Mutation breeding method for Ta-tsai tetraploid
A technology of mutation breeding and tetraploid, which is applied in botany equipment and methods, seed and rhizome treatment, plant genetic improvement, etc., can solve the problems of labor-intensive, low mutagenesis efficiency, time-consuming, etc., and achieve low mutagenesis efficiency , Large number of mutagenesis, easy to keep the seed pure
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[0029] 1. Seed mutagenesis treatment:
[0030] The black cabbage seeds are soaked to accelerate germination, and the germination temperature is 20°C. When the seeds germinate and expose the radicle, add 0.03% colchicine to treat for 8 hours, wash off the colchicine, then sow and raise seedlings, and continue to observe the emergence situation.
[0031] 2. Select mutant plants:
[0032] 2.1. After colchicine treatment, the mutated plants were injured, their growth was slow, and their true leaves were shrunken and deformed. The mutated plants were selected and planted, and the management was strengthened. At the same time, untreated diploid black cabbage was planted as a control. When flowering, select plants with late flowering and large petals for pollen identification, and observe the pollen with a microscope. Compared with the diploid pollen, the plants with huge pollen are self-seed, and the first generation of mutagenesis and the first generation of mutagenesis are obtain...
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