Mutation breeding method for Ta-tsai tetraploid

A technology of mutation breeding and tetraploid, which is applied in botany equipment and methods, seed and rhizome treatment, plant genetic improvement, etc., can solve the problems of labor-intensive, low mutagenesis efficiency, time-consuming, etc., and achieve low mutagenesis efficiency , Large number of mutagenesis, easy to keep the seed pure

Pending Publication Date: 2020-10-09
YANGZHOU UNIV
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Problems solved by technology

This method is labor-intensive, time-consuming, and mutagenic efficiency is low

Method used

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  • Mutation breeding method for Ta-tsai tetraploid
  • Mutation breeding method for Ta-tsai tetraploid
  • Mutation breeding method for Ta-tsai tetraploid

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Experimental program
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Embodiment

[0029] 1. Seed mutagenesis treatment:

[0030] The black cabbage seeds are soaked to accelerate germination, and the germination temperature is 20°C. When the seeds germinate and expose the radicle, add 0.03% colchicine to treat for 8 hours, wash off the colchicine, then sow and raise seedlings, and continue to observe the emergence situation.

[0031] 2. Select mutant plants:

[0032] 2.1. After colchicine treatment, the mutated plants were injured, their growth was slow, and their true leaves were shrunken and deformed. The mutated plants were selected and planted, and the management was strengthened. At the same time, untreated diploid black cabbage was planted as a control. When flowering, select plants with late flowering and large petals for pollen identification, and observe the pollen with a microscope. Compared with the diploid pollen, the plants with huge pollen are self-seed, and the first generation of mutagenesis and the first generation of mutagenesis are obtain...

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Abstract

The invention discloses a mutation breeding method for a Ta-tsai tetraploid and belongs to the field of plant breeding. The method comprises the following steps: soaking Ta-tsai seeds and acceleratinggermination, adding 0.03% colchicine when the seeds germinate and the radicle is exposed, after treatment, washing away the colchicine, sowing and raising seedlings to obtain a first generation of mutagenesis; sowing the first generation of mutagenesis for raising seedlings, planting plants for growth, and reserving seeds by selfing to obtain a second generation of mutagenesis; and sowing the second generation of mutagenesis for raising seedlings, planting plants for growth, reserving seeds by selfing to obtain the Ta-tsai tetraploid. The mutation breeding method of the present invention is dedicated to the mutation breeding of tetraploid Ta-tsai. The Ta-tsai tetraploid has high yield, high quality, and strong stress resistance, and the Ta-tsai tetraploid and diploid are not compatible with each other. The Ta-tsai is induced into the tetraploid to ensure that the Ta-tsai tetraploid is not natural crossed with a diploid, good seed breeding and reserved seed purification preservation are easier, the yield and quality are improved, and an excellent new variety is provided for farmers.

Description

technical field [0001] The invention relates to a method for mutation breeding of black cabbage polyploid, in particular to a method for mutation breeding of black cabbage tetraploid, which belongs to the field of plant breeding. Background technique [0002] Artificial mutation breeding has become one of the important means of modern breeding science and has played a huge role in agricultural production. Therefore, in recent decades, people have conducted in-depth research on polyploidy, which not only provides a lot of evidence for evolution, but also has extensive application value in production practice. [0003] Black cabbage, also known as Baoying black cabbage, black cabbage, walnut black cabbage, tataki, tadisong, wutaicai, etc., is mainly distributed in the Yangtze River Basin. , biennial herb, 30-40 cm high, green, all glabrous or occasionally very sparse bristles on the underside of basal leaves; short stems, upper branches. Basal leaves few, dense, short and wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06A01C1/00A01G22/15
CPCA01H1/06A01C1/00A01G22/15
Inventor 成玉富张成成范孟媛施智婕黄钰杨旭
Owner YANGZHOU UNIV
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