Method for detecting antifungal drugs in serum by ultra-high performance liquid chromatography-tandem mass spectrometry technology

Abstract

The invention discloses a method for detecting antifungal drugs in serum by an ultra-high performance liquid chromatography-tandem mass spectrometry technology. The antifungal drug is prepared from fluconazole (FCZ), voriconazole (VCZ), voriconazole N-oxide (OVCZ), posaconazole (PCZ), itraconazole (ICZ), hydroxyl itraconazole (HICZ) and caspofungin (CPF). The method includes: detecting the contentof the antifungal drug in the pretreated serum by adopting ultra-high performance liquid chromatography tandem mass spectrometry, performing quantifying by utilizing a mass spectrum isotope internalstandard method, establishing a calibration curve by taking the concentration ratio of a standard substance to an internal standard substance as an X axis and the peak area ratio of the standard substance to the internal standard substance as a Y axis, and calculating the concentration of a target drug in the serum; the method is high in sensitivity, high in specificity, accurate and simple in pretreatment process, separation and detection of seven common antifungal drugs are completed at the same time within 5.0 min, the accuracy and precision basically meet the requirements, and a simple andrapid detection method is provided for clinical antifungal drug concentration monitoring.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and more specifically relates to a method for detecting antifungal drugs in serum by ultra-high performance liquid chromatography tandem mass spectrometry. Background technique [0002] Fungal infection is a problem that cannot be ignored clinically. In nosocomial infections, fungal infection accounts for about 7.5%, or even higher. Especially for patients with critical illness, organ transplantation, immunodeficiency, and immunosuppressive therapy. Fungal infections cause very high infection and mortality rates in these patients. There are relatively few antifungal drugs approved for clinical use, and some antifungal drugs have developed drug resistance, making the treatment of antifungal infections more difficult. Therefore, more rational use of existing drugs is needed. Fluconazole (FCZ), voriconazole (VCZ), posaconazole (PCZ), itraconazole (ICZ), and caspofungin (CPF) fungal...

AI Technical Summary

Problems solved by technology

[0003] At present, the methods for detecting the concentration of antifungal drugs in the body mainly include microbial methods, high performance liquid chromatography, and ultra-high performance liquid chromatography-tandem mass spectrometry. Most of the literature reports are for one-time detection of 1-4 drugs, and there are also patent reports. For example, a Chinese patent application (publication number: CN 109030672 A) discloses a "kit for simultaneously measuring four antifungal drugs in dried blood spots and its application", which detects four kinds of antifungal drugs and takes a single sample collection time Long, linear range is not suitable; Chinese patent application (publication number: CN 110470775 A) discloses: "A kit for monitoring drug concentration of fluconazole in blood and its detection method", only for one kind of drug, need to prepare special equipment , poor applicability, large sample size, complex sample pretreatment and matrix effect interference

There are also literature reports on LC-MS/MS dete...

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