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Detection method for tetrahydrofolic acid production

A detection method, the technology of tetrahydrofolic acid, is applied in the direction of color/spectral characteristic measurement, analysis through chemical reaction of materials, and material analysis through observation of the influence on chemical indicators, which can solve the problems that are difficult to meet the detection requirements, four Errors in the test results of hydrofolic acid and other problems, to avoid excessive measurement time and improve accuracy

Pending Publication Date: 2020-10-02
无锡紫杉药业股份有限公司
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Problems solved by technology

[0003] In the prior art, there are many detection methods of tetrahydrofolate, but there are some defects among these detection methods, so that there are some large errors in the detection results of tetrahydrofolate, and it is difficult to meet people's detection requirements

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  • Detection method for tetrahydrofolic acid production

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Embodiment Construction

[0034] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention.

[0035] Examples of the described embodiments are shown in the drawings, wherein like or similar reference numerals designate like or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the figures are exemplary and are only used for explaining the patent, and should not be construed as limiting the patent.

[0036] In the description of this patent, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection", "connection", and "setting" should be understood in a broad sense, for example, it can be fixed connection, setting , can also be detach...

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Abstract

The invention discloses a detection method for tetrahydrofolic acid production. The method comprises the following steps: S1, preparing to-be-detected agents and related detection materials: 1-3 partsof to-be-detected agents, an ELISA plate provided with a blank hole, a standard hole and a sample hole to be detected, a plalte sealing film, an FA enzyme marker, a concentrated washing solution, a color developing solution A, a color developing solution B and a stop solution; S2, diluting the to-be-tested agents into a concentrated sample diluent; S3, adding a sample: adding the concentrated sample diluent in the S2 into the blank hole, the standard hole and the to-be-detected sample hole of the ELISA plate; and S4, plate sealing and storage: sealing the ELISA plate with the plate sealing film, and storing at a constant temperature. According to the invention, through multiple tests, the average absorbance value of the sample is compared with a standard value to obtain a more accurate concentration range, and multiple temperature returning treatments in the operation process ensure the activity of enzyme and simulate the normal temperature of a human body, so that the detection result is closer to the reality.

Description

technical field [0001] The invention specifically relates to the technical field of folic acid detection, in particular to a detection method for tetrahydrofolate production. Background technique [0002] Tetrahydrofolate is a reduced form of folic acid, also known as coenzyme F, which is the parent compound of folic acid in the form of coenzyme. When folic acid deficiency or certain drugs inhibit folate reductase, folic acid cannot be converted into tetrahydrofolate, which can affect the development and maturation of blood cells, resulting in megaloblastic anemia. The biosynthetic reaction is blocked; the most typical manifestation of higher animals is that the development and maturation of red blood cells are affected, and megaloblastic anemia is prone to occur; during pregnancy, due to the increase in demand, it can lead to deficiency, and severe deficits can cause fetal development Neural tube defects. Therefore, the production of tetrahydrofolate becomes very importan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/78
CPCG01N21/31G01N21/78
Inventor 葛月兰文佺佺
Owner 无锡紫杉药业股份有限公司
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