Method for analyzing Chinese medicinal dimethyl sulfoxide extract by using capillary electrophoresis technology

Abstract

The invention discloses a method for analyzing Chinese medicinal dimethyl sulfoxide extract by using a capillary electrophoresis technology. The method is characterized by comprising the following steps of: preparing sample solution, namely weighing Chinese medicinal materials or Chinese medicinal preparations according to the specifications, performing pretreatment, substituting the original extracting agent for dimethyl sulfoxide according to the extracting agent quantity specified under the same item, performing extraction by using a common extraction method of Chinese medicaments at the temperature of between 18.45 and 189 DEG C, and filtering the extract to obtain the sample solution; and selecting the capillary electrophoresis type according to the characteristics of the Chinese medicaments by adopting the common capillary electrophoresis technology, and putting the sample solution in a sample injector to start analysis. Through the dimethyl sulfoxide, all components of the Chinese medicinal materials or the Chinese medicinal preparations are extracted quickly at one time by adopting an ultrasonic method at normal temperature higher than 18.45 DEG C; and the dimethyl sulfoxide is a high polar solvent and is very suitable for electrophoresis separation, the Chinese medicinal dimethyl sulfoxide extract can be directly subjected to electrophoresis separation analysis without adding operating buffer solution, the result is a complete undistorted capillary electrophoresis spectrogram containing water-soluble components, oil-soluble components, volatile components, micro components and trace components of the Chinese medicinal materials or the Chinese medicinal preparations, and the capillary electrophoresis spectrogram is a real Chinese medicinal fingerprint diagram.

Description

technical field [0001] The invention relates to a method, in particular to a method for analyzing traditional Chinese medicine dimethyl sulfoxide extract by capillary electrophoresis technology. Background technique [0002] Capillary electrophoresis technology, also known as high-efficiency capillary electrophoresis, uses elastic quartz capillary or flat chip as the separation channel, uses high-voltage direct current electric field as the driving force, and realizes separation according to the differences in mobility and distribution behavior among the components in the sample. New liquid phase separation technology. It includes electrophoresis, chromatography and its cross content, and has the advantages of simple instrument and easy operation; fast analysis speed and high separation efficiency; multiple operation modes, easy development and analysis; low experimental cost, less solvent consumption, and less environmental pollution. [0003] At present, when capillary el...

AI Technical Summary

Problems solved by technology

Due to dilution by buffer solution, trace components and trace components may not be detected by electrophoresis separation, and in the process of water extraction, components such as heat-sensitive, easily hydrolyzed, and easily oxidized may be destroyed, so the obtained electrophoretic spectrum clearly distorted and incomplete

The extract of Chinese herbal medicines or oil-soluble components of traditional Chinese medicine preparations extracted by methanol, ethanol, acetone and other extractants has a different polarity from the operating buffer, so the extract must be evaporated before the extract can be dissolved in the buffer. It is also necessary to add an organic solvent to increase the solubility of the oil-soluble components in the buffer. Although the electrophoretic spectrum of the oil-soluble components of the Chinese medicinal materials or Chinese medicine preparations can be obtained, due to the evaporation of the extractant, the Chinese medicinal materials or Chinese medicine preparations Volatile components are also lost at the same time, so the final result is also distorted and incomplete