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Antibody detection kit, and application thereof in immunoassay

A technology for antibody detection and immune analysis, which is applied in the fields of analytical materials, biological testing, and biological material analysis, and can solve problems such as the inability to directly and clearly examine the expression of PD-1 protein and increase the cost of detection

Pending Publication Date: 2020-08-14
江西赛基生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this kind of kit has a wide variety of antibodies, and it is impossible to observe the expression of PD-1 protein intuitively and clearly, and it increases the detection cost

Method used

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  • Antibody detection kit, and application thereof in immunoassay
  • Antibody detection kit, and application thereof in immunoassay
  • Antibody detection kit, and application thereof in immunoassay

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] The mouse anti-human CD3 monoclonal antibody, mouse anti-human CD4 monoclonal antibody, mouse anti-human CD8 monoclonal antibody, and mouse anti-human PD1 monoclonal antibody of Example 1 were contained in different containers to form detection reagents respectively.

[0063] Specifically, the mouse anti-human CD3 monoclonal antibody, mouse anti-human CD4 monoclonal antibody, mouse anti-human CD8 monoclonal antibody, and mouse anti-human PD1 monoclonal antibody in Example 1 were dissolved in phosphate buffer solution to form the Four liquid reagents are described.

[0064] More specifically, in each of the liquid reagents, the concentration of the mouse anti-human CD3 monoclonal antibody is 2.5 μg / ml, the concentration of the mouse anti-human CD4 monoclonal antibody is 1.5 μg / ml, and the concentration of the mouse anti-human CD8 The monoclonal antibody concentration is 6 μg / ml, and the mouse anti-human PD1 monoclonal antibody concentration is 1 μg / ml.

[0065] The diff...

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PUM

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Abstract

The invention provides an antibody detection kit. The antibody detection kit comprises an antibody of a programmed death receptor 1 and antibodies of at least three cluster differentiation antigens. The antibody of the programmed death receptor 1 and the antibody of each cluster differentiation antigen carry different fluoresceins, the at least three cluster differentiation antigens comprise a mature T cell surface marker, an auxiliary T cell surface marker and a cytotoxic T cell surface marker, and can be used for rapidly investigating the expression condition of PD-1 protein, and the staining index of fluorescein carried by the antibody of the programmed death receptor 1 is higher than that of fluorescein carried by the antibody of each cluster differentiation antigen, so that the expression condition of the PD-1 protein can be accurately investigated. The invention also provides application of the antibody detection kit in immunoassay.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to an antibody detection kit and its application in immune analysis. Background technique [0002] In recent years, tumor immunotherapy has achieved remarkable results. Many clinical studies have confirmed that tumor immunotherapy can improve the prognosis of cancer patients and significantly prolong the survival period of patients. At the same time, the exploration of biomarkers for tumor immunotherapy has also become a hot spot in the research of tumor immunotherapy. [0003] Programmed death-1 (PD-1) is a co-stimulatory molecule that is inducibly expressed on the surface of activated T cells, B cells and NK cells, and PD-1 binds to its ligand PD-L1 After that, it can inhibit the transduction of TCR activation signal, thereby inhibiting the activation, survival and effector function of T lymphocytes, inducing the apoptosis of tumor-specific T lymphocytes, promoting t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/58G01N33/577
CPCG01N33/68G01N33/582G01N33/577G01N2333/70521
Inventor 熊霞熊顺强
Owner 江西赛基生物技术有限公司
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