Molecular marker primer for rapidly and efficiently identifying rice salt tolerant gene SKC1 and application

Abstract

The invention discloses a molecular marker primer for rapidly and efficiently identifying a rice salt tolerant gene SKC1 and an application. Specific to the specific difference sites present in the initially reported salt-tolerant (Nona-type) and salt-sensitive (sensitive type) SKC1 gene sequences, a molecular marker primer SKC1-Allele1 / Allele2 / Common for distinguishing the salt-tolerant gene type and sensitive type of the rice salt-resistant gene SKC1 is designed, thereby accurately, quickly and efficiently identifying the SKC1 allele, and providing a powerful tool for the subsequent cultivation of a new strain of salt tolerant rice. Compared with the prior art, the method is more rapid and time-saving, saves the time and effort of an agarose electrophoresis test, and a result can be obtained by only needing 30 seconds of fluorescence scanning after a PCR amplification procedure is finished, the result is clear at a glance, and the accuracy is improved.

Description

technical field [0001] The invention relates to a molecular marker primer for quickly and efficiently identifying rice salt-tolerant gene SKC1 (salt-tolerant type and salt-sensitive type) and its application, belonging to the field of plant biotechnology. Background technique [0002] Rice is one of the most important food crops in the world, and about half of the world's population uses rice as a staple food. During the growth and development of rice, adversities such as low temperature, salinity, drought, pests and diseases will have a serious impact on rice yield and quality. Therefore, research on improving stress resistance is an important way and means to improve rice. There are 1.5 billion mu of barren saline-alkali land in our country, and it is increasing every year. Soil salinization seriously restricts the sustainable development of agriculture in our country. Breeding rice salt-tolerant varieties is of great significance for expanding rice planting area and impr...

AI Technical Summary

Problems solved by technology

Jiang Yiwei et al. (Molecular Plant Breeding, 2018) designed PCR primers through specific SNP sites. Although this method can distinguish the alleles of SKC1, it must be identified and distinguished by agarose electrophoresis test. The test time is prolonged and there are toxic reagents. Reading errors of electrophoretic bands cannot be completely avoided

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