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Nano material for nucleic acid vaccine enhancer

A technology of nanomaterials and nucleic acid vaccines, applied in the field of nanomaterials, can solve the problems of no nucleic acid vaccine enhancer (efficacy) agent, etc., and achieve the effect of promoting phagocytosis and increasing the probability of contact

Pending Publication Date: 2020-08-25
吴延恒
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There is no report of nucleic acid vaccine enhancer (efficacy) agent

Method used

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  • Nano material for nucleic acid vaccine enhancer
  • Nano material for nucleic acid vaccine enhancer
  • Nano material for nucleic acid vaccine enhancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] This example provides a nanomaterial for a nucleic acid vaccine enhancer, which is prepared by the following method:

[0028] (1) Preparation of A solution: 0.42mL of 2.0M CaCl 2 Dissolve in 2mLddH with 0.84mL pH=10 Trisbuffer with a concentration of 10mM 2 O middle;

[0029] (2) Prepare B solution: mix 0.43mL of HEPES buffer with 0.3mL of ddH 2 O mixed; wherein the HEPES buffer contains a concentration of 280mM NaCl, 15mM Na 2 HPO 4 and 50mM HEPES.

[0030] (3) Add solution B dropwise to solution A, and stir at 500 rpm for 30 minutes to obtain CP nanoparticle precipitation;

[0031] (4) 0.1 mg / mL sodium pamidronate (Pamidronate) is added dropwise to the AB mixed solution of step (3), and the human gamma globulin (Humanγ-globulin) whose concentration is 0.04ug / mL is added dropwise, Stir for 30 minutes to obtain the nanomaterial of the present invention.

Embodiment 2

[0033] The only difference between this example and Example 1 is that the concentration of sodium pamidronate in this example is 1 mg / mL.

Embodiment 3

[0035] The only difference between this example and Example 1 is that the concentration of sodium pamidronate in this example is 3 mg / mL.

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Abstract

The invention provides a nano material for a nucleic acid vaccine enhancer. The nano material is characterized in that the nano material is prepared by the following method of (1) preparing a solutionA: dissolving CaCl2 and Tris buffer in water; (2) preparing a solution B: mixing an HEPES buffer solution with water; (3) dropwise adding the solution B into the solution A, and performing stirring to obtain CP nanoparticle precipitates; and (4) dropwise adding pamidronate sodium into the AB mixed solution obtained in the step (3), and dropwise adding human blood gamma globulin to obtain the nanomaterial disclosed by the invention. According to the nano material, the transfection efficiency of a nucleic acid vaccine on target cells is remarkably improved, antigen expression is increased, antigen presenting cell maturation is promoted, so that an effective immune reaction is established, and the enhancement or synergism function of the nucleic acid vaccine is achieved.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials, and in particular relates to a nanomaterial used as a nucleic acid vaccine enhancer. Background technique [0002] Nucleic acid vaccine is to introduce a foreign gene (DNA or RNA) encoding a certain antigenic protein into animal or human cells, and synthesize the antigenic protein through the expression system of the host cell, and induce the host to generate an immune response to the antigenic protein, so as to prevent and the purpose of treating diseases. Compared with traditional vaccines, nucleic acid vaccines have many advantages. At present, nucleic acid vaccines have entered the clinical stage and even been put on the market. However, how to enable nucleic acid vaccines to reach target cells and effectively express and present antigenic proteins after entering the body has always been a bottleneck that hinders the rapid development and accelerated application of nucleic acid vaccine...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K39/00A61P37/02
CPCA61K39/39A61K39/00A61P37/02A61K2039/55511A61K2039/55516A61K2039/572A61K2039/55555A61K2300/00
Inventor 吴延恒
Owner 吴延恒
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