Method for efficiently promoting germination of premnapuberula seeds

Abstract

The invention discloses a method for efficiently promoting germination of premnapuberula seeds. The method comprises the following steps: cleaning mature premnapuberula fruits into clean water, removing peel, performing cleaning, and selecting clean seeds for later use; soaking the seeds for later use into a sulfuric acid solution for 3-8 minutes, cleaning the seeds completely with clean water, and sucking off moisture on surfaces of the seeds by using filtering paper for later use; soaking the seeds for later use into a gibberellin solution, taking out the seeds, completely flushing the seedswith clean water, and sucking off moisture on surfaces of the seeds by using filtering paper for later use; flushing the seeds for later use by using 75% alcohol, 0.1% mercury bichloride and sterilewater for multiple times to implement sterilization treatment so as to obtain sterilized seeds for later use; and inoculating the sterilized seeds with an MS (Murashige&Skoog) culture medium, and performing germination culture. By adopting the method, germination difficulties caused by physical dormancy of the premnapuberula seeds can be effectively solved, the germination rate of the premnapuberula seeds can be increased, the germination time can be shortened, then support is provided for on-scale production of the premnapuberula seeds, and in addition, beneficial effects of simplicity in operation and low cost can be achieved.

Description

technical field [0001] The invention relates to a method for germination of the seeds of the body odor, in particular to a method for efficiently promoting the germination of the seeds of the body odor. Background technique [0002] Premnapuberula (Premnapuberula Pamp.), also known as Immortal Tofuchai, is an upright or climbing shrub to small tree belonging to the genus Tofuchai in the Verbenaceae family. Under the wild conditions, the bodily odoriferous wood is heavily felled, and due to the lack of reasonable development, management, and utilization, it is scattered and the number of adult plants continues to decline. Therefore, artificial planting is necessary to realize the industrial development of the body odorous woody resources. [0003] At present, according to the literature, it is difficult for the seeds to reproduce and germinate naturally in the natural state. It has been proved by experiments that the hard seed coat makes the seeds physically dormant, which is...

AI Technical Summary

Problems solved by technology

[0003] At present, according to the literature records, it is difficult for the seeds of Chai chinensis to reproduce and germinate naturally in the natural state. It has been proved by experiments that the hard seed coat makes the seeds physically dormant, which is the main factor limiting its germination.

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