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Tartary buckwheat extract identification method

A technology of tartary buckwheat extract and quercetin, which is applied in the field of biomass detection, can solve the problems of difficult identification of tartary buckwheat extract and the like

Active Publication Date: 2020-08-14
JING BRAND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problem that the existing tartary buckwheat extract is difficult to identify, by providing a simple and accurate method for judging the tartary buckwheat extract by high performance liquid chromatography

Method used

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  • Tartary buckwheat extract identification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0070] (1) Instruments and equipment

[0071] High performance liquid chromatography (with UV detector)

[0072] Electronic balance (one hundred thousandth)

[0073] (2) Reagents and materials

[0074] Acetonitrile, phosphoric acid: chromatographically pure

[0075] Methanol, ethanol: analytically pure

[0076] purified water

[0077] (3) Reference chromatographic conditions and system suitability

[0078] a) Chromatographic column: octadecyl bonded silica gel column (250mm*4.6mm, 5μm).

[0079] b) Mobile phase: acetonitrile-0.1% phosphoric acid for gradient elution according to Table 1.

[0080] c) Flow rate: 1.0 mL / min.

[0081] d) Column temperature: 30°C.

[0082] e) Detection wavelength: 360nm.

[0083] f) The number of theoretical plates should not be less than 2000 based on the rutin peak.

[0084] (4) Solution preparation

[0085] Preparation of reference solution

[0086] Accurately weigh 10mg of quercetin-3-rutinoside-7-glucoside into a 100mL volumetric f...

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Abstract

The invention discloses a tartary buckwheat extract identification method. The method can be used to separate five flavonoid components in a tartary buckwheat extract through the same high performanceliquid chromatography condition, and effectively determine the content of characteristic peaks quercetin-3-rutinoside 7-glucoside and rutin in the tartary buckwheat extract, so as to achieve the purpose of tartary buckwheat extract product identification.

Description

technical field [0001] The invention relates to the technical field of biomass detection, in particular to a method for identifying tartary buckwheat extract. Background technique [0002] Tartary buckwheat is tartary buckwheat, which belongs to Polygonaceae, and is a typical embodiment of the homology culture of medicine and food in my country. Tartary buckwheat is known as the "King of the Five Grains", and it is a food that can lower blood pressure, lower blood sugar, and lower blood fat. Tartary buckwheat has unique, comprehensive and rich nutritional components, and has good medicinal properties, and has various nutritional components necessary for the human body. Tartary buckwheat has the effect of laxative and detoxification, and it is also called "cleaning grass" among the people. Tartary buckwheat can also be roasted and made into tea, drinking it every day has an auxiliary therapeutic effect on patients with three highs. Tartary buckwheat is a rare medicinal and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/86
CPCG01N30/06G01N30/8624G01N2030/8648
Inventor 王玉张继斌陈志元徐浪梅菊
Owner JING BRAND
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