploration ability of autistic rats

[0083] (1) Breeding of young mice with autism

[0084] Healthy male and female wistar rats were bred and adapted to the environment for 1 week. They were caged together at 5:00 p.m. with a male-to-female ratio of 2:1. The vaginal plug was observed at 8:00 the next morning. If no vaginal plug was observed, a vaginal smear was performed. On the first day of pregnancy, the female mice were housed in separate cages after conception, and randomly divided into model group and control group. On the 12.5th day after pregnancy, intraperitoneal injection of sodium valproate (VPA) 600mg / kg (normal saline made into 250mg / mL solution) was given as the model group; Weaning.

[0085]

[0086] (2) Experimental grouping of autistic rats

[0087] Divided into control group, model group and intervention group.

[0088]

[0089] (3) Open field test...

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Abstract

The invention discloses bifidobacterium longum subsp. longum and application thereof. The bifidobacterium longum subsp. longum CCFM1077 can improve the autonomous behavior and exploration behavior ina strange environment caused by autism, relieve social impairment caused by autism, obviously improve the stereotyped behaviors caused by autism, improve the cognitive competence of autism on new objects, improve constipation and effectively adsorb polychlorinated biphenyl.

Description

technical field [0001] The invention belongs to the technical field of functional microorganisms, in particular to Bifidobacterium longum subsp. longum and its application. Background technique [0002] Autism (Autism), also known as autism, has been recognized as a generalized neurodevelopmental disorder that frequently occurs in infants and young children, mainly manifested in social interaction impairment, communication (verbal and non-verbal) function impairment, and repetitive stereotyped behaviors. , often accompanied by a variety of clinical symptoms such as mental retardation, epilepsy or hyperactivity, usually before the age of 3. According to the latest data released by the U.S. Centers for Disease Control and Prevention (CDC), the prevalence of ASD in 2018 was 1:59, an increase of 15% from 1:68 in 2016, and the prevalence rate of men was 4 to 5 times that of women. . [0003] ASD is a highly heterogeneous neurodevelopmental disorder. Genetic factors are the main...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/745A61P25/28A23L33/135C12R1/01
CPCA61K35/745A61P25/28A23L33/135A23V2002/00C12R2001/01C12N1/205A23V2400/533A23V2200/3204A23V2200/30
Inventor 陈卫王刚孔庆敏赵建新张灏
Owner JIANGNAN UNIV
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