A drug and animal model for the intervention of skin photoaging caused by UVA

Abstract

The invention belongs to the technical field of pharmaceutical preparations, and discloses a drug and an animal model for intervening skin photoaging caused by UVA. The drug is indole-3-methanol, and the maximum non-effect dose on HaCaT cells is 10 μmol / L. Take HaCaT cells in the logarithmic growth phase, press 2×10 5 Each well was inoculated in a 6-well culture plate and cultured in a cell culture incubator until the cell confluence was 70-80%; it was divided into groups as follows: the blank control group was covered with opaque black paper, and the UVA model group The irradiation dose is 1J / cm 2 , the I3C treatment group was also treated with 10 μmol / L I3C while irradiating UVA, and two parallel samples were set for each dose; after the irradiation, the changes in the number and shape of cells were observed. The invention can improve the lesions of mouse skin epidermal cells and fibrous tissue caused by excessive UVA irradiation, thus delaying the formation of skin photoaging.

Description

technical field [0001] The invention belongs to the technical field of pharmaceutical preparations, and in particular relates to a drug and an animal model for interfering with UVA-induced skin photoaging. Background technique [0002] Human skin aging is a very complicated process, and genetic factors and environmental factors affect it at the same time. At present, it is found that ultraviolet rays in environmental factors are an important cause of skin aging. Human skin is exposed to ultraviolet radiation from sunlight every day. Studies have shown that long-term ultraviolet radiation will lead to skin photoaging and even skin cancer. With the improvement of human living standards and the growth of the aging population, the problem of skin aging has increasingly attracted people's attention. Due to the increasing destruction of the atmospheric ozone layer and living habits, skin photoaging has become one of the important health problems that threaten modern humans. The ...

AI Technical Summary

Problems solved by technology

However, whether I3C can prevent or delay photoaging through this pathway is still unclear

[0008] Through the above analysis, the problems and defects of the existing technology are: the existing technology is mainly used to solve the chemopreventive effect of various tumors, and has not yet realized its role in preventing, delaying and weakening the photoaging effect caused by ultraviolet rays; whether I3C can Protecting cells, reducing damage, or delaying photoaging through anti-oxidative stress and anti-inflammatory responses is not yet clear

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