Primer group and kit for detecting novel coronavirus SARS-CoV-2
A coronavirus, sars-cov-2 technology, applied in biochemical equipment and methods, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of complex CrRNA design, expensive RPA reagents, not suitable for widespread promotion, etc. To achieve the effect of simple equipment requirements, low cost and short time consumption
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[0049] 1. Determination of the target gene sequence for detection of novel coronavirus
[0050] Compared the coronavirus strains from Wuhan, bat-SL-CoVZC45, Rs4231 of Chinese chrysanthemum bats, four other SARS virus sequences with the closest homology, human SARS epidemic strains in 2003, civet isolated sequences, common 4 human coronavirus sequences (229E, HKU1, NL63, OC43), etc., the results are as follows figure 1 As shown, the fragment and S gene of the new coronavirus orf1a / b were selected as the target gene for detection.
[0051] The nucleotide sequence of the novel coronavirus orf1a / b fragment is shown in SEQ ID No.15, specifically as follows:
[0052] AAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCAT AAAAACTTTGCAACCAGTATCTGAATTACTTACACCACTGGGC ATTGATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGA TGAGTCTGGTGAGTTTAAATTGGCTTCACATATGTATTGTTCTT TCTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTGAAG AAGAAGAGTTTGAGCCATCAACTCAATATGAGTATGGTACTGA AGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCACTTCT GCTGCTCTTCAAC...
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